Dark-induced changes in the content of phenolic acids in callus culture of alfalfa (Medicago sativa)

1990 ◽  
Vol 32 (3) ◽  
pp. 161-170 ◽  
Author(s):  
Milena Cvikrová ◽  
L. Meravý ◽  
Marie Hrubcová ◽  
J. Eder
Keyword(s):  
Medicago Sativa ◽  
Callus Culture ◽  
Phenolic Acids ◽  
Induced Changes

Content of phenolic acids in callus culture of alfalfa (Medicago sativa): The effect of age and biochemical differentiation

1988 ◽  
Vol 30 (5) ◽  
pp. 321-326 ◽  
Author(s):  
Marie HrubcovÁ ◽  
Milena CvikrovÁ ◽  
F. Pospíšil ◽  
L. Meravý ◽  
J. Eder
Keyword(s):  
Medicago Sativa ◽  
Callus Culture ◽  
Phenolic Acids ◽  
Effect Of Age ◽  
Biochemical Differentiation

Insecticidal Active Rotenoids from Plant Parts and Callus Culture of Medicago sativa L. from a Semiarid Region of India (Rajasthan)

2020 ◽  
Vol 16 (6) ◽  
pp. 937-941
Author(s):  
Sharad Vats ◽  
Preeti Mehra
Keyword(s):  
Medicago Sativa ◽  
Callus Culture ◽  
Point Of View ◽  
Semiarid Region ◽  
Disease Vectors ◽  
Vector Borne Diseases ◽  
Plant Parts ◽  
Resistant Varieties ◽  
Medicago Sativa L

Background: Vector-borne diseases are quite prevalent globally and are one of the major causes of deaths due to infectious diseases. There is an availability of synthetic insecticides, however, their excessive and indiscriminate use have resulted in the emergence of resistant varieties of insects. Thus, a search for novel biopesticide has become inevitable. Methods: Rotenoids were isolated and identified from different parts of Medicago sativa L. This group of metabolites was also identified in the callus culture, and the rotenoid content was monitored during subculturing for a period of 10 months. Enhancement of the rotenoid content was evaluated by feeding precursors in a tissue culture medium. Results: Four rotenoids (elliptone, deguelin, rotenone and Dehydrorotenone) were identified, which were confirmed using spectral and chromatographic techniques. The maximum rotenoid content was found in the seeds (0.33±0.01%), followed by roots (0.31±0.01%) and minimum in the aerial parts (0.20±0.05%). A gradual decrease in the rotenoid content was observed with the ageing of subcultured tissue maintained for 10 months. The production of rotenoids was enhanced up to 2 folds in the callus culture using amino acids, Phenylalanine and Methionine as precursors as compared to the control. The LC50 value of the rotenoids was found to be 91 ppm and 162 ppm against disease vectors of malaria and Dracunculiasis, respectively. Conclusion: The study projects M. sativa as a novel source of biopesticide against the disease vectors of malaria and Dracunculiasis. The use of precursors to enhance the rotenoid content in vitro can be an effective venture from a commercial point of view.

Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system

2013 ◽  
Vol 94 (6) ◽  
pp. 1231-1237 ◽  
Author(s):  
Daniela Borgognone ◽  
Mariateresa Cardarelli ◽  
Elvira Rea ◽  
Luigi Lucini ◽  
Giuseppe Colla
Keyword(s):  
Mineral Composition ◽  
Phenolic Acids ◽  
Floating System ◽  
Induced Changes

Phenolic acids and peroxidase activity in alfalfa (Medicago sativa) embryogenic cultures after ethephon treatment

1994 ◽  
Vol 91 (2) ◽  
pp. 226-233 ◽  
Author(s):  
Milena Cvikrova ◽  
Marie Hrubcova ◽  
Martin Vagner ◽  
Ivana Machackova ◽  
Josef Eder
Keyword(s):  
Medicago Sativa ◽  
Phenolic Acids ◽  
Peroxidase Activity ◽  
Embryogenic Cultures ◽  
Ethephon Treatment

Changes in Source-Sink Relationship for Nitrogen During Regrowth of Lucerne (Medicago sativa L.) Following Removal of Shoots

1991 ◽  
Vol 18 (6) ◽  
pp. 593 ◽  
Author(s):  
TH Kim ◽  
A Ourry ◽  
J Boucaud ◽  
G Lemaire
Keyword(s):  
Amino Acid ◽  
Medicago Sativa ◽  
Hydroponic Culture ◽  
Intact Plants ◽  
Medicago Sativa L ◽  
Stems And Leaves ◽  
Almost All ◽  
Source Sink ◽  
Induced Changes ◽  
Nitrogen Remobilisation

An experiment with lucerne plants (Medicago sativa L.) previously labelled with 15N and grown in hydroponic culture, was undertaken to define sink and source behaviour of different organs in defoliated and intact plants. Changes in 15N contents of plants during 24 days of regrowth on an unlabelled medium after defoliation were used to estimate flows of exogenous and endogenous nitrogen. The 15N content of regrowing stems and leaves increased as a result of remobilisation mainly from lateral and tap roots which, therefore, acted as source organs. Nitrogen remobilisation reached a plateau after 10 days of regrowth and, until this time, nearly all N for shoot regrowth came from endogenous N in roots and crown. Between 25 and 35% of N reserves were translocated to regrowing stems, the remainder to regrowing leaves. Amino acid-N was the most readily available form of N while protein-N was the largest storage pool. Nitrogen uptake from the medium and accumulation in source organs (roots and crown) was significant only between days 6 and 14, and almost all was subsequently translocated to regrowing tissues. Defoliation induced changes in source-sink relationships for N. Whereas stems and tap roots were the main sink organs in intact plants, regrowing shoots exerted a stronger sink behaviour in defoliated plants.

scholarly journals Silicon-Induced Changes in Antifungal Phenolic Acids, Flavonoids, and Key Phenylpropanoid Pathway Genes during the Interaction between Miniature Roses and the Biotrophic Pathogen Podosphaera pannosa

2011 ◽  
Vol 157 (4) ◽  
pp. 2194-2205 ◽  
Author(s):  
Radhakrishna Shetty ◽  
Xavier Fretté ◽  
Birgit Jensen ◽  
Nandini Prasad Shetty ◽  
Jens Due Jensen ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Phenylpropanoid Pathway ◽  
Biotrophic Pathogen ◽  
Induced Changes

Phenolic acids and peroxidase activity in alfalfa (Medicago sativa) embryogenic cultures after ethephon treatment

1994 ◽  
Vol 91 (2) ◽  
pp. 226-233 ◽  
Author(s):  
Milena Cvikrova ◽  
Marie Hrubcova ◽  
Martin Vagner ◽  
Ivana Machackova ◽  
Josef Eder
Keyword(s):  
Medicago Sativa ◽  
Phenolic Acids ◽  
Peroxidase Activity ◽  
Embryogenic Cultures ◽  
Ethephon Treatment
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