A novel cyclomaltodextrin glucanotransferase from Bacillus firmus that degrades raw starch

1995 ◽  
Vol 17 (4) ◽  
pp. 411-416 ◽  
Author(s):  
Anuj Goel ◽  
Sanjay Nene
Keyword(s):  
Bacillus Firmus ◽  
Raw Starch ◽  
Cyclomaltodextrin Glucanotransferase

Sequencing, cloning, and heterologous expression of cyclomaltodextrin glucanotransferase of Bacillus firmus strain 37 in Bacillus subtilis WB800

2019 ◽  
Vol 42 (4) ◽  
pp. 621-629 ◽  
Author(s):  
Gabriela Gregolin Gimenez ◽  
Hernán Costa ◽  
Quirino Alves de Lima Neto ◽  
Maria Aparecida Fernandez ◽  
Susana Alicia Ferrarotti ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Heterologous Expression ◽  
Bacillus Firmus ◽  
Cyclomaltodextrin Glucanotransferase

Optimization of cyclomaltodextrin glucanotransferase production from Bacillus firmus

1998 ◽  
Vol 22 (4) ◽  
pp. 288-291 ◽  
Author(s):  
B.N. Gawande ◽  
R.K. Singh ◽  
A.K. Chauhan ◽  
A. Goel ◽  
A.Y. Patkar
Keyword(s):  
Bacillus Firmus ◽  
Cyclomaltodextrin Glucanotransferase

scholarly journals Introduction of Raw Starch-Binding Domains intoBacillus subtilis α-Amylase by Fusion with the Starch-Binding Domain of Bacillus Cyclomaltodextrin Glucanotransferase

2000 ◽  
Vol 66 (7) ◽  
pp. 3058-3064 ◽  
Author(s):  
Kohji Ohdan ◽  
Takashi Kuriki ◽  
Hiroki Takata ◽  
Hiroki Kaneko ◽  
Shigetaka Okada
Keyword(s):  
Catalytic Domain ◽  
Specific Activity ◽  
Binding Domain ◽  
Raw Starch ◽  
Chimeric Enzymes ◽  
Binding Domains ◽  
Molar Basis ◽  
Effects Of Ph ◽  
Raw Starch Binding ◽  
Cyclomaltodextrin Glucanotransferase

ABSTRACT We constructed two types of chimeric enzymes, Ch1 Amy and Ch2 Amy. Ch1 Amy consisted of a catalytic domain of Bacillus subtilis X-23 α-amylase (Ba-S) and the raw starch-binding domain (domain E) of Bacillus A2-5a cyclomaltodextrin glucanotransferase (A2-5a CGT). Ch2 Amy consisted of Ba-S and D (function unknown) plus E domains of A2-5a CGT. Ch1 Amy acquired raw starch-binding and -digesting abilities which were not present in the catalytic part (Ba-S). Furthermore, the specific activity of Ch1 Amy was almost identical when enzyme activity was evaluated on a molar basis. Although Ch2 Amy exhibited even higher raw starch-binding and -digesting abilities than Ch1 Amy, the specific activity was lower than that of Ba-S. We did not detect any differences in other enzymatic characteristics (amylolytic pattern, transglycosylation ability, effects of pH, and temperature on stability and activity) among Ba-S, Ch1 Amy, and Ch2 Amy.

scholarly journals Application of raw starch degrading enzyme from Laceyella sacchari LP175 for development of bacterial cellulose fermentation using colored rice as substrate

3 Biotech ◽  
2021 ◽  
Vol 11 (3) ◽  
Author(s):  
Sirilak Noree ◽  
Chantanan Tongdang ◽  
Kanaporn Sujarit ◽  
Songphon Chamdit ◽  
Voranuch Thongpool ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Raw Starch ◽  
Degrading Enzyme ◽  
Cellulose Fermentation ◽  
Colored Rice

scholarly journals Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Morakot Krajang ◽  
Kwanruthai Malairuang ◽  
Jatuporn Sukna ◽  
Krongchan Rattanapradit ◽  
Saethawat Chamsart
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Consolidated Bioprocessing ◽  
Scale Up ◽  
Cassava Starch ◽  
Single Step ◽  
Starch Hydrolysis ◽  
Yield Coefficient ◽  
Raw Starch ◽  
Raw Starch Hydrolysis

Abstract Background A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigate the optimum conditions and technical procedures for the production of ethanol from raw cassava starch in a single step. It successfully resulted in high yields and productivities of all the experiments from the laboratory, the pilot, through the industrial scales. Yields of ethanol concentration are comparable with those in the commercial industries that use molasses and hydrolyzed starch as the raw materials. Results Before single-step ethanol production, studies of raw cassava starch hydrolysis by a granular starch hydrolyzing enzyme, StargenTM002, were carefully conducted. It successfully converted 80.19% (w/v) of raw cassava starch to glucose at a concentration of 176.41 g/L with a productivity at 2.45 g/L/h when it was pretreated at 60 °C for 1 h with 0.10% (v/w dry starch basis) of Distillase ASP before hydrolysis. The single-step ethanol production at 34 °C in a 5-L fermenter showed that Saccharomyces cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, pmax at 81.86 g/L (10.37% v/v) with a yield coefficient, Yp/s of 0.43 g/g, a productivity or production rate, rp at 1.14 g/L/h and an efficiency, Ef of 75.29%. Scale-up experiments of the single-step ethanol production using this method, from the 5-L fermenter to the 200-L fermenter and further to the 3000-L industrial fermenter were successfully achieved with essentially good results. The values of pmax,Yp/s, rp, and Ef of the 200-L scale were at 80.85 g/L (10.25% v/v), 0.42 g/g, 1.12 g/L/h and 74.40%, respectively, and those of the 3000-L scale were at 70.74 g/L (8.97% v/v), 0.38 g/g, 0.98 g/L/h and 67.56%, respectively. Because of using raw starch, major by-products, i.e., glycerol, lactic acid, and acetic acid of all three scales were very low, in ranges of 0.940–1.140, 0.046–0.052, 0.000–0.059 (% w/v), respectively, where are less than those values in the industries. Conclusion The single-step ethanol production using the combination of raw cassava starch hydrolysis and fermentation of three fermentation scales in this study is practicable and feasible for the scale-up of industrial production of ethanol from raw starch.

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