Molecular Characterization of Genes Encoding a Novel ABC Transporter in Thermoanaerobacterium thermosulfurigenes EM1

1997 ◽  
Vol 35 (4) ◽  
pp. 237-239 ◽  
Author(s):  
Markus Matuschek ◽  
Kerstin Sahm ◽  
Hubert Bahl
Keyword(s):  
Molecular Characterization ◽  
Abc Transporter ◽  
Genes Encoding

scholarly journals Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

Gene ◽  
2011 ◽  
Vol 489 (2) ◽  
pp. 70-75 ◽  
Author(s):  
Tatiane S. Soares ◽  
Renata M.O. Watanabe ◽  
Francisco J.A. Lemos ◽  
Aparecida S. Tanaka
Keyword(s):  
Aedes Aegypti ◽  
Molecular Characterization ◽  
Digestive Enzymes ◽  
Genes Encoding

scholarly journals Molecular characterization of genes encoding isoamylase-type debranching enzyme in tuberous root of sweet potato, Ipomoea batatas (L.) Lam.

2016 ◽  
Vol 33 (5) ◽  
pp. 351-359
Author(s):  
Moe Nabemoto ◽  
Riho Watanabe ◽  
Mizuho Ohsu ◽  
Kaname Sato ◽  
Motoyasu Otani ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Tuberous Root ◽  
Debranching Enzyme ◽  
Genes Encoding

scholarly journals Identification and Characterization of Genes Encoding a Putative ABC-Type Transporter Essential for Utilization of γ-Hexachlorocyclohexane in Sphingobium japonicum UT26

2007 ◽  
Vol 189 (10) ◽  
pp. 3712-3720 ◽  
Author(s):  
Ryo Endo ◽  
Yoshiyuki Ohtsubo ◽  
Masataka Tsuda ◽  
Yuji Nagata
Keyword(s):  
Abc Transporter ◽  
Sole Source ◽  
Bacterial Cells ◽  
New Genes ◽  
Hydrophobic Compounds ◽  
Dead End ◽  
Genes Encoding ◽  
Degradation Activity ◽  
Mutant Cells

ABSTRACT Sphingobium japonicum UT26 utilizes γ-hexachlorocyclohexane (γ-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the conversion of γ-HCH to β-ketoadipate in UT26. In this study, we analyzed a mutant obtained by transposon mutagenesis and identified and characterized new genes encoding a putative ABC-type transporter essential for the utilization of γ-HCH in strain UT26. This putative ABC transporter consists of four components, permease, ATPase, periplasmic protein, and lipoprotein, encoded by linK, linL, linM, and linN, respectively. Mutation and complementation analyses indicated that all the linKLMN genes are required, probably as a set, for γ-HCH utilization in UT26. Furthermore, the mutant cells deficient in this putative ABC transporter showed (i) higher γ-HCH degradation activity and greater accumulation of the toxic dead-end product 2,5-dichlorophenol (2,5-DCP), (ii) higher sensitivity to 2,5-DCP itself, and (iii) higher permeability of hydrophobic compounds than the wild-type cells. These results strongly suggested that LinKLMN are involved in γ-HCH utilization by controlling membrane hydrophobicity. This study clearly demonstrated that a cellular factor besides catabolic enzymes and transcriptional regulators is essential for utilization of xenobiotic compounds in bacterial cells.

scholarly journals Characterization of the Erwinia chrysanthemi gan Locus, Involved in Galactan Catabolism

2007 ◽  
Vol 189 (19) ◽  
pp. 7053-7061 ◽  
Author(s):  
Aurélie Delangle ◽  
Anne-France Prouvost ◽  
Virginie Cogez ◽  
Jean-Pierre Bohin ◽  
Jean-Marie Lacroix ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Transport System ◽  
Pathogenic Bacteria ◽  
Erwinia Chrysanthemi ◽  
Potato Tubers ◽  
Inner Membrane ◽  
Saintpaulia Ionantha ◽  
Genes Encoding

ABSTRACT β-1,4-Galactan is a major component of the ramified regions of pectin. Analysis of the genome of the plant pathogenic bacteria Erwinia chrysanthemi revealed the presence of a cluster of eight genes encoding proteins potentially involved in galactan utilization. The predicted transport system would comprise a specific porin GanL and an ABC transporter made of four proteins, GanFGK2. Degradation of galactans would be catalyzed by the periplasmic 1,4-β-endogalactanase GanA, which released oligogalactans from trimer to hexamer. After their transport through the inner membrane, oligogalactans would be degraded into galactose by the cytoplasmic 1,4-β-exogalactanase GanB. Mutants affected for the porin or endogalactanase were unable to grow on galactans, but they grew on galactose and on a mixture of galactotriose, galactotetraose, galactopentaose, and galactohexaose. Mutants affected for the periplasmic galactan binding protein, the transporter ATPase, or the exogalactanase were only able to grow on galactose. Thus, the phenotypes of these mutants confirmed the functionality of the gan locus in transport and catabolism of galactans. These mutations did not affect the virulence of E. chrysanthemi on chicory leaves, potato tubers, or Saintpaulia ionantha, suggesting an accessory role of galactan utilization in the bacterial pathogeny.

scholarly journals Molecular characterization of the genes encoding acetohydroxy acid synthase in the cyanobacterium Spirulina platensis

1992 ◽  
Vol 138 (7) ◽  
pp. 1399-1408 ◽  
Author(s):  
A. MILANO ◽  
E. DE ROSSI ◽  
E. ZANARIA ◽  
L. BARBIERATO ◽  
O. CIFERRI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spirulina Platensis ◽  
Acetohydroxy Acid Synthase ◽  
Genes Encoding
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