scholarly journals Molecular characterization of the genes encoding DNA gyrase and topoisomerase IV of Listeria monocytogenes

2002 ◽  
Vol 49 (6) ◽  
pp. 917-924 ◽  
Author(s):  
R. Lampidis
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Dna Gyrase ◽  
Topoisomerase Iv ◽  
Genes Encoding

scholarly journals Characterization of Mutations in DNA Gyrase and Topoisomerase IV Involved in Quinolone Resistance of Mycoplasma gallisepticum Mutants Obtained In Vitro

2002 ◽  
Vol 46 (2) ◽  
pp. 590-593 ◽  
Author(s):  
A. K. Reinhardt ◽  
C. M. Bébéar ◽  
M. Kobisch ◽  
I. Kempf ◽  
A. V. Gautier-Bouchardon
Keyword(s):  
Target Genes ◽  
Dna Gyrase ◽  
Mycoplasma Gallisepticum ◽  
Quinolone Resistance ◽  
Mycoplasma Species ◽  
Topoisomerase Iv ◽  
Genes Encoding ◽  
Resistant Mutants

ABSTRACT Mycoplasma gallisepticum enrofloxacin-resistant mutants were generated by stepwise selection in increasing concentrations of enrofloxacin. Alterations were found in the quinolone resistance-determining regions of the four target genes encoding DNA gyrase and topoisomerase IV from these mutants. This is the first description of such mutations in an animal mycoplasma species.

scholarly journals In Vitro Characterization of the Antibacterial Spectrum of Novel Bacterial Type II Topoisomerase Inhibitors of the Aminobenzimidazole Class

2006 ◽  
Vol 50 (4) ◽  
pp. 1228-1237 ◽  
Author(s):  
Nagraj Mani ◽  
Christian H. Gross ◽  
Jonathan D. Parsons ◽  
Brian Hanzelka ◽  
Ute Müh ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Wide Spectrum ◽  
Dna Gyrase ◽  
Antibacterial Activities ◽  
Mechanisms Of Action ◽  
Fluoroquinolone Resistance ◽  
Topoisomerase Iv ◽  
Low Frequencies

ABSTRACT Antibiotics with novel mechanisms of action are becoming increasingly important in the battle against bacterial resistance to all currently used classes of antibiotics. Bacterial DNA gyrase and topoisomerase IV (topoIV) are the familiar targets of fluoroquinolone and coumarin antibiotics. Here we present the characterization of two members of a new class of synthetic bacterial topoII ATPase inhibitors: VRT-125853 and VRT-752586. These aminobenzimidazole compounds were potent inhibitors of both DNA gyrase and topoIV and had excellent antibacterial activities against a wide spectrum of problematic pathogens responsible for both nosocomial and community-acquired infections, including staphylococci, streptococci, enterococci, and mycobacteria. Consistent with the novelty of their structures and mechanisms of action, antibacterial potency was unaffected by commonly encountered resistance phenotypes, including fluoroquinolone resistance. In time-kill assays, VRT-125853 and VRT-752586 were bactericidal against Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis, and Haemophilus influenzae, causing 3-log reductions in viable cells within 24 h. Finally, similar to the fluoroquinolones, relatively low frequencies of spontaneous resistance to VRT-125853 and VRT-752586 were found, a property consistent with their in vitro dual-targeting activities.

scholarly journals Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

Gene ◽  
2011 ◽  
Vol 489 (2) ◽  
pp. 70-75 ◽  
Author(s):  
Tatiane S. Soares ◽  
Renata M.O. Watanabe ◽  
Francisco J.A. Lemos ◽  
Aparecida S. Tanaka
Keyword(s):  
Aedes Aegypti ◽  
Molecular Characterization ◽  
Digestive Enzymes ◽  
Genes Encoding

scholarly journals Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011–2014

2019 ◽  
Vol 79 ◽  
pp. 116-122 ◽  
Author(s):  
Ivana Zuber ◽  
Brankica Lakicevic ◽  
Ariane Pietzka ◽  
Dubravka Milanov ◽  
Vesna Djordjevic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Small Scale

scholarly journals Molecular characterization of genes encoding isoamylase-type debranching enzyme in tuberous root of sweet potato, Ipomoea batatas (L.) Lam.

2016 ◽  
Vol 33 (5) ◽  
pp. 351-359
Author(s):  
Moe Nabemoto ◽  
Riho Watanabe ◽  
Mizuho Ohsu ◽  
Kaname Sato ◽  
Motoyasu Otani ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Tuberous Root ◽  
Debranching Enzyme ◽  
Genes Encoding

scholarly journals Cloning, Expression, and Enzymatic Characterization of Pseudomonas aeruginosaTopoisomerase IV

1999 ◽  
Vol 43 (3) ◽  
pp. 530-536 ◽  
Author(s):  
Takaaki Akasaka ◽  
Yoshikuni Onodera ◽  
Mayumi Tanaka ◽  
Kenichi Sato
Keyword(s):  
Dna Gyrase ◽  
Fusion System ◽  
Enzymatic Characterization ◽  
Wild Type ◽  
Inhibitory Effects ◽  
Topoisomerase Iv ◽  
Upstream Gene ◽  
E Coli ◽  
New Quinolones

ABSTRACT The topoisomerase IV subunit A gene, parC homolog, has been cloned and sequenced from Pseudomonas aeruginosa PAO1, with cDNA encoding the N-terminal region of Escherichia coli parC used as a probe. The homolog and its upstream gene were presumed to be parC and parE through sequence homology with the parC and parE genes of other organisms. The deduced amino acid sequence of ParC and ParE showed 33 and 32% identity with that of the P. aeruginosa DNA gyrase subunits, GyrA and GyrB, respectively, and 69 and 75% identity with that of E. coli ParC and ParE, respectively. The putative ParC and ParE proteins were overexpressed and separately purified by use of a fusion system with a maltose-binding protein, and their enzymatic properties were examined. The reconstituted enzyme had ATP-dependent decatenation activity, which is the main catalytic activity of bacterial topoisomerase IV, and relaxing activities but had no supercoiling activity. So, the cloned genes were identified asP. aeruginosa topoisomerase IV genes. The inhibitory effects of quinolones on the activities of topoisomerase IV and DNA gyrase were compared. The 50% inhibitory concentrations of quinolones for the decatenation activity of topoisomerase IV were from five to eight times higher than those for the supercoiling activities ofP. aeruginosa DNA gyrase. These results confirmed that topoisomerase IV is less sensitive to fluoroquinolones than is DNA gyrase and may be a secondary target of new quinolones in wild-typeP. aeruginosa.

scholarly journals Topoisomerase Mutations in Fluoroquinolone-Resistant and Methicillin-Susceptible and -Resistant Clinical Isolates of Staphylococcus aureus

1998 ◽  
Vol 42 (1) ◽  
pp. 197-198 ◽  
Author(s):  
Glenn W. Kaatz ◽  
Susan M. Seo
Keyword(s):  
Staphylococcus Aureus ◽  
Restriction Fragment Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Dna Gyrase ◽  
Clinical Isolates ◽  
Polymorphism Analysis ◽  
Topoisomerase Iv ◽  
Genes Encoding ◽  
Resistant Strains ◽  
Restriction Fragment Length

ABSTRACT The incidence of the various mutations in the genes encoding topoisomerase IV and DNA gyrase in fluoroquinolone-resistant clinical isolates of Staphylococcus aureus is not known. Using restriction fragment length polymorphism analysis and DNA sequencing, we found that in fluoroquinolone- and methicillin-resistant strains, mutations in grlA and gyrA are quite likely to be present together. For fluoroquinolone-resistant but methicillin-susceptible strains, mutations in grlA alone are more common.

scholarly journals Molecular characterization of the genes encoding acetohydroxy acid synthase in the cyanobacterium Spirulina platensis

1992 ◽  
Vol 138 (7) ◽  
pp. 1399-1408 ◽  
Author(s):  
A. MILANO ◽  
E. DE ROSSI ◽  
E. ZANARIA ◽  
L. BARBIERATO ◽  
O. CIFERRI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spirulina Platensis ◽  
Acetohydroxy Acid Synthase ◽  
Genes Encoding
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