Selection of hydroxyproline-resistant cell lines fromSolanum Tuberosum L. Callus. II. Plant regeneration and frost tolerance of regenerated plants

1998 ◽  
Vol 18 (4) ◽  
pp. 361-366 ◽  
Author(s):  
M. A. Anjum
Keyword(s):  
Plant Regeneration ◽  
Cell Lines ◽  
Frost Tolerance ◽  
Resistant Cell ◽  
Regenerated Plants ◽  
Selection Of

Selection of Cell Lines of Catharanthus roseus with Increased Tryptophan Decarboxylase Activity

1983 ◽  
Vol 38 (11-12) ◽  
pp. 916-922 ◽  
Author(s):  
Florenz Sasse ◽  
Marita Buchholz ◽  
Jochen Berlin
Keyword(s):  
Cell Lines ◽  
Indole Alkaloid ◽  
Resistant Cell ◽  
Regulatory Control ◽  
Tryptophan Decarboxylase ◽  
Decarboxylase Activity ◽  
Wild Type ◽  
Feedback Sensitivity ◽  
Tryptophan Analogues ◽  
Selection Of

Cell lines of Catharcmthus roseus were selected for resistance to 4-methyltryptophan (4-MT) in order to get strains with increased activities of tryptophan decarboxylase (TDC). This enzyme may exert a decisive regulatory control in indole alkaloid biosynthesis. 4-Methyltryptophan is a substrate for TDC and is detoxified by this enzyme. All 4-MT resistant cell lines showed increased TDC activity (3-to 10-fold) and higher accumulation of tryptamine (3- to 5-fold). Two of these cell lines with increased TDC activity accumulated indole alkaloids in the growth medium, whereas in wild type cultures and other resistant cell lines this was not the case. All the cell lines obtained were not only resistant to 4-MT (20- to 70-times more than wild type cells) but were also cross-resistant to other tryptophan analogues which are not metabolized by TDC. Therefore the resistance was not only due to a better detoxification of the analogue. The selected cell lines also contained 2 to 6 times higher levels of ʟ-tryptophan but the feedback sensitivity of the anthranilate synthetase of the resistant cells was not alteref

scholarly journals A comparative study on Acorus calamus (Acoraceae) micropropagation and selection of suitable population for cultivation in Iran

2021 ◽  
Vol 65 (1) ◽  
pp. 29-34
Author(s):  
Abbas Gholipour ◽  
Seyed Kamal Kazemitabar ◽  
Hamed Ramzanpour
Keyword(s):  
Plant Regeneration ◽  
Average Length ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Acorus Calamus ◽  
Health Food ◽  
Regenerated Plants ◽  
Sweet Flag ◽  
Medicinal Properties ◽  
Selection Of

In addition to various medicinal properties, Acorus calamus (sweet flag) is used in health, food, and perfume industries. Since this species is a rare plant in Iran, its propagation and cultivation are of the great importance. The aim of this study was to investigate the effects of different plant growth regulators on micropropaga-tion of this plant and to select the appropriate population. The root, the rhizome and the leaf explants of three populations (Arzefon, Pelesk, and Alandan) were cultured on MS medium supplemented with different concentrations of α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) for callus induction and plant regeneration. The results showed that only rhizome explant resulted in direct plant regeneration. Among different treatments, the 1 mg/l treatment of BAP and NAA - with the highest mean number of regenerated plants (3.75 ± 0.85), the highest percentage of grown explants (91.6%) and maximum average length of regenerated plants (12.06 ± 0.32 cm) - was the best treatment for regeneration of sweet flag. The highest mean number of root (6.6 ± 0.1) was observed in Alandan population in 1 mg/l treatment of indole-3-butyric acid (IBA). According to the present study, Alandan population is suitable for cultivation purposes in Iran.

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