Identification at strain level ofRhizoctonia solani AG4 isolates by direct sequence of asymmetric PCR products of the ITS regions

1996 ◽  
Vol 29 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Marianne Boysen ◽  
Marisé Borja ◽  
Catalina del Moral ◽  
Oscar Salazar ◽  
Victor Rubio
Keyword(s):  
Strain Level ◽  
Direct Sequence ◽  
Asymmetric Pcr ◽  
Its Regions ◽  
Pcr Products

Identification at strain level of Rhizoctonia solani AG4 isolates by direct sequence of asymmetric PCR products of the ITS regions

1996 ◽  
Vol 29 (2) ◽  
pp. 174-181 ◽  
Author(s):  
M. Boysen ◽  
Marisé Borja ◽  
Catalina del Moral ◽  
Oscar Salazar ◽  
V. Rubio
Keyword(s):  
Rhizoctonia Solani ◽  
Strain Level ◽  
Direct Sequence ◽  
Asymmetric Pcr ◽  
Its Regions ◽  
Pcr Products

Electrochemical Detection of Asymmetric PCR Products by Labeling with Osmium Tetroxide

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Maren Mix ◽  
Thomas Reske ◽  
Heiko Duwensee ◽  
Gerd-Uwe Flechsig
Keyword(s):  
Electrochemical Detection ◽  
Osmium Tetroxide ◽  
Asymmetric Pcr ◽  
Pcr Products

A universal method for directional cloning of PCR products based on asymmetric PCR

2009 ◽  
Vol 52 (1) ◽  
pp. 41 ◽  
Author(s):  
Bao-Li Wang ◽  
Yan-Li Jiao ◽  
Xiao-Xia Li ◽  
Fang Zheng ◽  
Hui Liang ◽  
...  
Keyword(s):  
Universal Method ◽  
Asymmetric Pcr ◽  
Directional Cloning ◽  
Pcr Products

scholarly journals Morphological and Genotypic Characterization of Fungi Associated with the Ascochyta Blight Complex in Western Regions of Algeria

2018 ◽  
Vol 14 (9) ◽  
pp. 276
Author(s):  
A. Tadja
Keyword(s):  
Molecular Weight ◽  
Pisum Sativum ◽  
Ascochyta Blight ◽  
Pathogenic Fungi ◽  
Large Set ◽  
Its Regions ◽  
Pcr Products ◽  
Ascochyta Pinodes ◽  
Pathogenicity Tests ◽  
Ascochyta Pisi

The study is conducted in two growing areas of garden pea (Pisum sativum L.) in northwestern Algeria. Damages caused by Ascochyta sp complex are important in particular for the variety of Kelvedon Wonder. Observations carried out on the infected plants for several years, indicate the presence of superimposed necrosis of different sizes on all aerial organs. However, these observations do not differentiate symptoms by species. The results of morphological and molecular characterization with sequencing in internal transcribed spacer (ITS) regions and inoculation tests on 32 isolates in the laboratory of symbiosis and plant pathology from Toulouse (France), show a reconciliation of the sequencing by polymerase chain reaction (PCR) products and size necrosis for all Ascochyta pinodes and pinodella. Alone, Ascochyta pisi is distinguished by a smaller size necrosis. On the molecular level, all isolates whose ITS regions were amplified by PCR, expresses similar size products (550 bp). This molecular weight is found on a large set of pathogenic fungi. The three species of Ascochyta sp complex do not exhibit polymorphism for Pisum sativum species and have an identical molecular weight. The pathogenicity tests performed showed differences in aggressiveness on the host plant. Ascochyta pinodes is the most aggressive than the other two species. As a result, it causes more damage to the crop.

Determination of HCV genotype by direct sequence analysis of quantitative PCR products

2002 ◽  
Vol 69 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Franco Gargiulo ◽  
Maria Antonia De Francesco ◽  
Gabriele Pinsi ◽  
Caterina Pollara ◽  
Luigina Terlenghi ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Quantitative Pcr ◽  
Direct Sequence ◽  
Hcv Genotype ◽  
Pcr Products ◽  
Direct Sequence Analysis

scholarly journals Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers

1993 ◽  
Vol 21 (14) ◽  
pp. 3333-3334 ◽  
Author(s):  
Yao-Guang Liu ◽  
Norihiro Mitsukawa ◽  
Robert F. Whittier
Keyword(s):  
Asymmetric Pcr ◽  
Cycle Sequencing ◽  
Pcr Products

Abnormal Processing of the Glycoprotein IIb Transcript due to a Nonsense Mutation in Exon 17 Associated with Glanzmann’s Thrombasthenia

1995 ◽  
Vol 73 (05) ◽  
pp. 756-762 ◽  
Author(s):  
Yoshiaki Tomiyama ◽  
Hirokazu Kashiwagi ◽  
Satoru Kosugi ◽  
Masamichi Shiraga ◽  
Yoshio Kanayama ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Molecular Genetic ◽  
Genetic Defect ◽  
Nucleotide Sequence Analysis ◽  
Type I ◽  
Normal Amount ◽  
Immunoblot Assay ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Pcr Products

SummaryWe analyzed the molecular genetic defect responsible for type I Glanzmann’s thrombasthenia in a Japanese patient. In an immunoblot assay using polyclonal anti-GPIIb-IIIa antibodies, some GPIIIa (15% of normal amount) could be detected in the patient’s platelets, whereas GPIIb could not (<2% of normal amount). Nucleotide sequence analysis of platelet GPIIb mRNA-derived polymerase chain reaction (PCR) products revealed that patient’s GPIIb cDNA had a 75-bp deletion in the 3’ boundary of exon 17 resulting in an in-frame deletion of 25 amino acids. DNA analysis and family study revealed that the patient was a compound heterozygote of two GPIIb gene defects. One allele derived from her father was not expressed in platelets, and the other allele derived from her mother had a 9644C → T mutation which was located at the position -3 of the splice donor junction of exon 17 and resulted in a termination codon (TGA). Moreover, quantitative analysis demonstrated that the amount of the abnormal GPIIb transcript in the patient’s platelets was markedly reduced. Thus, the C → T mutation resulting in the abnormal splicing of GPIIb transcript and the reduction in its amount is responsible for Glanzmann’s thrombasthenia.

MOLECULAR STUDY OF HUMAN HEAD LICE, PEDICULUS HUMANUS CAPITIS COMPARISON WITH GOAT SUCKING LICE, LINOGNATHUS STENOPSIS (BURMEISTER) STRAINS

2020 ◽  
pp. 132-139
Keyword(s):  
Genetic Difference ◽  
Human Head ◽  
Molecular Study ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
School Students ◽  
Pediculus Humanus ◽  
Sucking Lice ◽  
Pcr Products ◽  
Amplicon Size

In this study, only (122) out of (915) primary school students were shown to be infected with head lice Pediculus. humanus capitis. The number and percentage of infected males were 46 (11.3%), while the number and percentage of infected females were 76 (14.9%). The results in our study also showed that the number and percentage of goats infected with goat sucking lice, Linognathus stenopsis was 70 (21.7%) of the total 322 animals, with the highest number and percentage among female goats 44 (62.9%) compared to the male goats 26 (37.1%). The study demonstrated that the rate of genetic difference between the studied samples was 89% and the similarity rate was 11%. Detection of OP-K01 gene pieces by PCR products showed that the amplicon size was 520 bp for P. humanus capitis isolated from humans, while the detection of OP-E20 and OP-M05 gene pieces with PCR product showed the lowest amplicon size 230 bp for Linognathus stenosis isolated from goats.

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