Mapping strategy for resistance genes against Cladosporium fulvum on the short arm of Chromosome 1 of tomato: Cf-ECP5 near the Hcr9 Milky Way cluster

2000 ◽  
Vol 101 (4) ◽  
pp. 661-668 ◽  
Author(s):  
J. P. W. Haanstra ◽  
F. Meijer-Dekens ◽  
R. Laugé ◽  
D. C. Seetanah ◽  
M. H. A. J. Joosten ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Milky Way ◽  
Chromosome 1 ◽  
Cladosporium Fulvum ◽  
Mapping Strategy

The Solanum pimpinellifolium Cf-ECP1 and Cf-ECP4 genes for resistance to Cladosporium fulvum are located at the Milky Way locus on the short arm of chromosome 1

2007 ◽  
Vol 115 (8) ◽  
pp. 1127-1136 ◽  
Author(s):  
Eleni Soumpourou ◽  
Michael Iakovidis ◽  
Laetitia Chartrain ◽  
Verity Lyall ◽  
Colwyn M. Thomas
Keyword(s):  
Milky Way ◽  
Chromosome 1 ◽  
Cladosporium Fulvum ◽  
Solanum Pimpinellifolium

scholarly journals Homologues of the Cf-9 Disease Resistance Gene (Hcr9s) Are Present at Multiple Loci on the Short Arm of Tomato Chromosome 1

1999 ◽  
Vol 12 (2) ◽  
pp. 93-102 ◽  
Author(s):  
Martin Parniske ◽  
Brande B. H. Wulff ◽  
Guusje Bonnema ◽  
Colwyn M. Thomas ◽  
David A. Jones ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Milky Way ◽  
Chromosome 1 ◽  
Disease Resistance Gene ◽  
Cladosporium Fulvum ◽  
Large Gene ◽  
Multiple Loci ◽  
Sequence Homologies ◽  
Intergenic Regions ◽  
Complex Locus

The tomato Cf-4 and Cf-9 genes map at a genetically complex locus on the short arm of chromosome 1 and confer resistance against Cladosporium fulvum through recognition of different pathogen-encoded avirulence determinants. Cf-4 and Cf-9 are members of a large gene family (Hcr9s, Homologues of Cladosporium fulvum resistance gene Cf-9), some of which encode additional distinct recognition specificities. A genetic analysis of the majority of Hcr9s suggests that their distribution is spatially restricted to the short arm of chromosome 1. Two loci of clustered Hcr9 genes have been analyzed physically that mapped distal (Northern Lights) and proximal (Southern Cross) to the Cf-4/9 locus (Milky Way). Sequence homologies between intergenic regions at Southern Cross and Milky Way indicate local Hcr9 duplication preceded cluster multiplication. The multiplication of clusters involved DNA flanking Hcr9 sequences as indicated by conserved lipoxy-genase sequences at Southern Cross and Milky Way. The similar spatial distribution of Hcr9 clusters in different Lycopersicon spp. suggests Hcr9 cluster multiplication preceded speciation.

scholarly journals  Resistance of Arabidopsis thaliana to the obligate biotrophic parasite Plasmodiophora brassicae

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 519-522 ◽  
Author(s):  
A. Arbeiter ◽  
M. Fähling ◽  
H. Graf ◽  
M.D. Sacristán ◽  
J. Siemens
Keyword(s):  
Arabidopsis Thaliana ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Plasmodiophora Brassicae ◽  
Chromosome 1 ◽  
Coding Sequences ◽  
Resistance Phenotype ◽  
Resistance Reaction

Two resistance phenotypes to P. brassicae have been found in A. thaliana. A first resistance phenotype has been detected to the isolate 'e<sub>2</sub>' and is polygenically inherited. The second resistance to isolate 'e<sub>3</sub>' is caused by the dominant resistance gene RPB1. By crossing no influence could be shown for salicylic acid, jasmonic acid and ethylene in the latter resistance reaction. The RPB1 locus was narrowed down to 71 kb on chromosome 1, where three pseudogenes and 13 coding sequences are located. Six of them showed cosegregation with RPB1. None of these sequences have similarities to identified resistance genes or other known genes. Ten coding sequences were expressed, but CDS9 was exclusively expressed in the resistant ecotype Tsu-0.

RESISTANCE TO CLADOSPORIUM FULVUM CKE. OBTAINED FROM WILD SPECIES OF TOMATO

1964 ◽  
Vol 42 (11) ◽  
pp. 1541-1554 ◽  
Author(s):  
E. A. Kerr ◽  
D. L. Bailey
Keyword(s):  
Wild Species ◽  
Chromosome 1 ◽  
Cladosporium Fulvum ◽  
Breeding Lines ◽  
Genetic Bases

In the last 20 years several varieties and breeding lines of tomato have been developed which possess immunity from C. fulvum races 1 to 9. Vagabond, Vinequeen, and V548 obtained their immunity from L. hirsutum Humb. and Bonpl.; V501 from L. hirsutum var. glabralum Muller; V542, Vantage, Waltham Mold Proof Forcing No. 22, etc. from L. peruvianum (L.) Mill.; and V545 from L. pimpinellifolium (Jusl.) Mill. Studies are reported herein on the genetic bases of resistance in these varieties.Single genes for immunity from C. fulvum race 6 were isolated in these varieties and breeding lines. Crosses between the varieties indicated that the immunity from all of these sources is conditioned by the same gene or by genes that are very closely linked. Differential races must be found before this point can be determined with certainty. The gene from L. hirsutum which confers immunity to race 6 in Vinequeen is designated as Cf4. Cf4 has been shown to be located on chromosome 1, probably near Cf1.L. hirsutum, L. pimpinellifolium, and L. esculentum var. cerasiforme Mill. among them have several other genes conferring a lower level of resistance to race 6.

Molecular aspects of avirulence genes of the tomato pathogen Cladosporium fulvum

1995 ◽  
Vol 73 (S1) ◽  
pp. 490-494 ◽  
Author(s):  
Pierre J. G. M. de Wit ◽  
Matthieu H. A. J. Joosten ◽  
Guy Honée ◽  
Paul J. M. J. Vossen ◽  
Ton J. Cozijnsen ◽  
...  
Keyword(s):  
Host Plant ◽  
Resistance Genes ◽  
Hypersensitive Response ◽  
Fungal Pathogens ◽  
Direct Interaction ◽  
Defense Responses ◽  
Cladosporium Fulvum ◽  
Avirulence Genes ◽  
Host Genotype ◽  
Gene For Gene

Host genotype specificity in interactions between biotrophic fungal pathogens and plants in most cases complies with the gene-for-gene model. Success or failure of infection is determined by the absence or presence of complementary genes, avirulence and resistance genes, in the pathogen and the host plant, respectively. Resistance, expressed by the induction of a hypersensitive response followed by other defence responses in the host, is envisaged to be based on recognition of the pathogen, mediated through direct interaction between products of avirulence genes of the pathogen (the so-called race-specific elicitors) and receptors in the host plant, the putative products of resistance genes. The interaction between the biotrophic fungus Cladosporium fulvum and its only host, tomato, is a model system to study fungus–plant gene-for-gene relationships. Here we review research on isolation, characterization, and biological function of two race-specific elicitors AVR4 and AVR9 of C. fulvum and cloning and regulation of their encoding genes. Key words: avirulence genes, race-specific elicitors, resistance genes, hypersensitive response, host defense responses.

scholarly journals Apple Contains Receptor-like Genes Homologous to the Cladosporium fulvum Resistance Gene Family of Tomato with a Cluster of Genes Cosegregating with Vf Apple Scab Resistance

2001 ◽  
Vol 14 (4) ◽  
pp. 508-515 ◽  
Author(s):  
Boris A. Vinatzer ◽  
Andrea Patocchi ◽  
Luca Gianfranceschi ◽  
Stefano Tartarini ◽  
Hong-Bin Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Transmembrane Domain ◽  
Venturia Inaequalis ◽  
Scab Resistance ◽  
Common Disease ◽  
Resistance Locus ◽  
Cladosporium Fulvum ◽  
High Quality

Scab caused by the fungal pathogen Venturia inaequalis is the most common disease of cultivated apple (Malus × domestica Borkh.). Monogenic resistance against scab is found in some small-fruited wild Malus species and has been used in apple breeding for scab resistance. Vf resistance of Malus floribunda 821 is the most widely used scab resistance source. Because breeding a high-quality cultivar in perennial fruit trees takes dozens of years, cloning disease resistance genes and using them in the transformation of high-quality apple varieties would be advantageous. We report the identification of a cluster of receptor-like genes with homology to the Cladosporium fulvum (Cf) resistance gene family of tomato on bacterial artificial chromosome clones derived from the Vf scab resistance locus. Three members of the cluster were sequenced completely. Similar to the Cf gene family of tomato, the deduced amino acid sequences coded by these genes contain an extracellular leucine-rich repeat domain and a transmembrane domain. The transcription of three members of the cluster was determined by reverse transcription-polymerase chain reaction to be constitutive, and the transcription and translation start of one member was verified by 5′ rapid amplification of cDNA ends. We discuss the parallels between Cf resistance of tomato and Vf resistance of apple and the possibility that one of the members of the gene cluster is the Vf gene. Cf homologs from other regions of the apple genome also were identified and are likely to present other scab resistance genes.

scholarly journals Genes encoding recognition of the Cladosporium fulvum effector protein Ecp5 are encoded at several loci in the tomato genome

2019 ◽  
Author(s):  
Michail Iakovidis ◽  
Eleni Soumpourou ◽  
Elisabeth Anderson ◽  
Graham Etherington ◽  
Scott Yourstone ◽  
...  
Keyword(s):  
Hypersensitive Response ◽  
Milky Way ◽  
Effector Protein ◽  
Individual Plant ◽  
Cladosporium Fulvum ◽  
Chromosome 11 ◽  
Tomato Genome ◽  
Solanum Pimpinellifolium ◽  
Tomato Species ◽  
Genes Encoding

ABSTRACTThe molecular interactions between tomato and Cladosporium fulvum have been an important model for molecular plant pathology. Complex genetic loci on tomato chromosomes 1 and 6 harbor genes for resistance to Cladosporium fulvum, encoding receptor like-proteins that perceive distinct Cladosporium fulvum effectors and trigger plant defenses. Here, we report classical mapping strategies for loci in tomato accessions that respond to Cladosporium fulvum effector Ecp5, which is very sequence-monomorphic. We screened 139 wild tomato accessions for an Ecp5-induced hypersensitive response, and in five accessions, the Ecp5-induced hypersensitive response segregated as a monogenic trait, mapping to distinct loci in the tomato genome. We identified at least three loci on chromosomes 1, 7 and 12 that harbor distinct Cf-Ecp5 genes in four different accessions. Our mapping showed that the Cf-Ecp5 in Solanum pimpinellifolium G1.1161 is located at the Milky Way locus. The Cf-Ecp5 in Solanum pimpinellifolium LA0722 was mapped to the bottom arm of chromosome 7, while the Cf-Ecp5 genes in Solanum lycopersicum Ontario 7522 and Solanum pimpinellifolium LA2852 were mapped to the same locus on the top arm of chromosome 12. Bi-parental crosses between accessions carrying distinct Cf-Ecp5 genes revealed putative genetically unlinked suppressors of the Ecp5-induced hypersensitive response. Our mapping also showed that Cf-11 is located on chromosome 11, close to the Cf-3 locus. The Ecp5-induced hypersensitive response is widely distributed within tomato species and is variable in strength. This novel example of convergent evolution could be used for choosing different functional Cf-Ecp5 genes according to individual plant breeding needs.

Mapping Blast Resistance Genes in Rice Varieties ‘Minghui 63’ and ‘M-202’

Plant Disease ◽  
2021 ◽  
Author(s):  
Yulin Jia ◽  
Melissa H Jia ◽  
Zongbu Yan
Keyword(s):  
Resistance Genes ◽  
Blast Resistance ◽  
Interval Mapping ◽  
Blast Disease ◽  
Chromosome 1 ◽  
Rice Breeding ◽  
Resistance Qtls ◽  
Rice Varieties ◽  
Short Period ◽  
Blast Resistance Genes

Rice blast disease caused by the fungus Magnaporthe oryzae (syn. M. grisea) is one of the most lethal diseases for sustainable rice production worldwide. Blast resistance mediated by major resistance genes are often broken-down after a short period of deployment, while minor blast resistance genes, each providing a small effect on disease reactions, are more durable. In the present study, we first evaluated disease reactions of two rice breeding parents ‘Minghui 63’ and ‘M-202’ with 11 US blast races, IA45, IB1, IB45, IB49, IB54, IC1, IC17, ID1, IE1, IG1, and IH1 commonly found under greenhouse conditions using a category disease rating resembling infection types under field conditions. ‘Minghui 63’ exhibited differential resistance responses in comparison with that of ‘M-202’ to the tested blast races. A recombinant inbred line (RIL) population of 275 lines from a cross between ‘Minghui 63’ and ‘M-202’ was also evaluated with the above mentioned blast races. The population was genotyped with 156 simple sequence repeat (SSR) and insertion and deletion (Indel) markers. A linkage map with a genetic distance of 1022.84 cM was constructed using inclusive composite interval mapping (ICIM) software. A total of 10 resistance QTLs, eight from ‘Minghui 63’ and two from ‘M-202’, were identified. One major QTL, qBLAST2 on chr 2, was identified by seven races/isolates. The remaining nine minor resistance QTLs were mapped on chromosome 1, 3, 6, 9, 10, 11 and 12. These findings provide useful genetic markers and resources to tag minor blast resistance genes for marker assisted selection in rice breeding program and for further studies of underlying genes.

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