Selection of stable Brassica napus-B. juncea recombinant lines resistant to blackleg (Leptosphaeria maculans). 1. Identification of molecular markers, chromosomal and genomic origin of the introgression

1997 ◽  
Vol 95 (7) ◽  
pp. 1104-1111 ◽  
Author(s):  
A. M. Chèvre ◽  
P. Barret ◽  
F. Eber ◽  
P. Dupuy ◽  
H. Brun ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Selection Of

scholarly journals Genetic Analysis of a Horizontal Resistance Locus BLMR2 in Brassica napus

2021 ◽  
Vol 12 ◽  
Author(s):  
Qiang Zhang ◽  
Hanna Dandena ◽  
Madison McCausland ◽  
Huizhi Liu ◽  
Zheng Liu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Chromosome Region ◽  
Leptosphaeria Maculans ◽  
Snp Markers ◽  
Resistance Locus ◽  
Horizontal Resistance ◽  
Blackleg Disease ◽  
Single Nucleotide ◽  
Sequence Characterized Amplified Regions

Leptosphaeria maculans causes blackleg disease in Brassica napus. The blackleg disease is mainly controlled by resistance genes in B. napus. Previous studies have shown that the blackleg resistant BLMR2 locus that conferred horizontal resistance under field conditions, is located on chromosome A10 of B. napus. The purpose of this study is to fine map this locus and hence identify a candidate gene underlying horizontal resistance. The spectrum of resistance to L. maculans isolates of the resistance locus BLMR2 was analyzed using near isogenic lines, resistant, and susceptible cultivars. The results showed that this locus was horizontally resistant to all isolates tested. Sequence characterized amplified regions (SCAR), simple sequence repeats (SSR), and single nucleotide polymorphism (SNP) markers were developed in the chromosome region of BLMR2 and a fine genetic map was constructed. Two molecular markers narrowed BLMR2 in a 53.37 kb region where six genes were annotated. Among the six annotated genes, BnaA10g11280D/BnaA10g11290D encoding a cytochrome P450 protein were predicted as the candidate of BLMR2. Based on the profiling of pathogen induced transcriptome, three expressed genes in the six annotated genes were identified while only cytochrome P450 showed upregulation. The candidate corresponds to the gene involved in the indole glucosinolate biosynthesis pathway and plant basal defense in Arabidopsis thaliana. The molecular markers identified in this study will allow the quick incorporation of the BLMR2 allele in rapeseed cultivars to enhance blackleg resistance.

Comparison of inoculation methods for selection of plants resistant to Leptosphaeria maculans in Brassica napus

1993 ◽  
Vol 73 (4) ◽  
pp. 1199-1207 ◽  
Author(s):  
W. M. McNabb ◽  
C. G. J. van den Berg ◽  
S. R. Rimmer
Keyword(s):  
Brassica Napus ◽  
Key Words ◽  
Correlation Coefficient ◽  
Leptosphaeria Maculans ◽  
Inoculation Methods ◽  
Inoculation Techniques ◽  
Large Populations ◽  
Disease Rating ◽  
The Relationship ◽  
Selection Of

The relationship between ratings following four inoculation techniques and field ratings and the usefulness of these techniques for screening large populations was determined. Plants of Brassica napus ’Westar,’ ’Wesroona’, ’Topas’, ’Hanna’ and ’R8314’ were inoculated with L. maculans using four techniques: cotyledon inoculation with cotyledon rating, leaf inoculation with leaf and stem rating, stem inoculation with stem rating and inoculation using infested oat kernels with stem rating. Plants from each combination of cultivar and technique were self-pollinated for evaluation of disease rating in the field. The highest precision was obtained with cotyledon rating following cotyledon inoculation. The correlation coefficient between the rating of inoculated plants and the field rating of their progeny ranged from 0.50** for oat kernel inoculation to 0.72** for cotyledon inoculation. Considerable variation for field rating was associated with each rating following artificial inoculation. Selection of resistant plants using low cotyledon reaction was a more efficient technique than leaf or stem inoculation; it was rapid, required less labour and space and correlated well with the field ratings. Key words: Leptosphaeria maculans, Brassica napus, blackleg, oilseed rape, inoculation methods, canola

scholarly journals Recent Findings Unravel Genes and Genetic Factors Underlying Leptosphaeria maculans Resistance in Brassica napus and Its Relatives

2020 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
Aldrin Y. Cantila ◽  
Nur Shuhadah Mohd Saad ◽  
Junrey C. Amas ◽  
David Edwards ◽  
Jacqueline Batley
Keyword(s):  
Brassica Napus ◽  
Genetic Factors ◽  
Quantitative Resistance ◽  
Genetic Resistance ◽  
Leptosphaeria Maculans ◽  
Gene Interaction ◽  
R Gene ◽  
R Genes ◽  
Blackleg Resistance ◽  
Avr Gene

Among the Brassica oilseeds, canola (Brassica napus) is the most economically significant globally. However, its production can be limited by blackleg disease, caused by the fungal pathogen Lepstosphaeria maculans. The deployment of resistance genes has been implemented as one of the key strategies to manage the disease. Genetic resistance against blackleg comes in two forms: qualitative resistance, controlled by a single, major resistance gene (R gene), and quantitative resistance (QR), controlled by numerous, small effect loci. R-gene-mediated blackleg resistance has been extensively studied, wherein several genomic regions harbouring R genes against L. maculans have been identified and three of these genes were cloned. These studies advance our understanding of the mechanism of R gene and pathogen avirulence (Avr) gene interaction. Notably, these studies revealed a more complex interaction than originally thought. Advances in genomics help unravel these complexities, providing insights into the genes and genetic factors towards improving blackleg resistance. Here, we aim to discuss the existing R-gene-mediated resistance, make a summary of candidate R genes against the disease, and emphasise the role of players involved in the pathogenicity and resistance. The comprehensive result will allow breeders to improve resistance to L. maculans, thereby increasing yield.

Development of an agronomically superior blackleg resistant canola cultivar in Brassica napus L. using doubled haploidy

1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Leptosphaeria Maculans ◽  
Resistance Breeding ◽  
Breeding Method ◽  
Brassica Napus L ◽  
Breeding Lines ◽  
University Of Alberta ◽  
Blackleg Resistance ◽  
The University

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus

scholarly journals Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype

2014 ◽  
Vol 37 (3) ◽  
pp. 556-559 ◽  
Author(s):  
Lenka Havlícková ◽  
Eva Jozová ◽  
Miroslav Klíma ◽  
Vratislav Kucera ◽  
Vladislav Curn
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Class I ◽  
Brassica Napus L ◽  
S Haplotype
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