Construction of a reference linkage map for melon

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 836-845 ◽  
Author(s):  
M Oliver ◽  
J Garcia-Mas ◽  
M Cardús ◽  
N Pueyo ◽  
A I López-Sesé ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Linkage Map ◽  
Genetic Map ◽  
Cucumis Melo ◽  
Morphological Trait ◽  
Linkage Groups ◽  
Resistant Gene ◽  
Anchor Points ◽  
Cucumis Melo L

A map of melon (Cucumis melo L.) with 411 markers (234 RFLPs, 94 AFLPs, 47 RAPDs, 29 SSRs, five inter-SSRs, and two isozymes) and one morphological trait (carpel number) was constructed using the F2 progeny of a cross between the Korean accession PI161375 and the Spanish melon type 'Pinyonet Piel de Sapo'. RFLPs were obtained using 212 probes from different genomic and cDNA melon libraries, including 16 Arabidopsis ESTs, 13 Cucumis known genes, and three resistant gene homologues. Most loci (391) mapped to 12 major linkage groups, spanning a total genetic distance of 1197 cM, with an average map interval of 3 cM/marker. The remaining 21 loci (six RAPDs and 15 AFLPs) were not linked. A majority (66%) of the markers were codominant (RFLPs, SSRs, and isozymes), making them easily transferable to other melon crosses. Such markers can be used as a reference, to merge other melon and cucumber maps already constructed. Indeed, some of them (23 SSRs, 14 RFLPs, one isozyme, and one morphological trait) could act as anchor points with other published cucurbit maps.Key words: Cucumis melo, genetic map, molecular markers, RFLPs, SSRs.

scholarly journals A Dense Genetic Map of the Silkworm, Bombyx mori, Covering All Chromosomes Based on 1018 Molecular Markers

Genetics ◽  
1998 ◽  
Vol 150 (4) ◽  
pp. 1513-1525 ◽  
Author(s):  
Yuji Yasukochi
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Markers ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Genetic Markers ◽  
Genetic Map ◽  
Z Chromosome ◽  
Linkage Groups ◽  
Average Interval ◽  
Silkworm Bombyx Mori

Abstract A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome. Most of the markers, covering ∼2000 cM, were randomly amplified polymorphic DNAs amplified with primer-pairs in combinations of 140 commercially available decanucleotides. In addition, eight known genes and five visible mutations were mapped. Bombyx homologues of engrailed and invected genes were found to be closely linked, as in Drosophila melanogaster. The average interval between markers was ∼2 cM, equal to ∼500 kb. The correspondence of seven linkage groups to counterparts of the conventional linkage map was determined. This map is the first linkage map in insects having a large number of chromosomes (n = 28) that covers all chromosomes without any gaps.

scholarly journals A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)

2011 ◽  
Vol 11 (1) ◽  
pp. 111 ◽  
Author(s):  
Aurora Diaz ◽  
Mohamed Fergany ◽  
Gelsomina Formisano ◽  
Peio Ziarsolo ◽  
José Blanca ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Quantitative Trait ◽  
Cucumis Melo ◽  
Trait Loci ◽  
Cucumis Melo L

scholarly journals An enhanced molecular marker based genetic map of perennial ryegrass (Lolium perenne) reveals comparative relationships with other Poaceae genomes

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 282-295 ◽  
Author(s):  
Elizabeth S Jones ◽  
Natalia L Mahoney ◽  
Michael D Hayward ◽  
Ian P Armstead ◽  
J Gilbert Jones ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Linkage Map ◽  
Genetic Map ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Genetic Linkage Map ◽  
Population Based ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Integrated Genetic Map

A molecular-marker linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross population based on the mating of a multiple heterozygous individual with a doubled haploid genotype. RFLP, AFLP, isoenzyme, and EST data from four collaborating laboratories within the International Lolium Genome Initiative were combined to produce an integrated genetic map containing 240 loci covering 811 cM on seven linkage groups. The map contained 124 codominant markers, of which 109 were heterologous anchor RFLP probes from wheat, barley, oat, and rice, allowing comparative relationships between perennial ryegrass and other Poaceae species to be inferred. The genetic maps of perennial ryegrass and the Triticeae cereals are highly conserved in terms of synteny and colinearity. This observation was supported by the general agreement of the syntenic relationships between perennial ryegrass, oat, and rice and those between the Triticeae and these species. A lower level of synteny and colinearity was observed between perennial ryegrass and oat compared with the Triticeae, despite the closer taxonomic affinity between these species. It is proposed that the linkage groups of perennial ryegrass be numbered in accordance with these syntenic relationships, to correspond to the homoeologous groups of the Triticeae cereals.Key words: Lolium perenne, genetic linkage map, RFLP, AFLP, conserved synteny.

scholarly journals Centromere-Linkage Analysis and Consolidation of the Zebrafish Genetic Map

Genetics ◽  
1996 ◽  
Vol 142 (4) ◽  
pp. 1277-1288
Author(s):  
Stephen L Johnson ◽  
Michael A Gates ◽  
Michele Johnson ◽  
William S Talbot ◽  
Sally Horne ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Analysis ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Rapid Method ◽  
Genetic Map ◽  
Dna Polymorphisms ◽  
Linkage Groups

Abstract The ease of isolating mutations in zebrafish will contribute to an understanding of a variety of processes common to all vertebrates. To facilitate genetic analysis of such mutations, we have identified DNA polymorphisms closely linked to each of the 25 centromeres of zebrafish, placed centromeres on the linkage map, increased the number of mapped PCR-based markers to 652, and consolidated the number of linkage groups to the number of chromosomes. This work makes possible centromere-linkage analysis, a novel, rapid method to assign mutations to a specific linkage group using half-tetrads.

A peach linkage map integrating RFLPs, SSRs, RAPDs, and morphological markers

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Maria Teresa Dettori ◽  
Roberta Quarta ◽  
Ignazio Verde
Keyword(s):  
Molecular Markers ◽  
Comparative Analysis ◽  
Linkage Map ◽  
Morphological Traits ◽  
Prunus Persica ◽  
Average Distance ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Peach Genome ◽  
The Common

A linkage map was obtained using a BC1 progeny (Prunus persica × (P. persica × P. ferganensis)). The map is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 linkage groups. Loci, segregating in five different ratios, were integrated in the map with JoinMap 2.0 software. The map covers 521 cM of the peach genome. The average distance between adjacent loci is 4.8 cM. Two monogenic traits, flesh adhesion (F/f) and leaf glands (E/e), were placed on the map. Thirty-two loci in common with a saturated linkage map of Prunus allowed a comparative analysis to be made between the two maps. Homologies were found among the respective linkage groups. No relevant differences were observed in the linear order of the common loci.Key words: peach, linkage map, Prunus persica, Prunus ferganensis, molecular markers.

Integration of the Cytogenetic and Genetic Linkage Maps of Brassica oleracea

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1225-1234 ◽  
Author(s):  
Elaine C Howell ◽  
Guy C Barker ◽  
Gareth H Jones ◽  
Michael J Kearsey ◽  
Graham J King ◽  
...  
Keyword(s):  
Linkage Map ◽  
Brassica Oleracea ◽  
Genetic Map ◽  
Genetic Linkage ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Opposite Orientation ◽  
Cytogenetic Map ◽  
Specific Pcr ◽  
Genetic Linkage Maps

Abstract We have assigned all nine linkage groups of a Brassica oleracea genetic map to each of the nine chromosomes of the karyotype derived from mitotic metaphase spreads of the B. oleracea var. alboglabra line A12DHd using FISH. The majority of probes were BACs, with A12DHd DNA inserts, which give clear, reliable FISH signals. We have added nine markers to the existing integrated linkage map, distributed over six linkage groups. BACs were definitively assigned to linkage map positions through development of locus-specific PCR assays. Integration of the cytogenetic and genetic linkage maps was achieved with 22 probes representing 19 loci. Four chromosomes (2, 4, 7, and 9) are in the same orientation as their respective linkage groups (O4, O7, O8, and O6) whereas four chromosomes (1, 3, 5, and 8) and linkage groups (O3, O9, O2, and O1) are in the opposite orientation. The remaining chromosome (6) is probably in the opposite orientation. The cytogenetic map is an important resource for locating probes with unknown genetic map positions and is also being used to analyze the relationships between genetic and cytogenetic maps.

Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n = 28)

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 626-629 ◽  
Author(s):  
Yuji Yasukochi ◽  
Yutaka Banno ◽  
Kohji Yamamoto ◽  
Marian R Goldsmith ◽  
Hiroshi Fujii
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Linkage Analysis ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Linkage Maps ◽  
Wild Type ◽  
Linkage Groups ◽  
Sequence Tagged Sites ◽  
Silkworm Bombyx Mori

Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was carried out using BF1 progeny between wild-type and mutant stocks carrying morphological phenotypic markers. As a result, the counterparts for 26 of 28 molecular LGs were identified with their counterparts of the classical LGs. Two visible markers, Sel and Xan, representing different classical LGs, were found to be linked.Key words: Bombyx mori, classical linkage group (LG), PCR-based genotyping, mutant, STS.

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