Electrorotation of Isolated Generative and Vegetative Cells, and of Intact Pollen Grains of Lilium longiflorum

1998 ◽  
Vol 161 (1) ◽  
pp. 21-32 ◽  
Author(s):  
V.L. Sukhorukov ◽  
R. Benkert ◽  
G. Obermeyer ◽  
F.-W. Bentrup ◽  
U. Zimmermann
Keyword(s):  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Vegetative Cells

scholarly journals Impact of Manganese on Pollen Germination and Tube Growth in Lily

2021 ◽  
Vol 74 ◽  
Author(s):  
Thomas Sawidis ◽  
Gülriz Baycu ◽  
Elżbieta Weryszko-Chmielewska ◽  
Aneta Sulborska
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Surrounding Medium ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Low Concentrations ◽  
Main Effect

Abstract In vitro culture of Lilium longiflorum pollen grains was carried out to determine the role of manganese in pollen germination and pollen tube growth. Pollen germination was adversely affected by the presence of manganese (>10 −8 M), whereas low concentrations (10 −12 –10 −10 M) stimulated the process. Manganese caused morphological anomalies during tube growth, characterized by irregular pollen tube thickening and swollen tips. The main effect was the anomalous cell wall formation at the tip, in which the presence of several organelles reduced the number of secretory vesicles. A loose network of fibrillar material and spherical aggregates, mostly in the tip region, was detected, and this material was progressively loosened into the surrounding medium. As a response to potential toxicity, the excess manganese was isolated in vacuoles, which formed an internal barrier against penetration of manganese to the tip area. Elevated manganese concentrations might affect plant reproduction, resulting in anomalies in gamete development. Consequently, the loss in genetic diversity and decreased fruit set ultimately lower yield.

scholarly journals 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nino F. Läubli ◽  
Jan T. Burri ◽  
Julian Marquard ◽  
Hannes Vogler ◽  
Gabriella Mosca ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Cells ◽  
Three Dimensional ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Force Sensor ◽  
Cellular Growth ◽  
Apparent Stiffness ◽  
Micromechanical Characterization

AbstractQuantitative micromechanical characterization of single cells and multicellular tissues or organisms is of fundamental importance to the study of cellular growth, morphogenesis, and cell-cell interactions. However, due to limited manipulation capabilities at the microscale, systems used for mechanical characterizations struggle to provide complete three-dimensional coverage of individual specimens. Here, we combine an acoustically driven manipulation device with a micro-force sensor to freely rotate biological samples and quantify mechanical properties at multiple regions of interest within a specimen. The versatility of this tool is demonstrated through the analysis of single Lilium longiflorum pollen grains, in combination with numerical simulations, and individual Caenorhabditis elegans nematodes. It reveals local variations in apparent stiffness for single specimens, providing previously inaccessible information and datasets on mechanical properties that serve as the basis for biophysical modelling and allow deeper insights into the biomechanics of these living systems.

Nondiffusional Release of Allergens from Pollen Grains of Artemisia vulgaris and Lilium longiflorum Depends Mainly on the Type of the Allergen

2005 ◽  
Vol 137 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Claudia Hoidn ◽  
Erika Puchner ◽  
Heidi Pertl ◽  
Elisabeth Holztrattner ◽  
Gerhard Obermeyer
Keyword(s):  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Artemisia Vulgaris

Purification and characterization of histone H1 from meiotic cells of a monocotyledonous plant, Lilium longiflorum

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 736-741 ◽  
Author(s):  
C. Daniel Riggs
Keyword(s):  
Lilium Longiflorum ◽  
Cell Types ◽  
Histone H1 ◽  
Cellular Protein ◽  
Vegetative Cells ◽  
Unbound Fraction ◽  
Total Cellular Protein ◽  
The Difference ◽  
Different Cell Types ◽  
Sulfuric Acid Solutions

To identify molecules involved in regulating meiotic chromatin structure, nuclear proteins from meiocytes of Lilium longiflorum were chromatographed on hydroxylapatite and the bound and unbound proteins were examined. An abundant nuclear protein was purified from the unbound fraction and by the following criteria was identified as a histone H1 molecule. The protein is soluble in acidic (perchloric and sulfuric acid) solutions, and its amino acid composition and the sequence of its amino terminus are similar to that of known histone H1s. An antiserum was produced against the protein to facilitate subsequent studies. Immunoblotting experiments demonstrated that histone H1 immunostaining declines in the developmental interval spanning the diplotene to tetrads stages. Concommitant with this decline is the appearance of several lower molecular mass, cross-reacting proteins. Neither the identity nor roles of these proteins is known. Immunoblotting experiments also demonstrate that, while the level of the protein is relatively constant in nuclei prepared from meiotic and vegetative cells, histone H1 is apparently enriched in total cellular extracts of meiotic cells compared with vegetative cells. This difference was found to be at least 16-fold. I conclude that in meiotic cells, histone H1 accounts for more of the total cellular protein than it does in vegetative cells. The difference in its relative abundance as a percent of the total cellular protein is probably in part due to differences in the ratio of nuclear to cytoplasmic volume in the different cell types, or the purging of sporophytic proteins from the cytoplasm of the meiocytes, or both.Key words: meiosis, histone H1, immunoblotting, meiotic purging.

Introduction and differential use of various promoters in pollen grains of Nicotiana glutinosa and Lilium longiflorum

1992 ◽  
Vol 11 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Leonne M. van der Leede-Plegt ◽  
Bernadette C. E. van de Ven ◽  
Raoul J. Bino ◽  
Theo P. M. van der Salm ◽  
Arjen J. van Tunen
Keyword(s):  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Nicotiana Glutinosa

Isolation of sporopollenin from four myxobacteria

1977 ◽  
Vol 23 (8) ◽  
pp. 1080-1083 ◽  
Author(s):  
William R. Strohl ◽  
John M. Larkin ◽  
Barry H. Good ◽  
Russell L. Chapman
Keyword(s):  
Pollen Grains ◽  
The Other ◽  
Vegetative Cells

Sporopollenin, a resistance material previously found only in algae, fungi, pollen grains, and in some plant spores, has been found in four species of Myxococcus. Sporopollenin was isolated from vegetative cells and myxospores of the myxobacteria tested but it was not detected in any of the other bacteria tested.

Identification of lily pollen 14-3-3 isoforms and their subcellular and time-dependent expression profile

2011 ◽  
Vol 392 (3) ◽  
Author(s):  
Heidi Pertl ◽  
Simon Rittmann ◽  
Waltraud X. Schulze ◽  
Gerhard Obermeyer
Keyword(s):  
Expression Profile ◽  
Pollen Germination ◽  
Temporal Dynamics ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Mass Spectrometry Analysis ◽  
Primary Metabolism ◽  
Two Dimensional Gel Electrophoresis ◽  
Spectrometry Analysis ◽  
Lily Pollen

Abstract 14-3-3 proteins are major regulators in plant development and physiology including primary metabolism and signal transduction pathways, typically via a phosphorylation-dependent interaction with a target protein. Four full-length 14-3-3 isoforms were identified in pollen grains of Lilium longiflorum by screening of a cDNA library and RACE (rapid amplification of cDNA ends)-PCR. Mass spectrometry analysis of partially purified 14-3-3s confirmed the presence of the four isoforms but also indicated the presence of additional, less abundant 14-3-3 isoforms in lily pollen. Separation of partially purified 14-3-3 proteins by two-dimensional gel electrophoresis resulted in nine spots that mainly contained the four major 14-3-3 isoforms. In a first step to examine putative physiological roles of specific 14-3-3 isoforms, their subcellular expression profile during pollen germination and tube growth was monitored using a characterized set of antibodies against 14-3-3 proteins with distinct crossreactivity. The abundance profile of 14-3-3 proteins associated with the cytosol, endomembranes (tonoplast, endoplasmic reticulum, Golgi, mitochondria) and plasma membrane showed high spatial-temporal dynamics. This indicates different targets of 14-3-3 proteins at different organelles and time points during pollen germination and growth.

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