Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase

1997 ◽  
Vol 31 (4) ◽  
pp. 348-356 ◽  
Author(s):  
Jacek Topczewski ◽  
Marzena Sienko ◽  
A. Paszewski
Keyword(s):  
Aspergillus Nidulans ◽  
Gene Encoding ◽  
Cysteine Synthase

Cloning and characterization of the gene encoding Aspergillus nidulans DNA topoisomerase II

Gene ◽  
1999 ◽  
Vol 236 (2) ◽  
pp. 293-301 ◽  
Author(s):  
Kil-Hwan Kim ◽  
Tomohiro Akashi ◽  
Ikuyo Mizuguchi ◽  
Akihiko Kikuchi
Keyword(s):  
Aspergillus Nidulans ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Gene Encoding ◽  
Dna Topoisomerase

scholarly journals Fungal Metabolic Model for Tyrosinemia Type 3: Molecular Characterization of a Gene Encoding a 4-Hydroxy-Phenyl Pyruvate Dioxygenase from Aspergillus nidulans

2006 ◽  
Vol 5 (8) ◽  
pp. 1441-1445 ◽  
Author(s):  
Márcia Eliana da Silva Ferreira ◽  
Marcela Savoldi ◽  
Pierina Sueli Bonato ◽  
Maria Helena S. Goldman ◽  
Gustavo H. Goldman
Keyword(s):  
Aspergillus Nidulans ◽  
Molecular Characterization ◽  
Mutant Strain ◽  
Metabolic Model ◽  
Gene Encoding ◽  
Hereditary Tyrosinemia ◽  
Type 3

ABSTRACT Mutations in the human HPD gene (encoding 4-hydroxyphenylpyruvic acid dioxygenase) cause hereditary tyrosinemia type 3 (HT3). We deleted the Aspergillus nidulans homologue (hpdA). We showed that the mutant strain is not able to grow in the presence of phenylalanine and that it accumulates increased concentrations of tyrosine and 4-hydroxyphenylpyruvic acid, mimicking the human HT3 phenotype.

Isolation and characterization of the Aspergillus nidulans eglC gene encoding a putative β-1,3-endoglucanase

2005 ◽  
Vol 42 (7) ◽  
pp. 590-600 ◽  
Author(s):  
Chang-Jun Choi ◽  
Hee-Jeong Ju ◽  
Byung-Hyun Park ◽  
Rui Qin ◽  
Kwang-Yeop Jahng ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Gene Encoding ◽  
Isolation And Characterization

Characterization of the amyR gene encoding a transcriptional activator for the amylase genes in Aspergillus nidulans

2001 ◽  
Vol 39 (1) ◽  
pp. 10-15 ◽  
Author(s):  
Shuji Tani ◽  
Yoko Katsuyama ◽  
Tomoko Hayashi ◽  
Hayato Suzuki ◽  
Masashi Kato ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Transcriptional Activator ◽  
Gene Encoding ◽  
Amylase Genes

scholarly journals Molecular characterization of the Aspergillus nidulans yA locus.

Genetics ◽  
1989 ◽  
Vol 121 (2) ◽  
pp. 249-254 ◽  
Author(s):  
E B O'Hara ◽  
W E Timberlake
Keyword(s):  
Aspergillus Nidulans ◽  
Gene Disruption ◽  
Molecular Organization ◽  
Dna Fragments ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Mutant Strains ◽  
Low Levels ◽  
Chromosome I

Abstract We investigated the molecular organization of the region of Aspergillus nidulans chromosome I containing yA, a gene encoding the developmentally regulated enzyme conidial laccase. DNA fragments were identified that complemented the yA2 mutation and were shown to correspond to yA by genetic mapping and gene disruption experiments. The molecular map of the region was oriented to the genetic map by testing DNA fragments for their ability to complement a mutation in the tightly linked adE gene. The yA gene codes for a 2200 nucleotide mRNA that is present at low levels in vegetative cells and mature conidia, but accumulates to high levels in sporulating cultures. yA mRNA appears shortly after differentiation of sporogenous phialide cells. It accumulates in two developmentally abnormal mutant strains that produce phialides but is absent from two mutant strains that do not produce phialides. Thus, yA transcription is probably restricted to phialides. This result is discussed in relationship to the physiological roles played by phialides in spore differentiation.

scholarly journals Phosphopantetheinyl Transferase CfwA/NpgA Is Required for Aspergillus nidulans Secondary Metabolism and Asexual Development

2007 ◽  
Vol 6 (4) ◽  
pp. 710-720 ◽  
Author(s):  
Olivia Márquez-Fernández ◽  
Ángel Trigos ◽  
Jose Luis Ramos-Balderas ◽  
Gustavo Viniegra-González ◽  
Holger B. Deising ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolism ◽  
Aspergillus Nidulans ◽  
Large Scale ◽  
Fatty Acid Biosynthesis ◽  
Phosphopantetheinyl Transferase ◽  
Gene Encoding ◽  
Peptide Synthetases ◽  
Isolation And Characterization

ABSTRACT Polyketide synthases (PKSs) and/or nonribosomal peptide synthetases (NRPSs) are central components of secondary metabolism in bacteria, plants, and fungi. In filamentous fungi, diverse PKSs and NRPSs participate in the biosynthesis of secondary metabolites such as pigments, antibiotics, siderophores, and mycotoxins. However, many secondary metabolites as well as the enzymes involved in their production are yet to be discovered. Both PKSs and NRPSs require activation by enzyme members of the 4′-phosphopantetheinyl transferase (PPTase) family. Here, we report the isolation and characterization of Aspergillus nidulans strains carrying conditional (cfwA2) and null (ΔcfwA) mutant alleles of the cfwA gene, encoding an essential PPTase. We identify the polyketides shamixanthone, emericellin, and dehydroaustinol as well as the sterols ergosterol, peroxiergosterol, and cerevisterol in extracts from A. nidulans large-scale cultures. The PPTase CfwA/NpgA was required for the production of these polyketide compounds but dispensable for ergosterol and cerevisterol and for fatty acid biosynthesis. The asexual sporulation defects of cfwA, ΔfluG, and ΔtmpA mutants were not rescued by the cfwA-dependent compounds identified here. However, a cfwA2 mutation enhanced the sporulation defects of both ΔtmpA and ΔfluG single mutants, suggesting that unidentified CfwA-dependent PKSs and/or NRPSs are involved in the production of hitherto-unknown compounds required for sporulation. Our results expand the number of known and predicted secondary metabolites requiring CfwA/NpgA for their biosynthesis and, together with the phylogenetic analysis of fungal PPTases, suggest that a single PPTase is responsible for the activation of all PKSs and NRPSs in A. nidulans.

scholarly journals Cloning and Characterization of the cpyA Gene Encoding Intracellular Carboxypeptidase from Aspergillus nidulans

2001 ◽  
Vol 65 (5) ◽  
pp. 1175-1180 ◽  
Author(s):  
Keisuke OHSUMI ◽  
Yutaka MATSUDA ◽  
Harushi NAKAJIMA ◽  
Katsuhiko KITAMOTO
Keyword(s):  
Aspergillus Nidulans ◽  
Gene Encoding
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