Ultrastructural changes in motor endplates of the lumbrical muscles of rats induced by a microsomal Ca 2+ ATPase inhibitor, 2,5-di(tert-butyl)-1,4-hydroquinone

1997 ◽  
Vol 72 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Kunitoshi Mitsumori ◽  
Takayoshi Imazawa ◽  
Hiroshi Onodera ◽  
Michihito Takahashi ◽  
Satoshi Kitajima ◽  
...  
Keyword(s):  
Ultrastructural Changes ◽  
Atpase Inhibitor ◽  
Motor Endplates ◽  
Tert Butyl ◽  
Lumbrical Muscles

Evidence for two intracellular calcium pools in Dictyostelium: the cAMP-induced calcium influx is directed into a NBD-Cl- and 2,5-di-(tert-butyl)1,4-hydroquinone-sensitive pool

1993 ◽  
Vol 105 (4) ◽  
pp. 1131-1135 ◽  
Author(s):  
H. Flaadt ◽  
E. Jaworski ◽  
D. Malchow
Keyword(s):  
Calcium Influx ◽  
Fine Tuning ◽  
Cell Surface Receptor ◽  
Bafilomycin A1 ◽  
Atpase Inhibitor ◽  
Tert Butyl ◽  
Cellular Events ◽  
Surface Receptor ◽  
Oriented Movement ◽  
Gamma S

Signal transduction in Dictyostelium for oriented movement and differentiation involves a fine tuning of the cytosolic Ca2+ concentration. We have previously shown that cAMP binding to the cell surface receptor elicits two cellular events: (i) to enhance Ca2+ entry across the plasma membrane; (ii) to increase Ca2+ uptake into Ca(2+)-sequestering organelles. Here we used permeabilised cells to show that cAMP-induced Ca2+ uptake in these cells was sensitive to the Ca2+ transport ATPase blocker 2,5-di-(tert-butyl)-1,4-hydroquinone (BHQ) and the vacuolar H(+)-ATPase inhibitor NBD-Cl. By contrast, bafilomycin A1 and vanadate, inhibitors of Ca2+ uptake into acidosomes in Dictyostelium, did not reduce the cAMP-induced Ca2+ uptake of permeabilised cells. GTP gamma S served as a tool to measure Ins(1,4,5)P3- (InsP3)-sensitive Ca2+ release. Following NBD-Cl or BHQ treatment Ca2+ release was reversibly inhibited. We conclude that the cAMP-controlled Ca2+ influx is directed into a NBD-Cl and BHQ-sensitive compartment, which comprises the InsP3-releasable pool. The acidosomal Ca2+ store seems to provide for additional Ca2+ if required.

Response of human spermatozoa to an internal calcium ATPase inhibitor, 2,5-di(tert-butyl) hydroquinone

1997 ◽  
Vol 279 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Raquel L. Perry ◽  
Christopher L. R. Barratt ◽  
Michael A. Warren ◽  
Ian D. Cooke
Keyword(s):  
Calcium Atpase ◽  
Human Spermatozoa ◽  
Atpase Inhibitor ◽  
Tert Butyl ◽  
Internal Calcium

Vasoconstriction and bronchoconstriction induced by 2,5-Di(tert-butyl)1,4-benzohydroquinone, an endoplasmic reticular Ca2+-ATPase inhibitor in isolated and perfused rat lung

1992 ◽  
Vol 36 (1-2) ◽  
pp. 33-38 ◽  
Author(s):  
L. Atzori ◽  
G. Bannenberg ◽  
A. M. Corriga ◽  
Å Ryrfeldt ◽  
P. Moldeus
Keyword(s):  
Rat Lung ◽  
Atpase Inhibitor ◽  
Tert Butyl
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