Selection of Mycosphaerella fijiensis -resistant cell lines from micro-cross sections of banana and plantain

1997 ◽  
Vol 16 (5) ◽  
pp. 339-343 ◽  
Author(s):  
B. N. Okole
Keyword(s):  
Cell Lines ◽  
Cross Sections ◽  
Resistant Cell ◽  
Mycosphaerella Fijiensis ◽  
Selection Of

Selection of Cell Lines of Catharanthus roseus with Increased Tryptophan Decarboxylase Activity

1983 ◽  
Vol 38 (11-12) ◽  
pp. 916-922 ◽  
Author(s):  
Florenz Sasse ◽  
Marita Buchholz ◽  
Jochen Berlin
Keyword(s):  
Cell Lines ◽  
Indole Alkaloid ◽  
Resistant Cell ◽  
Regulatory Control ◽  
Tryptophan Decarboxylase ◽  
Decarboxylase Activity ◽  
Wild Type ◽  
Feedback Sensitivity ◽  
Tryptophan Analogues ◽  
Selection Of

Cell lines of Catharcmthus roseus were selected for resistance to 4-methyltryptophan (4-MT) in order to get strains with increased activities of tryptophan decarboxylase (TDC). This enzyme may exert a decisive regulatory control in indole alkaloid biosynthesis. 4-Methyltryptophan is a substrate for TDC and is detoxified by this enzyme. All 4-MT resistant cell lines showed increased TDC activity (3-to 10-fold) and higher accumulation of tryptamine (3- to 5-fold). Two of these cell lines with increased TDC activity accumulated indole alkaloids in the growth medium, whereas in wild type cultures and other resistant cell lines this was not the case. All the cell lines obtained were not only resistant to 4-MT (20- to 70-times more than wild type cells) but were also cross-resistant to other tryptophan analogues which are not metabolized by TDC. Therefore the resistance was not only due to a better detoxification of the analogue. The selected cell lines also contained 2 to 6 times higher levels of ʟ-tryptophan but the feedback sensitivity of the anthranilate synthetase of the resistant cells was not alteref

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