Purification and properties of a novel raw starch degrading cyclomaltodextrin glucanotransferase from Bacillus firmus

1999 ◽  
Vol 51 (4) ◽  
pp. 504-509 ◽  
Author(s):  
B. N. Gawande ◽  
A. Goel ◽  
A. Y. Patkar ◽  
S. N. Nene
Keyword(s):  
Bacillus Firmus ◽  
Raw Starch ◽  
Purification And Properties ◽  
Cyclomaltodextrin Glucanotransferase

Sequencing, cloning, and heterologous expression of cyclomaltodextrin glucanotransferase of Bacillus firmus strain 37 in Bacillus subtilis WB800

2019 ◽  
Vol 42 (4) ◽  
pp. 621-629 ◽  
Author(s):  
Gabriela Gregolin Gimenez ◽  
Hernán Costa ◽  
Quirino Alves de Lima Neto ◽  
Maria Aparecida Fernandez ◽  
Susana Alicia Ferrarotti ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Heterologous Expression ◽  
Bacillus Firmus ◽  
Cyclomaltodextrin Glucanotransferase

Purification and properties of the raw-starch-digesting glucoamylases from Corticium rolfsii

1998 ◽  
Vol 50 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Y. Nagasaka ◽  
K. Kurosawa ◽  
A. Yokota ◽  
F. Tomita
Keyword(s):  
Raw Starch ◽  
Purification And Properties

scholarly journals Purification and Properties of the Raw Starch Degrading α-amylase of Mutant strain: Bacillus cereus 1306

2004 ◽  
Vol 2 (2) ◽  
pp. 46-54
Author(s):  
Manoj Trivedi ◽  
S. K. Mandal
Keyword(s):  
Bacillus Cereus ◽  
Incubation Temperature ◽  
Gel Filtration ◽  
Tween 80 ◽  
Maximum Activity ◽  
Raw Starch ◽  
Purification And Properties ◽  
Amylase Production ◽  
Ph Range ◽  
Triton X

The raw starch-degrading α-amylase Produced by Bacillus cereus 1306 was purified to homogeneity by acetone precipitation and gel filtration chromatography.The Molecular weight of α-amylase was estimated to be 58KDa. The enzyme displayed maximum activity 85 Units/ml at pH 7.0 and an incubation temperature of 37˚C and Stable in the pH range of 5.0-9.0. Activity was inhibited in the presence of Hg2+,Cu2+,Fe3+ but no inhibition was observed in the presence of Zn2+. Medium containing CaCl2.2H2O enhanced amylase production over that on Ca2+-deficient medium. The detergent Tween-80 and Triton X-100 increased Biomass but Significantly Suppressed amylase production. The enzyme released large amount of glucose and maltose on hydrolysis of starch.

Sign in / Sign up

Export Citation Format

Share Document