Induced changes in phenolic acids and stilbenes in embryogenic cell cultures of Norway spruce by culture filtrate of Ascocalyx abietina

2008 ◽  
Vol 115 (2) ◽  
pp. 57-62 ◽  
Author(s):  
M. Cvikrová ◽  
J. Malá ◽  
M. Hrubcová ◽  
J. Eder ◽  
S. Foretová
Keyword(s):  
Norway Spruce ◽  
Phenolic Acids ◽  
Cell Cultures ◽  
Culture Filtrate ◽  
Embryogenic Cell ◽  
Induced Changes

scholarly journals Changes in phenolic acids and stilbenes induced in embryogenic cell cultures of Norway spruce by two fractions of Sirococcus strobilinus mycelian

2011 ◽  
Vol 57 (No. 1) ◽  
pp. 1-7 ◽  
Author(s):  
J. Malá ◽  
M. Hrubcová ◽  
P. Máchová ◽  
H. Cvrčková ◽  
O. Martincová ◽  
...  
Keyword(s):  
Cell Wall ◽  
Norway Spruce ◽  
Phenolic Acids ◽  
Cell Cultures ◽  
Total Content ◽  
Cell Suspension Cultures ◽  
Moderate Increase ◽  
Embryogenic Cell ◽  
Defence Responses ◽  
In Cells

We examined defence responses in embryogenic cell suspension cultures of Norway spruce (Picea abies [L.] Karst) elicited by intracellular protein and cell wall fractions (PF and WF, respectively) prepared from mycelia of the fungus Sirococcus strobilinus Preuss focusing on changes in (soluble and cell wall-bound) phenolic and stilbene concentrations. Treatment with both preparations induced an increase in the total contents of phenolic acids in Norway spruce cells and variations in the levels of stilbene glycosides. More rapid and intense induction of defence response was observed in cells after WF application. The contents of soluble phenolic acids (especially benzoic acid derivatives) and cell wall-bound phenolic acids (especially ferulic acid) started to increase (relative to controls) within 4 h after the addition of the WF preparation and remained high in elicited cells for 8–12 h. A moderate increase in phenolic acids in cells exposed to the PF preparation was observed within 8 h after application. However, after 24 h of WF treatment a decline of total phenolics was observed, while in PF elicited Norway spruce cells the phenolic content continued to increase. Significantly decreased concentrations of stilbene glycosides, isorhapontin, astringin and piceid, were determined in PF and WF treated Norway spruce cell cultures. The total content of stilbene glycosides decreased within 8 h after WF application to 68% of the amount determined in the control and within 12 h to 73% of the control in PF-treated cells. These results demonstrate that both PF and WF prepared from the Sirococcus strobilinus mycelium elicit changes in the metabolism of phenylpropanoids, which are involved in the defence responses of plants to pathogens.

Dark-induced changes in the content of phenolic acids in callus culture of alfalfa (Medicago sativa)

1990 ◽  
Vol 32 (3) ◽  
pp. 161-170 ◽  
Author(s):  
Milena Cvikrová ◽  
L. Meravý ◽  
Marie Hrubcová ◽  
J. Eder
Keyword(s):  
Medicago Sativa ◽  
Callus Culture ◽  
Phenolic Acids ◽  
Induced Changes

Polyamines and cytokinins in celery embryogenic cell cultures

1993 ◽  
Vol 12 (3) ◽  
pp. 245-254 ◽  
Author(s):  
M. Danin ◽  
S. J. Upfold ◽  
N. Levin ◽  
B. L. Nadel ◽  
A. Altman ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Embryogenic Cell

Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system

2013 ◽  
Vol 94 (6) ◽  
pp. 1231-1237 ◽  
Author(s):  
Daniela Borgognone ◽  
Mariateresa Cardarelli ◽  
Elvira Rea ◽  
Luigi Lucini ◽  
Giuseppe Colla
Keyword(s):  
Mineral Composition ◽  
Phenolic Acids ◽  
Floating System ◽  
Induced Changes

Acid-rain-induced changes in cuticles and Ca distribution in Scots pine and Norway spruce seedlings

1995 ◽  
Vol 25 (8) ◽  
pp. 1313-1325 ◽  
Author(s):  
Minna Turunen ◽  
Satu Huttunen ◽  
Jaana Back ◽  
Jukka Lamppu
Keyword(s):  
Acid Rain ◽  
Norway Spruce ◽  
Scots Pine ◽  
Crystal Formation ◽  
Water Control ◽  
Growing Seasons ◽  
Physicochemical Changes ◽  
Transmission Electron ◽  
Pine Seedlings ◽  
Induced Changes

Seedlings of Scots pine (Pinussylvestris L.) and Norway spruce (Piceaabies (L.) Karst.) were subjected to acid rain irrigation at pH 7, pH 4, and pH 3 three times a week during the growing seasons of 1986–1989 in a field experiment. Scanning and transmission electron microscopy, energy dispersive spectrometry, contact angle measurements, and chloroform extraction of waxes were used to detect physicochemical changes in the needle cuticles. The first detectable symptoms of acid rain were observed after 5 weeks of acid rain treatment at pH 3 and pH 4, which resulted in few CaSO4 crystallites on visibly undamaged pine and spruce needle surfaces. After 7 weeks of acid rain treatment there were CaSO4 crystallites scattered over the whole needle surface area and erosion of the epicuticular waxes could be observed occasionally. CaSO4 crystal formation later decreased, especially on the needles of seedlings treated at pH 3. Ca concentrations in the needles and roots of the seedlings and in the soil in the boxes were higher in the pH 3 treatments than elsewhere. The more abundant deposition of Ca oxalate crystallites on the inner walls of the epidermal and hypodermal cells of the spruce needles than on their outer walls was probably also connected with Ca leaching, caused by acid rain. Acid rain also delayed wax synthesis, as 2-month-old pine needles exposed to pH 3 and pH 4 had about 50% less wax than the water controls in early August. The needle surfaces of the southern provenances of spruce and pine seedlings were slightly less wettable after pH 4 treatment than after the control water treatment, because they probably benefited from N and S compounds in the irrigation water. The needle surfaces were more wettable in the pH 3 and water control seedlings than in the other treatments.

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