Introduction and differential use of various promoters in pollen grains of Nicotiana glutinosa and Lilium longiflorum

1992 ◽  
Vol 11 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Leonne M. van der Leede-Plegt ◽  
Bernadette C. E. van de Ven ◽  
Raoul J. Bino ◽  
Theo P. M. van der Salm ◽  
Arjen J. van Tunen
Keyword(s):  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Nicotiana Glutinosa

scholarly journals Impact of Manganese on Pollen Germination and Tube Growth in Lily

2021 ◽  
Vol 74 ◽  
Author(s):  
Thomas Sawidis ◽  
Gülriz Baycu ◽  
Elżbieta Weryszko-Chmielewska ◽  
Aneta Sulborska
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Surrounding Medium ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Low Concentrations ◽  
Main Effect

Abstract In vitro culture of Lilium longiflorum pollen grains was carried out to determine the role of manganese in pollen germination and pollen tube growth. Pollen germination was adversely affected by the presence of manganese (>10 −8 M), whereas low concentrations (10 −12 –10 −10 M) stimulated the process. Manganese caused morphological anomalies during tube growth, characterized by irregular pollen tube thickening and swollen tips. The main effect was the anomalous cell wall formation at the tip, in which the presence of several organelles reduced the number of secretory vesicles. A loose network of fibrillar material and spherical aggregates, mostly in the tip region, was detected, and this material was progressively loosened into the surrounding medium. As a response to potential toxicity, the excess manganese was isolated in vacuoles, which formed an internal barrier against penetration of manganese to the tip area. Elevated manganese concentrations might affect plant reproduction, resulting in anomalies in gamete development. Consequently, the loss in genetic diversity and decreased fruit set ultimately lower yield.

Electrorotation of Isolated Generative and Vegetative Cells, and of Intact Pollen Grains of Lilium longiflorum

1998 ◽  
Vol 161 (1) ◽  
pp. 21-32 ◽  
Author(s):  
V.L. Sukhorukov ◽  
R. Benkert ◽  
G. Obermeyer ◽  
F.-W. Bentrup ◽  
U. Zimmermann
Keyword(s):  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Vegetative Cells

scholarly journals 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nino F. Läubli ◽  
Jan T. Burri ◽  
Julian Marquard ◽  
Hannes Vogler ◽  
Gabriella Mosca ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Cells ◽  
Three Dimensional ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Force Sensor ◽  
Cellular Growth ◽  
Apparent Stiffness ◽  
Micromechanical Characterization

AbstractQuantitative micromechanical characterization of single cells and multicellular tissues or organisms is of fundamental importance to the study of cellular growth, morphogenesis, and cell-cell interactions. However, due to limited manipulation capabilities at the microscale, systems used for mechanical characterizations struggle to provide complete three-dimensional coverage of individual specimens. Here, we combine an acoustically driven manipulation device with a micro-force sensor to freely rotate biological samples and quantify mechanical properties at multiple regions of interest within a specimen. The versatility of this tool is demonstrated through the analysis of single Lilium longiflorum pollen grains, in combination with numerical simulations, and individual Caenorhabditis elegans nematodes. It reveals local variations in apparent stiffness for single specimens, providing previously inaccessible information and datasets on mechanical properties that serve as the basis for biophysical modelling and allow deeper insights into the biomechanics of these living systems.

Nondiffusional Release of Allergens from Pollen Grains of Artemisia vulgaris and Lilium longiflorum Depends Mainly on the Type of the Allergen

2005 ◽  
Vol 137 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Claudia Hoidn ◽  
Erika Puchner ◽  
Heidi Pertl ◽  
Elisabeth Holztrattner ◽  
Gerhard Obermeyer
Keyword(s):  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Artemisia Vulgaris

Identification of lily pollen 14-3-3 isoforms and their subcellular and time-dependent expression profile

2011 ◽  
Vol 392 (3) ◽  
Author(s):  
Heidi Pertl ◽  
Simon Rittmann ◽  
Waltraud X. Schulze ◽  
Gerhard Obermeyer
Keyword(s):  
Expression Profile ◽  
Pollen Germination ◽  
Temporal Dynamics ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Mass Spectrometry Analysis ◽  
Primary Metabolism ◽  
Two Dimensional Gel Electrophoresis ◽  
Spectrometry Analysis ◽  
Lily Pollen

Abstract 14-3-3 proteins are major regulators in plant development and physiology including primary metabolism and signal transduction pathways, typically via a phosphorylation-dependent interaction with a target protein. Four full-length 14-3-3 isoforms were identified in pollen grains of Lilium longiflorum by screening of a cDNA library and RACE (rapid amplification of cDNA ends)-PCR. Mass spectrometry analysis of partially purified 14-3-3s confirmed the presence of the four isoforms but also indicated the presence of additional, less abundant 14-3-3 isoforms in lily pollen. Separation of partially purified 14-3-3 proteins by two-dimensional gel electrophoresis resulted in nine spots that mainly contained the four major 14-3-3 isoforms. In a first step to examine putative physiological roles of specific 14-3-3 isoforms, their subcellular expression profile during pollen germination and tube growth was monitored using a characterized set of antibodies against 14-3-3 proteins with distinct crossreactivity. The abundance profile of 14-3-3 proteins associated with the cytosol, endomembranes (tonoplast, endoplasmic reticulum, Golgi, mitochondria) and plasma membrane showed high spatial-temporal dynamics. This indicates different targets of 14-3-3 proteins at different organelles and time points during pollen germination and growth.

Resolution of Channels in the Exine by Translocation of Colloidal Iron

1971 ◽  
Vol 29 ◽  
pp. 352-353
Author(s):  
John R. Rowley
Keyword(s):  
Phosphate Buffer ◽  
Pollen Development ◽  
Osmium Tetroxide ◽  
Pollen Grains ◽  
Deionized Water ◽  
Colloidal Iron ◽  
Fixed Material ◽  
Epilobium Angustifolium ◽  
Untreated Material ◽  
Microspore Mitosis

The morphology of the exine of many pollen grains, at the time of flowering, is such that one can suppose that transport of substances through the exine occurred during pollen development. Holes or channels, microscopic to submicroscopic, are described for a large number of grains. An inner part of the exine of Epilobium angustifolium L. and E. montanum L., which may be referred to as the endexine, has irregularly shaped channels early in pollen development although by microspore mitosis there is no indication of such channeling in chemically fixed material. The nucleus in microspores used in the experiment reported here was in prophase of microspore mitosis and the endexine, while lamellated in untreated grains, did not contain irregularly shaped channels. Untreated material from the same part of the inflorescence as iron treated stamens was examined following fixation with 0.1M glutaraldehyde in cacodylate-HCl buffer at pH 6.9 (315 milliosmoles) for 24 hrs, 4% formaldehyde in phosphate buffer at pH 7.2 (1,300 milliosmoles) for 12 hrs, 1% glutaraldehyde mixed with 0.1% osmium tetroxide for 20 min, osmium tetroxide in deionized water for 2 hrs and 1% glutaraldehyde mixed with 4% formaldehyde in 0.1M cacodylate-HCl buffer at pH 6.9 for two hrs.

Freeze-substitution of rye grass and ragweed pollen grains

1990 ◽  
Vol 48 (3) ◽  
pp. 686-687
Author(s):  
Liza B. Martinez ◽  
Susan M. Wick
Keyword(s):  
Cell Function ◽  
Pollen Grains ◽  
Freeze Substitution ◽  
Cell Types ◽  
Rapid Freezing ◽  
Rye Grass ◽  
Acrylic Resins ◽  
Allergenic Proteins ◽  
Cold Embedding ◽  
Structural Preservation

Rapid freezing and freeze-substitution have been employed as alternatives to chemical fixation because of the improved structural preservation obtained in various cell types. This has been attributed to biomolecular immobilization derived from the extremely rapid arrest of cell function. These methods allow the elimination of conventionally used fixatives, which may have denaturing or “masking” effects on proteins. Thus, this makes them ideal techniques for immunocytochemistry, in which preservation of both ultrastructure and antigenicity are important. These procedures are also compatible with cold embedding acrylic resins which are known to increase sensitivity in immunolabelling.This study reveals how rapid freezing and freeze-substitution may prove to be useful in the study of the mobile allergenic proteins of rye grass and ragweed. Most studies have relied on the use of osmium tetroxide to achieve the necessary ultrastructural detail in pollen whereas those that omitted it have had to contend with poor overall preservation.

Origin of Nectar and Pollen Grains in Honey Produced in Basrah Governorate

2014 ◽  
Vol 27 (1) ◽  
pp. 245-253
Author(s):  
Muhannad R. J. Allamy ◽  
TTaha Y. Al-Edany
Keyword(s):  
Pollen Grains
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