Selection of stable Brassica napus-Brassica juncea recombinant lines resistant to blackleg (Leptosphaeria maculans). 2. A ‘to and fro’ strategy to localise and characterise interspecific introgressions on the B. napus genome

1998 ◽  
Vol 96 (8) ◽  
pp. 1097-1103 ◽  
Author(s):  
P. Barret ◽  
J. Guérif ◽  
J. P. Reynoird ◽  
R. Delourme ◽  
F. Eber ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Juncea ◽  
Leptosphaeria Maculans ◽  
Selection Of

Comparison of inoculation methods for selection of plants resistant to Leptosphaeria maculans in Brassica napus

1993 ◽  
Vol 73 (4) ◽  
pp. 1199-1207 ◽  
Author(s):  
W. M. McNabb ◽  
C. G. J. van den Berg ◽  
S. R. Rimmer
Keyword(s):  
Brassica Napus ◽  
Key Words ◽  
Correlation Coefficient ◽  
Leptosphaeria Maculans ◽  
Inoculation Methods ◽  
Inoculation Techniques ◽  
Large Populations ◽  
Disease Rating ◽  
The Relationship ◽  
Selection Of

The relationship between ratings following four inoculation techniques and field ratings and the usefulness of these techniques for screening large populations was determined. Plants of Brassica napus ’Westar,’ ’Wesroona’, ’Topas’, ’Hanna’ and ’R8314’ were inoculated with L. maculans using four techniques: cotyledon inoculation with cotyledon rating, leaf inoculation with leaf and stem rating, stem inoculation with stem rating and inoculation using infested oat kernels with stem rating. Plants from each combination of cultivar and technique were self-pollinated for evaluation of disease rating in the field. The highest precision was obtained with cotyledon rating following cotyledon inoculation. The correlation coefficient between the rating of inoculated plants and the field rating of their progeny ranged from 0.50** for oat kernel inoculation to 0.72** for cotyledon inoculation. Considerable variation for field rating was associated with each rating following artificial inoculation. Selection of resistant plants using low cotyledon reaction was a more efficient technique than leaf or stem inoculation; it was rapid, required less labour and space and correlated well with the field ratings. Key words: Leptosphaeria maculans, Brassica napus, blackleg, oilseed rape, inoculation methods, canola

scholarly journals Recent Findings Unravel Genes and Genetic Factors Underlying Leptosphaeria maculans Resistance in Brassica napus and Its Relatives

2020 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
Aldrin Y. Cantila ◽  
Nur Shuhadah Mohd Saad ◽  
Junrey C. Amas ◽  
David Edwards ◽  
Jacqueline Batley
Keyword(s):  
Brassica Napus ◽  
Genetic Factors ◽  
Quantitative Resistance ◽  
Genetic Resistance ◽  
Leptosphaeria Maculans ◽  
Gene Interaction ◽  
R Gene ◽  
R Genes ◽  
Blackleg Resistance ◽  
Avr Gene

Among the Brassica oilseeds, canola (Brassica napus) is the most economically significant globally. However, its production can be limited by blackleg disease, caused by the fungal pathogen Lepstosphaeria maculans. The deployment of resistance genes has been implemented as one of the key strategies to manage the disease. Genetic resistance against blackleg comes in two forms: qualitative resistance, controlled by a single, major resistance gene (R gene), and quantitative resistance (QR), controlled by numerous, small effect loci. R-gene-mediated blackleg resistance has been extensively studied, wherein several genomic regions harbouring R genes against L. maculans have been identified and three of these genes were cloned. These studies advance our understanding of the mechanism of R gene and pathogen avirulence (Avr) gene interaction. Notably, these studies revealed a more complex interaction than originally thought. Advances in genomics help unravel these complexities, providing insights into the genes and genetic factors towards improving blackleg resistance. Here, we aim to discuss the existing R-gene-mediated resistance, make a summary of candidate R genes against the disease, and emphasise the role of players involved in the pathogenicity and resistance. The comprehensive result will allow breeders to improve resistance to L. maculans, thereby increasing yield.

Development of an agronomically superior blackleg resistant canola cultivar in Brassica napus L. using doubled haploidy

1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Leptosphaeria Maculans ◽  
Resistance Breeding ◽  
Breeding Method ◽  
Brassica Napus L ◽  
Breeding Lines ◽  
University Of Alberta ◽  
Blackleg Resistance ◽  
The University

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus

Defoliation of Brassica napus increases severity of blackleg caused by Leptosphaeria maculans: implications for dual-purpose cropping

2010 ◽  
Vol 157 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Susan J. Sprague ◽  
John A. Kirkegaard ◽  
Stephen J. Marcroft ◽  
John M. Graham
Keyword(s):  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Dual Purpose

Digestibility energy and amino acids of canola meal from two species (Brassica juncea and Brassica napus) fed to distal ileum cannulated grower pigs1

2012 ◽  
Vol 90 (suppl_4) ◽  
pp. 218-220 ◽  
Author(s):  
M. H. A. Le ◽  
A. D. G. Buchet ◽  
E. Beltranena ◽  
W. J. J. Gerrits ◽  
R. T. Zijlstra
Keyword(s):  
Amino Acids ◽  
Brassica Napus ◽  
Brassica Juncea ◽  
Distal Ileum ◽  
Canola Meal

scholarly journals MORPHOLOGICAL CHARACTERISTICS OF BEE POLLEN OBTAINED FROM BRASSICA NAPUS L.

2016 ◽  
Vol 18 (2) ◽  
pp. 73-78
Author(s):  
N.M. Redina ◽  
L.O. Adamchuk ◽  
N.V. Nikolaieva ◽  
J. Brindza
Keyword(s):  
Brassica Napus ◽  
Local Population ◽  
Morphological Characteristics ◽  
Morphometric Measurements ◽  
Brassica Napus L ◽  
Pollen Collection ◽  
Bee Pollen ◽  
Total Pollen ◽  
Protein Feed ◽  
Selection Of

The purpose of this work was to research the morphological characteristics of monofloral bee pollen obtained from Brassica napus L. The formulated goals were: determine the period of bee pollen receiving; implement the selection of bee pollen samples during blooming period of species; establish the monoflorality ratio of the total pollen collection; explore the morphometric indicators of bee pollen by the parameters of pollen lump, shaping level and weight. Bee pollen collection was carried out in Skvyra district of Kyiv region from local population of Apis mellifera L. families. Morphometric measurements were performed in the Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra. Here were determined the amount of bee pollen per one day brought by one family: from  27.04 to 03.05.16 is 50.8 ± 2.25 g; from 04.05 to 06.05.16 – 100.0 ± 3.33 g; from 07.05 to 17.05.16 – 270.7 ± 11.79 g; from 18.05 to 22.05.16 – 100.5 ± 3.028 g; from 23.05 – 25.05.16 – 39.8 ± 2.74 g. Here were defined the morphological parameters of bee pollen lump from B. napus: length is in the range from 3.40 ± 0.061 mm to 3.66 ± 0.048 mm; width is in the range from 2.88 ± 0.059 mm to 3.26 ± 0.067 mm; weight is in the range from 8.65 ± 0.317 mg to 11.31 ± 0.241 mg; shaping level of bee pollen is in the range 4.62 – 4.96 points. The total monoflorality ratio constitutes from 79.94 ± 0.619 % to 98.00 ± 0.202 %. The obtained results confirmed that the activity of bee flying depends on the blooming period and intensity of the nectar secretion, on the collection of protein feed from B. napus. Pollen lumps from Brassica napus L. bee pollen were large, dense, regular shape without splits, which specified the possibility of its use for the producing on a commercial scale.  

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