Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae

1990 ◽  
Vol 18 (6) ◽  
pp. 531-536 ◽  
Author(s):  
H. Whittington ◽  
S. Kerry-Williams ◽  
K. Bidgood ◽  
N. Dodsworth ◽  
J. Peberdy ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene

scholarly journals Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610

2020 ◽  
Vol 6 (1) ◽  
pp. 10-19
Author(s):  
Popi Asri Kurniatin ◽  
Laksmi Ambarsari ◽  
Annisa Dhiya Athiyyah Khanza ◽  
Inda Setyawati ◽  
Djarot Sasongko Hami Seno ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Gc Content ◽  
Sequence Length ◽  
Oxidase Gene ◽  
E Coli ◽  
Gene Recognition ◽  
Enzymatic Fuel Cell ◽  
Ecori Restriction ◽  
Glucose Oxidase Gene

Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not been conducted. The purpose of this research, therefore, is to identify and characterized the gene responsible for encoding glucose oxidase, in the aspect of sequence, length, and restriction patterns. This experiment involved the amplification of genomic DNA using specific primers for gene recognition, which was followed by the restriction technique with EcoRI and PstI endonucleases. Furthermore, the gene is inserted into vector pGEM®T-Easy and transformed into competent E. coli DH5α cells, in an attempt to perform sequencing. The glucose oxidase gene from A. niger IPBCC 08.610 was confirmed to possess a size of 1848 bp, and a GC content of 57.8%, with a possibility of restriction into two fragments of size 908 bp and 980 bp, using the EcoRI restriction.

Secretory and continuous expression of Aspergillus niger glucose oxidase gene in Pichia pastoris

2007 ◽  
Vol 55 (2) ◽  
pp. 273-278 ◽  
Author(s):  
Masaki Yamaguchi ◽  
Yusuke Tahara ◽  
Atsunori Nakano ◽  
Tadayoshi Taniyama
Keyword(s):  
Aspergillus Niger ◽  
Pichia Pastoris ◽  
Glucose Oxidase ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3

FEBS Letters ◽  
1989 ◽  
Vol 255 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Marion Kriechbaum ◽  
Hans J. Heilmann ◽  
Franz J. Wientjes ◽  
Marina Hahn ◽  
Klaus-D. Jany ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene

Genome sequencing of Aureobasidium pullulans P25 and overexpression of a glucose oxidase gene for hyper-production of Ca2+-gluconic acid

2018 ◽  
Vol 112 (5) ◽  
pp. 669-678 ◽  
Author(s):  
Shou-Feng Zhao ◽  
Hong Jiang ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Genome Sequencing ◽  
Gluconic Acid ◽  
Aureobasidium Pullulans ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene
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