Purification and properties of the raw-starch-digesting glucoamylases from Corticium rolfsii

1998 ◽  
Vol 50 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Y. Nagasaka ◽  
K. Kurosawa ◽  
A. Yokota ◽  
F. Tomita
2004 ◽  
Vol 2 (2) ◽  
pp. 46-54
Author(s):  
Manoj Trivedi ◽  
S. K. Mandal

The raw starch-degrading α-amylase Produced by Bacillus cereus 1306 was purified to homogeneity by acetone precipitation and gel filtration chromatography.The Molecular weight of α-amylase was estimated to be 58KDa. The enzyme displayed maximum activity 85 Units/ml at pH 7.0 and an incubation temperature of 37˚C and Stable in the pH range of 5.0-9.0. Activity was inhibited in the presence of Hg2+,Cu2+,Fe3+ but no inhibition was observed in the presence of Zn2+. Medium containing CaCl2.2H2O enhanced amylase production over that on Ca2+-deficient medium. The detergent Tween-80 and Triton X-100 increased Biomass but Significantly Suppressed amylase production. The enzyme released large amount of glucose and maltose on hydrolysis of starch.


1991 ◽  
Vol 81 (3) ◽  
pp. 327-334 ◽  
Author(s):  
Cesar V. Mujer ◽  
Dale W. Kretchman ◽  
A. Raymond Miller

1995 ◽  
Vol 94 (4) ◽  
pp. 629-634 ◽  
Author(s):  
Mohamed Benichou ◽  
Gracia Martinez-Reina ◽  
Felix Romojaro ◽  
Jean-Claude Pech ◽  
Alain Latche

Uirusu ◽  
1967 ◽  
Vol 17 (3) ◽  
pp. 118-124 ◽  
Author(s):  
Tohru NISHIHARA ◽  
Itaru WATANABE

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