Isolation of the glucose oxidase gene from Talaromyces flavus and characterisation of its role in the biocontrol of Verticillium dahliae

1997 ◽  
Vol 32 (5) ◽  
pp. 367-375 ◽  
Author(s):  
F. R. Murray ◽  
D. J. Llewellyn ◽  
W. J. Peacock ◽  
E. S. Dennis
Keyword(s):  
Glucose Oxidase ◽  
Verticillium Dahliae ◽  
Oxidase Gene ◽  
Talaromyces Flavus ◽  
Glucose Oxidase Gene

Genome sequencing of Aureobasidium pullulans P25 and overexpression of a glucose oxidase gene for hyper-production of Ca2+-gluconic acid

2018 ◽  
Vol 112 (5) ◽  
pp. 669-678 ◽  
Author(s):  
Shou-Feng Zhao ◽  
Hong Jiang ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Genome Sequencing ◽  
Gluconic Acid ◽  
Aureobasidium Pullulans ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene

Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae

1990 ◽  
Vol 18 (6) ◽  
pp. 531-536 ◽  
Author(s):  
H. Whittington ◽  
S. Kerry-Williams ◽  
K. Bidgood ◽  
N. Dodsworth ◽  
J. Peberdy ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene

scholarly journals Expression of a Fungal Glucose Oxidase Gene in Three Potato Cultivars with Different Susceptibility to Late Blight (Phytophthora infestans Mont. deBary)

2003 ◽  
Vol 128 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Kimberly J. Felcher ◽  
D.S. Douches ◽  
W.W. Kirk ◽  
R. Hammerschmidt ◽  
W. Li
Keyword(s):  
Enzyme Activity ◽  
Disease Resistance ◽  
Late Blight ◽  
Glucose Oxidase ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Copy Number ◽  
Oxidase Gene ◽  
Transgenic Lines ◽  
Glucose Oxidase Gene

Research was done to determine if enhanced resistance to potato (Solanum tuberosum L.) late blight could be obtained by combining host plant resistance and engineered resistance. Late blight susceptible cultivars, Atlantic, and Spunta and the partially resistant cultivar Libertas were transformed with a fungal glucose oxidase gene, resulting in lines which ranged in transgene copy number from 1 to 8. Glucose oxidase enzyme activity ranged from 0.00 to 96.74×10-5 units/mg plant tissue. There was no correlation between copy number and level of transgene mRNA, level of transgene mRNA and enzyme activity, or between level of enzyme activity and disease resistance. Field and growth chamber evaluation of late blight response demonstrated little to no effect of the glucose oxidase transgene in either late blight susceptible or partially late blight resistant cultivars. However, enzyme activity levels were much lower than levels reported in previous research, which may account for the lack of effect of glucose oxidase against Phytophthora infestans. Twenty-one percent of the transgenic lines were phenotypically off-type compared to nontransgenic controls. Most of the off-type transgenic lines (four out of seven) were derived from `Libertas'. Because several off-type lines did not express the glucose oxidase protein, this phenomenon could not be attributed solely to the glucose oxidase transgene. Based on these results, transgenic lines produced for this study do not increase resistance to P. infestans even in combination with moderate host plant resistance. However, production of greater numbers of transgenic lines with the current construct or, production of transgenic lines in which a different constitutive promoter drives the expression of the glucose oxidase gene might result in greater disease resistance. However, the usefulness of any small increase in resistance would need to be evaluated against the time and cost required for development of transgenic potato cultivars and the potential for off-type tubers and plants.

Expression of Penicillium variabile P16 glucose oxidase gene in Pichia pastoris and characterization of the recombinant enzyme

2006 ◽  
Vol 39 (6) ◽  
pp. 1230-1235 ◽  
Author(s):  
Silvia Crognale ◽  
Valentina Pulci ◽  
Viviana Brozzoli ◽  
Maurizio Petruccioli ◽  
Federico Federici
Keyword(s):  
Pichia Pastoris ◽  
Glucose Oxidase ◽  
Recombinant Enzyme ◽  
Oxidase Gene ◽  
Penicillium Variabile ◽  
Glucose Oxidase Gene
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