Identification at strain level of Rhizoctonia solani AG4 isolates by direct sequence of asymmetric PCR products of the ITS regions

1996 ◽  
Vol 29 (2) ◽  
pp. 174-181 ◽  
Author(s):  
M. Boysen ◽  
Marisé Borja ◽  
Catalina del Moral ◽  
Oscar Salazar ◽  
V. Rubio
Keyword(s):  
Rhizoctonia Solani ◽  
Strain Level ◽  
Direct Sequence ◽  
Asymmetric Pcr ◽  
Its Regions ◽  
Pcr Products

Identification at strain level ofRhizoctonia solani AG4 isolates by direct sequence of asymmetric PCR products of the ITS regions

1996 ◽  
Vol 29 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Marianne Boysen ◽  
Marisé Borja ◽  
Catalina del Moral ◽  
Oscar Salazar ◽  
Victor Rubio
Keyword(s):  
Strain Level ◽  
Direct Sequence ◽  
Asymmetric Pcr ◽  
Its Regions ◽  
Pcr Products

Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani

1997 ◽  
Vol 32 (3) ◽  
pp. 237-243 ◽  
Author(s):  
S. Kuninaga ◽  
Tomohide Natsuaki ◽  
Toru Takeuchi ◽  
Ryozo Yokosawa
Keyword(s):  
Rhizoctonia Solani ◽  
Sequence Variation ◽  
Anastomosis Groups ◽  
Rdna Its ◽  
Its Regions

Electrochemical Detection of Asymmetric PCR Products by Labeling with Osmium Tetroxide

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Maren Mix ◽  
Thomas Reske ◽  
Heiko Duwensee ◽  
Gerd-Uwe Flechsig
Keyword(s):  
Electrochemical Detection ◽  
Osmium Tetroxide ◽  
Asymmetric Pcr ◽  
Pcr Products

A universal method for directional cloning of PCR products based on asymmetric PCR

2009 ◽  
Vol 52 (1) ◽  
pp. 41 ◽  
Author(s):  
Bao-Li Wang ◽  
Yan-Li Jiao ◽  
Xiao-Xia Li ◽  
Fang Zheng ◽  
Hui Liang ◽  
...  
Keyword(s):  
Universal Method ◽  
Asymmetric Pcr ◽  
Directional Cloning ◽  
Pcr Products

scholarly journals Morphological and Genotypic Characterization of Fungi Associated with the Ascochyta Blight Complex in Western Regions of Algeria

2018 ◽  
Vol 14 (9) ◽  
pp. 276
Author(s):  
A. Tadja
Keyword(s):  
Molecular Weight ◽  
Pisum Sativum ◽  
Ascochyta Blight ◽  
Pathogenic Fungi ◽  
Large Set ◽  
Its Regions ◽  
Pcr Products ◽  
Ascochyta Pinodes ◽  
Pathogenicity Tests ◽  
Ascochyta Pisi

The study is conducted in two growing areas of garden pea (Pisum sativum L.) in northwestern Algeria. Damages caused by Ascochyta sp complex are important in particular for the variety of Kelvedon Wonder. Observations carried out on the infected plants for several years, indicate the presence of superimposed necrosis of different sizes on all aerial organs. However, these observations do not differentiate symptoms by species. The results of morphological and molecular characterization with sequencing in internal transcribed spacer (ITS) regions and inoculation tests on 32 isolates in the laboratory of symbiosis and plant pathology from Toulouse (France), show a reconciliation of the sequencing by polymerase chain reaction (PCR) products and size necrosis for all Ascochyta pinodes and pinodella. Alone, Ascochyta pisi is distinguished by a smaller size necrosis. On the molecular level, all isolates whose ITS regions were amplified by PCR, expresses similar size products (550 bp). This molecular weight is found on a large set of pathogenic fungi. The three species of Ascochyta sp complex do not exhibit polymorphism for Pisum sativum species and have an identical molecular weight. The pathogenicity tests performed showed differences in aggressiveness on the host plant. Ascochyta pinodes is the most aggressive than the other two species. As a result, it causes more damage to the crop.

Determination of HCV genotype by direct sequence analysis of quantitative PCR products

2002 ◽  
Vol 69 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Franco Gargiulo ◽  
Maria Antonia De Francesco ◽  
Gabriele Pinsi ◽  
Caterina Pollara ◽  
Luigina Terlenghi ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Quantitative Pcr ◽  
Direct Sequence ◽  
Hcv Genotype ◽  
Pcr Products ◽  
Direct Sequence Analysis

scholarly journals Characterization of AG-13, a Newly Reported Anastomosis Group of Rhizoctonia solani

2002 ◽  
Vol 92 (8) ◽  
pp. 893-899 ◽  
Author(s):  
D. E. Carling ◽  
R. E. Baird ◽  
R. D. Gitaitis ◽  
K. A. Brainard ◽  
S. Kuninaga
Keyword(s):  
Rhizoctonia Solani ◽  
Internal Transcribed Spacer ◽  
Aerial Mycelium ◽  
Its Region ◽  
The United States ◽  
Anastomosis Group ◽  
Its Regions ◽  
Cotton Plants ◽  
Agar Surface

Rhizoctonia solani anastomosis group (AG)-13 was collected from diseased roots of field grown cotton plants in Georgia in the United States. Isolates of AG-13 did not anastomose with tester isolates of AG-1 through AG-12. Mycelium of all isolates of AG-13 were light brown but darkened as cultures aged. All isolates produced aerial mycelium. Concentric rings were visible after 3 to 4 days of growth but disappeared as cultures aged and darkened. Individual sclerotia were up to 1.5 mm in diameter, similar in color to the mycelium, and generally embedded in the agar. Clumps of sclerotia up to 5 mm in diameter were produced on the agar surface. All attempts to induce basidiospore production were unsuccessful. The 5.8S region of the rDNA from isolates of AG-13 was identical in length and sequence to isolates of all other AGs of R. solani. Length and sequence of the internal transcribed spacer (ITS) regions of rDNA from isolates of AG-13 were unique among AGs of R. solani. Similarity between AG-13 and other AGs of R. solani ranged from 68 to 85% for ITS region 1 and 85 to 95% for ITS region 2. Selected isolates of AG-13 caused minor or no damage to barley, cauliflower, cotton, lettuce, potato, and radish in laboratory or greenhouse studies.

scholarly journals Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers

1993 ◽  
Vol 21 (14) ◽  
pp. 3333-3334 ◽  
Author(s):  
Yao-Guang Liu ◽  
Norihiro Mitsukawa ◽  
Robert F. Whittier
Keyword(s):  
Asymmetric Pcr ◽  
Cycle Sequencing ◽  
Pcr Products
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