Time course of ultrastructural changes and immunoelectron microscopic localization of neurocalcin in motor endplates of the lumbrical muscles of rats given a single administration of 2,5-di(tert-butyl)-1,4-hydroquinone

2000 ◽  
Vol 99 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Takayoshi Imazawa ◽  
Kunitoshi Mitsumori ◽  
Satoshi Kitajima ◽  
Hiroshi Onodera ◽  
Toru Tamura ◽  
...  
Keyword(s):  
Time Course ◽  
Ultrastructural Changes ◽  
Single Administration ◽  
Motor Endplates ◽  
Tert Butyl ◽  
Microscopic Localization ◽  
Lumbrical Muscles

Ultrastructural changes in motor endplates of the lumbrical muscles of rats induced by a microsomal Ca 2+ ATPase inhibitor, 2,5-di(tert-butyl)-1,4-hydroquinone

1997 ◽  
Vol 72 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Kunitoshi Mitsumori ◽  
Takayoshi Imazawa ◽  
Hiroshi Onodera ◽  
Michihito Takahashi ◽  
Satoshi Kitajima ◽  
...  
Keyword(s):  
Ultrastructural Changes ◽  
Atpase Inhibitor ◽  
Motor Endplates ◽  
Tert Butyl ◽  
Lumbrical Muscles

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

scholarly journals Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

2021 ◽  
Vol 22 (2) ◽  
pp. 811
Author(s):  
Huifang Yan ◽  
Peisheng Mao
Keyword(s):  
Antioxidant Capacity ◽  
Avena Sativa ◽  
Proteomic Analysis ◽  
Time Course ◽  
Intact Cell ◽  
Membrane Integrity ◽  
Acid Synthesis ◽  
Ultrastructural Changes ◽  
Oat Seeds ◽  
Aged Seeds

Melatonin priming is an effective strategy to improve the germination of aged oat (Avena sativa L.) seeds, but the mechanism involved in its time-course responses has remained largely unknown. In the present study, the phenotypic differences, ultrastructural changes, physiological characteristics, and proteomic profiles were examined in aged and melatonin-primed seed (with 10 μM melatonin treatment for 12, 24, and 36 h). Thus, 36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings, relatively intact cell ultrastructures, and enhanced antioxidant capacity. Proteomic analysis revealed 201 differentially abundant proteins between aged and T36 seeds, of which 96 were up-accumulated. In melatonin-primed seeds, the restoration of membrane integrity by improved antioxidant capacity, which was affected by the stimulation of jasmonic acid synthesis via up-accumulation of 12-oxo-phytodienoic acid reductase, might be a candidate mechanism. Moreover, the relatively intact ultrastructures enabled amino acid metabolism and phenylpropanoid biosynthesis, which were closely associated with energy generation through intermediates of pyruvate, phosphoenolpyruvate, fumarate, and α-ketoglutarate, thus providing energy, active amino acids, and secondary metabolites necessary for germination improvement of aged seeds. These findings clarify the time-course related pathways associated with melatonin priming on promoting the germination of aged oat seeds.

Properties of spontaneous potentials at denervated motor endplates of the frog

1976 ◽  
Vol 194 (1115) ◽  
pp. 195-210 ◽  
Keyword(s):  
Schwann Cell ◽  
Time Course ◽  
Experimental Treatment ◽  
Spider Venom ◽  
Black Widow ◽  
Motor Endplates ◽  
Black Widow Spider ◽  
Ringer’S Solution ◽  
Black Widow Spider Venom ◽  
Widow Spider

Some properties of spontaneous miniature potentials at denervated frog motor endplates (Schwann cell-min. e. p. ps) have been investigated. Schwann cell-min. e. p. ps showed a slower and more variable time course than min. e. p. ps at inneryated endplates. The occurrence of Schwann cell-min. e. p. ps usually obeyed Poisson statistics and in the absence of experimental treatment their mean frequency remained stable for several hours. Schwann cell-min. e. p. p. frequency increased with increasing temperature. A logarithmic relation was observed with a mean Q 10 of 4.87 ± 0.37. In other experiments a Q 10 of 9.50 ± 0.63 was found for min. e. p. ps at innervated endplates. Lanthanum and manganese ions, black widow spider venom, ethanol, diamide, ouabain, theophylline and acid Ringer’s solution, all of which increased normal min. e. p. p. frequency, failed to raise the frequency of Schwann cell-min. e. p. ps. Lanthanum, ethanol, ouabain and low pH depressed the frequency, while black widow spider venom, diamide, theophyline and manganese were without significant effect. Adenosine, which depresses min. e. p. p. frequency at innervated endplates, had no effect on spontaneous miniature potentials at denervated endplates. Removal of Ca 2+ from the external medium reduced the frequency of Schwann min. e. p. ps; and a decrease was also seen in one experiment where the Ca 2+ concentration was raised from 1.8 to 10 mM. Diluting the Ringer’s solution raised Schwann cell-min. e. p. p. frequency transiently. This increase resulted from a reduction in osmolarity, not in the ionic strength, of the medium.

scholarly journals Particle Assembly and Ultrastructural Features Associated with Replication of the Lytic Archaeal Virus Sulfolobus Turreted Icosahedral Virus

2009 ◽  
Vol 83 (12) ◽  
pp. 5964-5970 ◽  
Author(s):  
Susan K. Brumfield ◽  
Alice C. Ortmann ◽  
Vincent Ruigrok ◽  
Peter Suci ◽  
Trevor Douglas ◽  
...  
Keyword(s):  
Time Course ◽  
Plaque Assay ◽  
Ultrastructural Changes ◽  
Progeny Virus ◽  
Particle Assembly ◽  
Virus Particles ◽  
Transmission Electron ◽  
Archaeal Viruses ◽  
Infected Cells ◽  
Sulfolobus Turreted Icosahedral Virus

ABSTRACT Little is known about the replication cycle of archaeal viruses. We have investigated the ultrastructural changes of Sulfolobus solfataricus P2 associated with infection by Sulfolobus turreted icosahedral virus (STIV). A time course of a near synchronous STIV infection was analyzed using both scanning and transmission electron microscopy. Assembly of STIV particles, including particles lacking DNA, was observed within cells, and fully assembled STIV particles were visible by 30 h postinfection (hpi). STIV was determined to be a lytic virus, causing cell disruption beginning at 30 hpi. Prior to cell lysis, virus infection resulted in the formation of pyramid-like projections from the cell surface. These projections, which have not been documented in any other host-virus system, appeared to be caused by the protrusion of the cell membrane beyond the bordering S-layer. These structures are thought to be sites at which progeny virus particles are released from infected cells. Based on these observations of lysis, a plaque assay was developed for STIV. From these studies we propose an overall assembly model for STIV.

scholarly journals Effect of Single Administration of Coffee on Pupil Size and Ocular Wavefront Aberration Measurements in Healthy Subjects

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Handan Bardak ◽  
Murat Gunay ◽  
Ugur Mumcu ◽  
Yavuz Bardak
Keyword(s):  
Pupil Size ◽  
Healthy Subjects ◽  
Spherical Aberration ◽  
Ultrastructural Changes ◽  
Wavefront Aberration ◽  
Single Administration ◽  
Coffee Intake ◽  
Coffee Drinking ◽  
Acute Changes ◽  
The Impact

No study has so far evaluated the impact of coffee drinking on ocular wavefront aberration (OWA) measurements. This study presents novel findings regarding the OWA of the eye following coffee intake. We aimed to evaluate the acute changes in pupil size and OWA of the eye after single administration of coffee. A total of 30 otherwise healthy participants were included in this prospective study. All subjects drank a cup of coffee containing 57 mg caffeine. Measurements of pupil size, total coma (TC), total trefoil (TF), total spherical aberration (TSA), and total higher order aberration (HOA) were performed before and at 5 minutes, at 30 minutes, and at 4 hours after coffee drinking using a wavefront aberrometer device (Irx3, Imagine Eyes, Orsay, France). The mean age of the study population was 20.30 ± 2.74 years. Pupil size did not show a significant change during the measurements (p>0.05). A significant increase was observed in TF and HOA measurements following coffee intake (p=0.029andp=0.009, resp.). Single administration of coffee results in significant increase in TF and total HOAs in healthy subjects without any effect on pupil diameter. Ultrastructural changes in the cornea following coffee intake might be of relevance to the alterations in ocular aberrations in healthy subjects.

Effect of reflex therapy on time course of ultrastructural changes in muscular branches of the brachial plexus

1987 ◽  
Vol 103 (1) ◽  
pp. 128-130
Author(s):  
A. M. Zagrebin ◽  
O. A. Khonny ◽  
V. M. Chuchkov ◽  
A. V. Isaev ◽  
T. B. Shirokova
Keyword(s):  
Brachial Plexus ◽  
Time Course ◽  
Ultrastructural Changes ◽  
Reflex Therapy

Doxorubicin concentration time course in the myocardium after single administration to the dog

1987 ◽  
Vol 20 (3) ◽  
pp. 267-269 ◽  
Author(s):  
Quadiri Timour ◽  
Patrice Nony ◽  
Jean Lang ◽  
Mohamed Lakhal ◽  
V�ronique Trillet ◽  
...  
Keyword(s):  
Time Course ◽  
Single Administration ◽  
Concentration Time ◽  
Doxorubicin Concentration

Cellular differentiation in Venturia inaequalis ascospores during germination and penetration of apple leaves

1987 ◽  
Vol 65 (12) ◽  
pp. 2549-2561 ◽  
Author(s):  
K. J. Smereka ◽  
W. E. Machardy ◽  
A. P. Kausch
Keyword(s):  
Time Course ◽  
Venturia Inaequalis ◽  
Ultrastructural Changes ◽  
Wall Material ◽  
Dense Matrix ◽  
Mechanical Pressure ◽  
Selective Staining ◽  
Infection Hypha ◽  
Dome Shape ◽  
Host Tissues

The penetration of the apple leaf cuticle by ascospores of Venturia inaequalis was studied by using ultrastructural and cytochemical techniques. A time-course developmental sequence is presented. Attachment of ascospores to the leaf surface appears to be by a mucilaginous substance and is followed by germination and penetration pore formation. The penetration pore is a circular opening adjacent to the leaf. It appears to form by degradation of the fungal wall and is bordered by a thickening of fungal wall material. Above the penetration pore, an infection sac forms from invagination and extension of the fungal plasmalemma. This structure has been reported only in V. inaequalis. Infection sac development initiates when the fungal plasmalemma appears to be forced into a dome shape. The dome flattens out and the membrane folds back upon itself, circumscribing the margin of the pore. The folded membrane becomes apposed, resulting in a circular belt of junctional structures which morphologically resemble molluscan septate junctions. As the infection sac enlarges, additional junctions form wherever the infection sac membrane abuts with the fungal plasmalemma. Selective staining with phosphotungstic acid revealed that the infection sac membrane becomes differentiated from the fungal plasmalemma. The infection sac enlarges and accumulates a dense matrix that appears to penetrate into the cuticle, causing ultrastructural changes in host tissues. The infection hypha, which is an extension of the infection sac membrane, breaches the cuticle without any apparent mechanical pressure.

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