Synthesis of glycosylated porphyrins as neutral ionophores for a berberine-sensitive electrode

2001 ◽  
Vol 369 (5) ◽  
pp. 422-427 ◽  
Author(s):  
X.-B. Zhang ◽  
C.-C. Guo ◽  
S.-H. Chen ◽  
G.-L. Shen ◽  
R.-Q. Yu
Keyword(s):  
Sensitive Electrode ◽  
Glycosylated Porphyrins

Determination of Factor XIII Activity and of Factor XIII Inhibitors Using an Ammonium-Sensitive Electrode

1983 ◽  
Vol 50 (02) ◽  
pp. 563-566 ◽  
Author(s):  
P Hellstern ◽  
K Schilz ◽  
G von Blohn ◽  
E Wenzel
Keyword(s):  
Factor Xiii ◽  
Normal Subjects ◽  
The Other ◽  
Activity Measurement ◽  
Factor Xiii Deficiency ◽  
Assay Procedure ◽  
Stabilization Factor ◽  
Release Method ◽  
Sensitive Electrode

SummaryAn assay for rapid factor XIII activity measurement has been developed based on the determination of the ammonium released during fibrin stabilization. Factor XIII was activated by thrombin and calcium. Ammonium was measured by an ammonium-sensitive electrode. It was demonstrated that the assay procedure yields accurate and precise results and that factor XIII-catalyzed fibrin stabilization can be measured kinetically. The amount of ammonium released during the first 90 min of fibrin stabilization was found to be 7.8 ± 0.5 moles per mole fibrinogen, which is in agreement with the findings of other authors. In 15 normal subjects and in 15 patients suffering from diseases with suspected factor XIII deficiency there was a satisfactory correlation between the results obtained by the “ammonium-release-method”, Bohn’s method, and the immunological assay (r1 = 0.65; r2= 0.70; p<0.01). In 3 of 5 patients with paraproteinemias the values of factor XIII activity determined by the ammonium-release method were markedly lower than those estimated by the other methods. It could be shown that inhibitor mechanisms were responsible for these discrepancies.

scholarly journals Encapsulation of glycosylated porphyrins in silica nanoparticles to enhance the efficacy of cancer photodynamic therapy

2021 ◽  
Author(s):  
Wioleta Borzęcka ◽  
Patrícia M. R. Pereira ◽  
Rosa Fernandes ◽  
Tito Trindade ◽  
Tomás Torres ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Silica Nanoparticles ◽  
Glycosylated Porphyrins

Encapsulation of glycosylated porphyrins in silica nanoparticles enhanced the efficacy of cancer photodynamic therapy compared with the free porphyrins.

scholarly journals Convenient Approach to Access Octa-Glycosylated Porphyrins via “Click Chemistry”

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Misako Okada ◽  
Yuko Kishibe ◽  
Kanako Ide ◽  
Toshiyuki Takahashi ◽  
Teruaki Hasegawa
Keyword(s):  
Click Chemistry ◽  
Water Solubility ◽  
One Pot ◽  
Lectin Affinity ◽  
Convenient Approach ◽  
Porphyrin Core ◽  
Glycosylated Porphyrins

Easy, quantitative, and one-pot introduction of eight β-lactoside-modules onto a porphyrin-core was achieved through Cu+-catalyzed chemoselective coupling (click chemistry) between a porphyrin carrying eight alkyne-terminals and β-lactosyl azides. The obtained porphyrin-based glycocluster shows not only good water-solubility but also strong/specific lectin-affinity.

Mutant analysis suggests that cyclic GMP mediates the cyclic AMP-induced Ca2+ uptake in Dictyostelium

1991 ◽  
Vol 99 (1) ◽  
pp. 187-191
Author(s):  
S. Menz ◽  
J. Bumann ◽  
E. Jaworski ◽  
D. Malchow
Keyword(s):  
Cyclic Amp ◽  
Mutant Strain ◽  
Cyclic Gmp ◽  
Parental Strain ◽  
Dependent Manner ◽  
Heavy Chains ◽  
Signal Relay ◽  
Sensitive Electrode ◽  
Dose Dependent ◽  
Cyclic Amp Synthesis

Previous work has shown that streamer F (stmF) mutants of Dictyostelium discoideum exhibit prolonged chemotactic elongation in aggregation fields. The mutants carry an altered structural gene for cyclic GMP phosphodiesterase resulting in low activities of this enzyme. Chemotactic stimulation by cyclic AMP causes a rapid transient increase in the cyclic GMP concentration followed by association of myosin heavy chains with the cytoskeleton. Both events persist several times longer in stmF mutants than in the parental strain, indicating that the change in association of myosin with the cytoskeleton is transmitted directly or indirectly by cyclic GMP. We measured the cyclic AMP-induced Ca2+ uptake with a Ca(2+)-sensitive electrode and found that Ca2+ uptake was prolonged in stmF mutants but not in the parental strain. The G alpha 2 mutant strain HC33 (fgdA), devoid of InsP3 release and receptor/guanylate cyclase coupling, lacked Ca2+ uptake. However, the latter response and cyclic GMP formation were normal in the signal-relay mutant strain agip 53 where cyclic AMP-stimulated cyclic AMP synthesis is absent. LiCl, which inhibits InsP3 formation in Dictyostelium, blocked Ca2+ uptake in a dose-dependent manner. The data indicate that the receptor-mediated Ca2+ uptake depends on the InsP3 pathway and is regulated by cyclic GMP. The rate of Ca2+ uptake was correlated in time with the association of myosin with the cytoskeleton, suggesting that Ca2+ uptake is involved in the motility response of the cells.

scholarly journals Fluoridation of the public water supply and prevalence of dental fluorosis in a peripheral district of the municipality of Bauru, SP

2006 ◽  
Vol 14 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Irene Ramires ◽  
Kelly Polido Kaneshiro Olympio ◽  
Andréa Gutierrez Maria ◽  
Juliano Pelim Pessan ◽  
Vanessa Eid Silva Cardoso ◽  
...  
Keyword(s):  
Water Supply ◽  
Water Samples ◽  
Dental Fluorosis ◽  
Fluoride Concentration ◽  
Epidemiological Survey ◽  
Public Water Supply ◽  
Prior Authorization ◽  
The Public ◽  
Electrode Method ◽  
Sensitive Electrode

OBJECTIVES: The objectives of this study were to assess the fluoride concentration in the public water supply and the prevalence of dental fluorosis in schoolchildren between 7 and 15 years old, living in a peripheral district of the municipality of Bauru. MATERIAL AND METHODS: For this, fifty two water samples were collected on three different days of one week. These samples were analyzed for fluoride by means of the ion-sensitive electrode method (Orion 9609) coupled to a potentiometer (Procyon, model 720). In this method, 1.0 mL of TISAB II (Orion) was added to 1.0 mL of the sample. For the epidemiological survey of fluorosis, 52 schoolchildren of both genders, aged between 7 and 15 were assessed, with prior authorization from their caretakers. Only one person examined the children, after supervised toothbrushing and drying with cotton wool rolls. The TF index was used. RESULTS: The fluoride concentrations in the water samples ranged from 0.62 to 1.20 mg/L, with a mean of 0.9 mg/L. The prevalence of dental fluorosis was 33%, with severity ranging from TF1 to TF4 (Kappa of 0.73 and concordance of 83.33%). CONCLUSIONS: The results from the analysis of water samples indicated a fluoride concentration greater than recommended for Bauru. The fluorosis levels found were higher than expected for a peripheral district, in which water is one of the few sources of fluoride.

Ionized Calcium Concentration Measured by a New Double-Membrane Calcium-Sensitive Electrode

1976 ◽  
pp. 491-493 ◽  
Author(s):  
S. Pors Nielsen ◽  
J. Trap-Jensen ◽  
O. Hartling ◽  
T. Falck Christiansen
Keyword(s):  
Calcium Concentration ◽  
Ionized Calcium ◽  
Double Membrane ◽  
Sensitive Electrode
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