Polyamines and cytokinins in celery embryogenic cell cultures

1993 ◽  
Vol 12 (3) ◽  
pp. 245-254 ◽  
Author(s):  
M. Danin ◽  
S. J. Upfold ◽  
N. Levin ◽  
B. L. Nadel ◽  
A. Altman ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Embryogenic Cell

scholarly journals Changes in phenolic acids and stilbenes induced in embryogenic cell cultures of Norway spruce by two fractions of Sirococcus strobilinus mycelian

2011 ◽  
Vol 57 (No. 1) ◽  
pp. 1-7 ◽  
Author(s):  
J. Malá ◽  
M. Hrubcová ◽  
P. Máchová ◽  
H. Cvrčková ◽  
O. Martincová ◽  
...  
Keyword(s):  
Cell Wall ◽  
Norway Spruce ◽  
Phenolic Acids ◽  
Cell Cultures ◽  
Total Content ◽  
Cell Suspension Cultures ◽  
Moderate Increase ◽  
Embryogenic Cell ◽  
Defence Responses ◽  
In Cells

We examined defence responses in embryogenic cell suspension cultures of Norway spruce (Picea abies [L.] Karst) elicited by intracellular protein and cell wall fractions (PF and WF, respectively) prepared from mycelia of the fungus Sirococcus strobilinus Preuss focusing on changes in (soluble and cell wall-bound) phenolic and stilbene concentrations. Treatment with both preparations induced an increase in the total contents of phenolic acids in Norway spruce cells and variations in the levels of stilbene glycosides. More rapid and intense induction of defence response was observed in cells after WF application. The contents of soluble phenolic acids (especially benzoic acid derivatives) and cell wall-bound phenolic acids (especially ferulic acid) started to increase (relative to controls) within 4 h after the addition of the WF preparation and remained high in elicited cells for 8–12 h. A moderate increase in phenolic acids in cells exposed to the PF preparation was observed within 8 h after application. However, after 24 h of WF treatment a decline of total phenolics was observed, while in PF elicited Norway spruce cells the phenolic content continued to increase. Significantly decreased concentrations of stilbene glycosides, isorhapontin, astringin and piceid, were determined in PF and WF treated Norway spruce cell cultures. The total content of stilbene glycosides decreased within 8 h after WF application to 68% of the amount determined in the control and within 12 h to 73% of the control in PF-treated cells. These results demonstrate that both PF and WF prepared from the Sirococcus strobilinus mycelium elicit changes in the metabolism of phenylpropanoids, which are involved in the defence responses of plants to pathogens.

scholarly journals Somatic Embryogenesis in Arabidopsis thaliana Is Facilitated by Mutations in Genes Repressing Meristematic Cell Divisions

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 549-563
Author(s):  
Andreas P Mordhorst ◽  
Keete J Voerman ◽  
Marijke V Hartog ◽  
Ellen A Meijer ◽  
Jacques van Went ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cell Cultures ◽  
Egg Cell ◽  
Zygotic Embryos ◽  
Additive Effects ◽  
Additional Advantage ◽  
Embryogenic Cell ◽  
Immature Zygotic Embryos

Abstract Embryogenesis in plants can commence from cells other than the fertilized egg cell. Embryogenesis initiated from somatic cells in vitro is an attractive system for studying early embryonic stages when they are accessible to experimental manipulation. Somatic embryogenesis in Arabidopsis offers the additional advantage that many zygotic embryo mutants can be studied under in vitro conditions. Two systems are available. The first employs immature zygotic embryos as starting material, yielding continuously growing embryogenic cultures in liquid medium. This is possible in at least 11 ecotypes. A second, more efficient and reproducible system, employing the primordia timing mutant (pt allelic to hpt, cop2, and amp1), was established. A significant advantage of the pt mutant is that intact seeds, germinated in 2,4-dichlorophenoxyacetic acid (2,4-D) containing liquid medium, give rise to stable embryonic cell cultures, circumventing tedious hand dissection of immature zygotic embryos. pt zygotic embryos are first distinguishable from wild type at early heart stage by a broader embryonic shoot apical meristem (SAM). In culture, embryogenic clusters originate from the enlarged SAMs. pt somatic embryos had all characteristic embryo pattern elements seen in zygotic embryos, but with higher and more variable numbers of cells. Embryogenic cell cultures were also established from seedling, of other mutants with enlarged SAMs, such as clavata (clv). pt clv double mutants showed additive effects on SAM size and an even higher frequency of seedlings producing embryogenic cell lines. pt clv double mutant plants had very short fasciated inflorescence stems and additive effects on the number of rosette leaves. This suggests that the PT and CLV genes act in independent pathways that control SAM size. An increased population of noncommitted SAM cells may be responsible for facilitated establishment of somatic embryogenesis in Arabidopsis.

Morphogenetic effects of Brefeldin A on embryogenic cell cultures of Daucus carota L.

Planta ◽  
1997 ◽  
Vol 203 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Gloria Capitanio ◽  
Barbara Baldan ◽  
Francesco Filippini ◽  
Mario Terzi ◽  
Fiorella Lo Schiavo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Daucus Carota ◽  
Brefeldin A ◽  
Embryogenic Cell ◽  
Daucus Carota L ◽  
Morphogenetic Effects

Spontaneous and induced apoptosis in embryogenic cell cultures of carrot (Daucus carota L.) in different physiological states

2000 ◽  
Vol 79 (4) ◽  
pp. 294-298 ◽  
Author(s):  
Fiorella LoSchiavo ◽  
Barbara Baldan ◽  
Davide Compagnin ◽  
Rossella Ganz ◽  
Paola Mariani ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Daucus Carota ◽  
Embryogenic Cell ◽  
Daucus Carota L ◽  
Induced Apoptosis

Determining DNA strand breakage from embryogenic cell cultures of a conifer species using the single-cell gel electrophoresis assay

2011 ◽  
Vol 8 (2) ◽  
pp. 425-430 ◽  
Author(s):  
Pedro M. Costa ◽  
Ana Milhinhos ◽  
Marta Simões ◽  
Liliana Marum ◽  
Ana Maria Oliveira ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Cultures ◽  
Gel Electrophoresis ◽  
Conifer Species ◽  
Embryogenic Cell ◽  
Single Cell Gel Electrophoresis ◽  
Strand Breakage ◽  
Dna Strand ◽  
Dna Strand Breakage

Metabolism of Benzyladenine in Embryogenic Cell Cultures of Celery

1992 ◽  
Vol 140 (4) ◽  
pp. 466-469 ◽  
Author(s):  
J. Van Staden ◽  
S.J. Upfold ◽  
A. Altman ◽  
B.L. Nadel
Keyword(s):  
Cell Cultures ◽  
Embryogenic Cell
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