Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble

2001 ◽  
Vol 56 (1-2) ◽  
pp. 201-204 ◽  
Author(s):  
K. Bartholomew ◽  
G. Dos Santos ◽  
T. Dumonceaux ◽  
T. Charles ◽  
F. Archibald
Keyword(s):  
Genetic Transformation ◽  
Trametes Versicolor ◽  
Selectable Marker ◽  
Dominant Selectable Marker

A new dominant selectable marker for genetic transformation; Hsp70-opd

1995 ◽  
Vol 25 (10) ◽  
pp. 1061-1065 ◽  
Author(s):  
Mark Q. Benedict ◽  
Cristina E. Salazar ◽  
Frank H. Collins
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Dominant Selectable Marker

Hygromycin B resistance as dominant selectable marker in yeast

1984 ◽  
Vol 8 (5) ◽  
pp. 353-358 ◽  
Author(s):  
Kevin R. Kaster ◽  
Stanley G. Burgett ◽  
Thomas D. Ingolia
Keyword(s):  
Selectable Marker ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Dominant Selectable Marker

Insertional mutagenesis in Coprinus cinereus : use of a dominant selectable marker to generate tagged, sporulation-defective mutants

1999 ◽  
Vol 36 (6) ◽  
pp. 371-382 ◽  
Author(s):  
W. Jason Cummings ◽  
Martina Celerin ◽  
Jennifer Crodian ◽  
Linda K. Brunick ◽  
Miriam E. Zolan
Keyword(s):  
Insertional Mutagenesis ◽  
Selectable Marker ◽  
Coprinus Cinereus ◽  
Dominant Selectable Marker

The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation

1994 ◽  
Vol 14 (6) ◽  
pp. 4011-4019
Author(s):  
J A Nelson ◽  
P B Savereide ◽  
P A Lefebvre
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Selectable Marker ◽  
Marker Gene ◽  
Small Subunit ◽  
Direct Selection ◽  
Nuclear Transformation ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Dominant Selectable Marker ◽  
Cry1 Gene

We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant to the eukaryotic translational inhibitors cryptopleurine and emetine in which the resistance was due to a missense mutation (CRY1-1) in the CRY1 gene; resistance was dominant in heterozygous stable diploids. Cotransformation experiments using the CRY1-1 gene and the gene for nitrate reductase (NIT1) produced a low level of resistance to cryptopleurine and emetine. Resistance levels were increased when the CRY1-1 gene was placed under the control of a constitutive promoter from the ribulose bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) gene. We also found that the 5' untranslated region of the CRY1 gene was required for expression of the CRY1-1 transgene. Direct selection of emetine-resistant transformants was possible when transformed cells were first induced to differentiate into gametes by nitrogen starvation and then allowed to dedifferentiate back to vegetative cells before emetine selection was applied. With this transformation protocol, the RBCS2/CRY1-1 dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii.

scholarly journals Bleomycin resistance: a new dominant selectable marker for plant cell transformation

1986 ◽  
Vol 7 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Jacques Hille ◽  
Frank Verheggen ◽  
Peter Roelvink ◽  
Henk Franssen ◽  
Ab van Kammen ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Transformation ◽  
Selectable Marker ◽  
Dominant Selectable Marker ◽  
Plant Cell Transformation

Extinction and activation of the thyroglobulin promoter in hybrids of differentiated and transformed thyroid cells

1990 ◽  
Vol 10 (3) ◽  
pp. 1033-1040
Author(s):  
I M Bonapace ◽  
M Sanchez ◽  
S Obici ◽  
A Gallo ◽  
S Garofalo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Selectable Marker ◽  
Hybrid Cell ◽  
Transformed Cells ◽  
Thyroid Cells ◽  
Dominant Selectable Marker ◽  
Thyroglobulin Gene ◽  
Rat Thyroid ◽  
Hybrid Cell Lines

Thyroglobulin gene expression was repressed in a rat thyroid cell line transformed with Kirsten murine sarcoma virus. Expression of a dominant selectable marker driven by the thyroglobulin promoter was also inhibited. Somatic cell hybridization of transformed and differentiated thyroid cells resulted in extinction of thyroglobulin gene expression. When transformed cells carrying a dominant selectable marker driven by the thyroglobulin promoter were fused to differentiated cells and expression of this marker was selected, we obtained stable hybrid cell lines expressing both the endogenous and the exogenous thyroglobulin promoters. Although the expression of v-ras remained unchanged compared with expression in the parental transformed cells, transformation was suppressed in the hybrid cell lines. The other thyroid differentiation markers, iodide uptake and thyroid-stimulating hormone-dependent growth, were inhibited in all the hybrids tested. We show that activity of the thyroglobulin promoter correlates with the presence of a thyroid nuclear factor that binds the promoter at position -60 from the transcription start site. Loss of this factor accompanies the extinction of thyroglobulin gene expression in hybrids selected for expression of a non-thyroid-specific promoter.

scholarly journals The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae

Metabolites ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 49 ◽  
Author(s):  
Ana Molina-Márquez ◽  
Marta Vila ◽  
Javier Vigara ◽  
Ana Borrero ◽  
Rosa León
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Genetic Manipulation ◽  
Plant Cells ◽  
Phytoene Desaturase ◽  
Carotenoid Content ◽  
Great Promise ◽  
Encoding Gene ◽  
Average Transformation ◽  
Selection Of

Genetic manipulation shows great promise to further boost the productivity of microalgae-based compounds. However, selection of microalgal transformants depends mainly on the use of antibiotics, which have raised concerns about their potential impacts on human health and the environment. We propose the use of a synthetic phytoene desaturase-encoding gene (CRTIop) as a selectable marker and the bleaching herbicide norflurazon as a selective agent for the genetic transformation of microalgae. Bacterial phytoene desaturase (CRTI), which, unlike plant and algae phytoene desaturase (PDS), is not sensitive to norflurazon, catalyzes the conversion of the colorless carotenoid phytoene into lycopene. Although the expression of CRTI has been described to increase the carotenoid content in plant cells, its use as a selectable marker has never been testedin algae or in plants. In this study, a version of the CRTI gene adapted to the codon usage of Chlamydomonas has been synthesized, and its suitability to be used as selectable marker has been shown. The microalgae were transformed by the glass bead agitation method and selected in the presence of norflurazon. Average transformation efficiencies of 550 colonies µg−1 DNA were obtained. All the transformants tested had incorporated the CRTIop gene in their genomes and were able to synthesize colored carotenoids.

Sign in / Sign up

Export Citation Format

Share Document