A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers

1998 ◽  
Vol 97 (7) ◽  
pp. 1083-1089 ◽  
Author(s):  
A. Spada ◽  
E. Caporali ◽  
G. Marziani ◽  
P. Portaluppi ◽  
F. M. Restivo ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Map ◽  
Asparagus Officinalis ◽  
Rflp Rapd

Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers

1993 ◽  
Vol 238-238 (1-2) ◽  
pp. 129-137 ◽  
Author(s):  
György B. Kiss ◽  
Gyula Csanádi ◽  
Katalin Kálmán ◽  
Péter Kaló ◽  
László Ökrész
Keyword(s):  
Genetic Map ◽  
Morphological Markers ◽  
Rflp Rapd

scholarly journals RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis

2012 ◽  
Vol 39 (No. 1) ◽  
pp. 33-37 ◽  
Author(s):  
Y. Ii ◽  
A. Uragami ◽  
Y. Uno ◽  
M. Kanechi ◽  
N. Inagaki
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Genomic Dna ◽  
Asparagus Officinalis ◽  
Breeding Line ◽  
Male And Female ◽  
Future Studies ◽  
Random Primers

Asparagus (Asparagus officinalis L.) plants are dioecious. All-male cultivars are desired because of their higher yields. To increase the proportion of male individuals planted in the field and expedite the breeding of all-male cultivars in asparagus, development of generally applicable molecular markers to distinguish male and female individuals is required. Bulked genomic DNA samples from ten male (XY) and ten female (XX) plants was screened with 10-bp random primers. Of the 188 primers tested, the primer T35R54 produced a 1600-bp fragment observed only in male individuals. The specificity of this T35R54-1600 marker was verified using DNA from one supermale (YY) and one female (XX) breeding line and their four F<sub>1</sub> progenies (XY). The T35R54-1600 marker fragment was observed in both supermale and all-male lines. The sequence of the T35R54 primer (5'-TTCACGGTGG-3') was absent among the sequences of primers or amplified fragments from previous studies. Therefore, this marker could be useful as a sex-related marker in future studies to increase the reliability of sex determination in asparagus.

TRAP molecular markers as a system for saturation of the genetic map of durum wheat

Euphytica ◽  
2013 ◽  
Vol 194 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Virginia Menzo ◽  
Angelica Giancaspro ◽  
Stefania Giove ◽  
Domenica Nigro ◽  
Silvana Zacheo ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Durum Wheat ◽  
Genetic Map

scholarly journals Genetic Map of Cotton with Molecular Markers

2015 ◽  
Vol 06 (02) ◽  
pp. 289-297 ◽  
Author(s):  
Camila Campêlo de Sousa ◽  
Lívio Carvalho de Figueirêdo ◽  
Maria das Graças Medina Arrais ◽  
Sérgio Emílio dos Santos Valente
Keyword(s):  
Molecular Markers ◽  
Genetic Map

scholarly journals Molecular Markers and Germplasm Enhancement: A Case Study in Tomato

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 693a-693
Author(s):  
V. Meglic ◽  
R.T. Chetelat
Keyword(s):  
Molecular Markers ◽  
Species Group ◽  
Wild Relatives ◽  
Linkage Maps ◽  
Germplasm Enhancement ◽  
Solanum Lycopersicoides ◽  
Genetic Stocks ◽  
Rflp Rapd ◽  
Selection Of

The C.M. Rick Tomato Genetic Resources Center (TGRC) is a genebank of wild relatives, monogenic mutants, and miscellaneous genetic stocks of tomato. The wild species group includes representatives of all nine Lycopersicon spp., as well as four related Solanum species. One of the roles of the TGRC has been to foster the use of the widest available gene pool for tomato researchers. The wild nightshade Solanum lycopersicoides possesses a number of potentially useful traits, but has been untapped by breeders because of sterility and incompatibility barriers. We are using molecular markers to identify alien chromosomal segments introgressed from S. lycopersicoides into tomato. This project involves development of RFLP, RAPD, and isozyme marker linkage maps and their use in selection of homozygous segmental substitutions in backcross inbred progenies. In this fashion, a large proportion of the S. lycopersicoides genome has been integrated into the cultivated tomato. This study has also provided information on the nature of sterility and novel variation in hybrid derivatives.

scholarly journals Genetic mapping studies in Coffea sp using molecular marker methods Studi peta genetik pada Coffea sp. menggunakan metode penanda molekuler

2016 ◽  
Vol 83 (2) ◽  
Author(s):  
. PRIYONO ◽  
Riza Arief PUTRANTO
Keyword(s):  
Molecular Markers ◽  
Genetic Mapping ◽  
Genetic Map ◽  
Crop Improvement ◽  
Average Density ◽  
Genetic Maps ◽  
Gene Characterization ◽  
Robusta Coffee ◽  
Dna And Rna ◽  
Genomic Comparative Analysis

AbstrakAnalisis genetik telah  menjadi alat yang penting  dalam  pemuliaan  tanaman untuk perbaikan sifat penting tanaman. Salah satu potensi terbesar dari analisis tersebut adalah identifikasi penanda molekuler yang berguna untuk pemetaan genetik. Pemetaan genetik  merupakan  salah satu langkah penting dari analisis  genetik.  Intisari  dari   semua pemetaan genetik adalah  menempatkan  koleksi  pe- nanda molekuler pada posisi tertentu dalam genom. Hal tersebut dapat kemudian digunakan untuk meng- identifikasi lokus sifat kuantitatif (QTLs) dengan memanfaatan keragaman genetik alami yang tersedia dan meningkatkan sifat-sifat penting serta berharga. Sampai saat ini, tiga belas peta genetik telah dipublikasi dan tersedia pada Coffea sp. yang menciptakan database besar untuk kerangka genetik. Sebuah peta genetik terbaru dengan akses terbuka dan berfungsi sebagai referensi telah dibangun oleh International Coffee Genomics Network (ICGN). Peta tersebut tediri dari 3230 lokus, dengan panjang peta 1471 cM (1cm ~ 500 Kb) serta kepadatan satu penanda setiap 220 Kb. Peta-peta genetik pada tanaman kopi telah digunakan dari karakterisasi gen hingga analisis komparatif genom dengan spesies tanaman yang berbeda. Saat ini, pesatnya kemajuan teknologi New Genome Sequencing (NGS) untuk sekuensing DNA dan RNA memungkinkan validasi dari peta-peta genetik untuk prediksi QTLs serta gen-gen yang membawa sifat penting Coffea sp.AbstractGenetic analysis has become an important tool in plant breeding for crop improvement. One of their greatest potential appears to be the identification of molecular markers useful for genetic mapping. Genetic mapping is one of important steps in genetic analysis. The essence of all genetic mapping is to place a collection of molecular markers onto their respective positions on the genome. Thus, it leads to identification of new quantitative trait loci (QTLs) by making benefits of natural available genetic diversity.and to improve important and valuable traits. Until present, thirteen genetic maps were published and available in Coffea sp. creating a huge database for genetic framework. One most recent and open reference genetic map for robusta coffee has been generated by the International Coffee Genomics Network (ICGN) comprising 3230 loci, genetic size 1471 cM (1cM ~500 Kb), with an average density close to one marker every 220 Kb. The Coffea genetic maps have been utilized from gene characterization to genomic comparative analysis with different plant species. Nowadays, the feasibility of NGS for DNA and RNA sequencing allow the validation of genetic map related to the prediction of QTLs and adjacent genes related to important traits for Coffea sp. 

scholarly journals A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

2007 ◽  
Vol 20 (2) ◽  
pp. 153-166 ◽  
Author(s):  
Juliana Stein ◽  
Silvina C. Pessino ◽  
Eric J. Martínez ◽  
María Pía Rodriguez ◽  
Lorena A. Siena ◽  
...  
Keyword(s):  
Single Dose ◽  
Molecular Markers ◽  
Genetic Map ◽  
Paspalum Notatum

Construction of a reference linkage map for melon

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 836-845 ◽  
Author(s):  
M Oliver ◽  
J Garcia-Mas ◽  
M Cardús ◽  
N Pueyo ◽  
A I López-Sesé ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Linkage Map ◽  
Genetic Map ◽  
Cucumis Melo ◽  
Morphological Trait ◽  
Linkage Groups ◽  
Resistant Gene ◽  
Anchor Points ◽  
Cucumis Melo L

A map of melon (Cucumis melo L.) with 411 markers (234 RFLPs, 94 AFLPs, 47 RAPDs, 29 SSRs, five inter-SSRs, and two isozymes) and one morphological trait (carpel number) was constructed using the F2 progeny of a cross between the Korean accession PI161375 and the Spanish melon type 'Pinyonet Piel de Sapo'. RFLPs were obtained using 212 probes from different genomic and cDNA melon libraries, including 16 Arabidopsis ESTs, 13 Cucumis known genes, and three resistant gene homologues. Most loci (391) mapped to 12 major linkage groups, spanning a total genetic distance of 1197 cM, with an average map interval of 3 cM/marker. The remaining 21 loci (six RAPDs and 15 AFLPs) were not linked. A majority (66%) of the markers were codominant (RFLPs, SSRs, and isozymes), making them easily transferable to other melon crosses. Such markers can be used as a reference, to merge other melon and cucumber maps already constructed. Indeed, some of them (23 SSRs, 14 RFLPs, one isozyme, and one morphological trait) could act as anchor points with other published cucurbit maps.Key words: Cucumis melo, genetic map, molecular markers, RFLPs, SSRs.

Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques

1996 ◽  
Vol 14 (2) ◽  
pp. 156-169 ◽  
Author(s):  
Jhy-Jhu Lin ◽  
Jonathan Kuo ◽  
Jin Ma ◽  
James A. Saunders ◽  
Hunter S. Beard ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Dna Mapping ◽  
Mapping Techniques ◽  
Rflp Rapd
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