Lipoprotein lipase and lipogenic enzyme activities in adipose tissue from rats fed different lipid sources

2001 ◽  
Vol 57 (3) ◽  
pp. 245-254 ◽  
Author(s):  
V. M. Rodríguez ◽  
M. T. Macarulla ◽  
M. Chávarri ◽  
D. Durán ◽  
M. P. Portillo
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Enzyme Activities ◽  
Lipogenic Enzyme

scholarly journals trans-10, cis-12 Conjugated linoleic acid inhibits lipoprotein lipase but increases the activity of lipogenic enzymes in adipose tissue from hamsters fed an atherogenic diet

2006 ◽  
Vol 95 (6) ◽  
pp. 1112-1119 ◽  
Author(s):  
Amaia Zabala ◽  
Itziar Churruca ◽  
Alfredo Fernández-Quintela ◽  
Víctor M. Rodríguez ◽  
M. Teresa Macarulla ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Linoleic Acid ◽  
Food Intake ◽  
Lipoprotein Lipase ◽  
Body Fat ◽  
Enzyme Activities ◽  
Serum Insulin ◽  
Mrna Levels ◽  
Lipogenic Enzyme ◽  
Lipogenic Enzymes

The aim of the present work was to investigate the effects of trans-10,cis-12conjugated linoleic acid (CLA) on the activity and expression of lipogenic enzymes and lipoprotein lipase (LPL), as well as on the expression of transcriptional factors controlling these enzymes, in adipose tissue from hamsters, and to evaluate the involvement of these changes in the body fat-reducing effect of this CLA isomer. Thirty male hamsters were divided into three groups and fed atherogenic diets supplemented with 0 (linoleic group), 5 or 10g trans-10,cis-12CLA/kg diet, for 6 weeks. Body and adipose tissue weights, food intake and serum insulin were measured. Total and heparin-releasable LPL and lipogenic enzyme activities (acetyl-CoA carboxylase (ACC); fatty acid synthase (FAS); glucose-6-phosphate dehydrogenase (G6PDH); and malic enzyme (ME)) were assessed. ACC, FAS, LPL, sterolregulatory element-binding proteins (SREBP-1a), SREBP-1c and PPARγ mRNA levels were alsodetermined by real-time PCR. CLA did not modify food intake, body weight and serum insulin level. CLA feeding reduced adipose tissue weight, LPL activity and expression, and increased lipogenic enzyme activities, despite a significant reduction in ACC and FAS mRNA levels. The expression of the three transcriptional factors analysed (SREBP-1a, SREBP-1c and PPARγ) wasalso reduced. These results appear to provide a framework for partially understanding the reduction in body fat induced by CLA. Inhibition of LPL activity seems to be an important mechanism underlying body fat reduction in hamsters. Further research is needed to better characterizethe effects of CLA on lipogenesis and the role of these effects in CLA action.

scholarly journals Adipose-tissue lipoprotein lipase activity and cellularity in the genetically obese Zucker rat (fa/fa) (Short Communication)

1973 ◽  
Vol 132 (3) ◽  
pp. 633-635 ◽  
Author(s):  
P. de Gasquet ◽  
E. Péquignot ◽  
D. Lemonnier ◽  
A. Alexiu
Keyword(s):  
Adipose Tissue ◽  
Surface Area ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Enzyme Activities ◽  
Short Communication ◽  
Zucker Rat ◽  
Lipoprotein Lipase Activity ◽  
Obese Zucker Rat ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase activity per adipocyte was increased in the genetically obese rat (fa/fa). However, there was no difference between obese and lean animals when the enzyme activities were related to adipocyte surface area. The possible implications of the findings are discussed.

Effects of isoenergetic barley grain and alfalfa pellet diets on in vitro lipogenesis and lipogenic enzyme activities in subcutaneous adipose tissue from sheep

1997 ◽  
Vol 77 (1) ◽  
pp. 173-175 ◽  
Author(s):  
E. K. Okine ◽  
P. F. Arthur
Keyword(s):  
Adipose Tissue ◽  
Enzyme Activities ◽  
Subcutaneous Adipose Tissue ◽  
Barley Grain ◽  
Lipogenic Enzyme ◽  
Acetyl Coa Carboxylase ◽  
Lipogenic Enzymes ◽  
Acetyl Coa ◽  
Subcutaneous Adipose

The effects of feeding isoenergetic barley grain or alfalfa pellets on in vitro lipogenesis, palmitate esterification, and lipogenic enzymes activities in biopsy samples of subcutaneous adipose tissue in 10 ewes were studied. Acetyl CoA carboxylase and FAS activities and rate of esterification were 52, 38, and 88%, respectively, higher (P < 0.05) in subcutaneous adipose of ewes fed the concentrate versus those fed the roughage diet. Key words: Isoenergetic, adipose tissue, acetyl CoA carboxylase

scholarly journals Free-Range and Low-Protein Concentrated Diets in Iberian Pigs: Effect on Plasma Insulin and Leptin Concentration, Lipogenic Enzyme Activity, and Fatty Acid Composition of Adipose Tissue

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1917
Author(s):  
Juan F. Tejeda ◽  
Alejandro Hernández-Matamoros ◽  
Elena González
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Enzyme Activities ◽  
Carcass Traits ◽  
Live Weight ◽  
Lipogenic Enzyme ◽  
Free Range ◽  
Low Protein ◽  
Fa Composition ◽  
Glycerol 3 Phosphate Dehydrogenase

The purpose of this study was to investigate the effect of diets with different protein contents on carcass traits, plasma hormone concentration, lipogenic enzyme activities, and fatty acid (FA) composition in the adipose tissue of Iberian pigs. Twenty-four castrated male Iberian pigs (eight per feeding diet) were fed under free-range conditions with acorns and grass (FR), and in confinement with concentrated diets with standard (SP) and low-protein contents (LP) from 116.0 to 174.2 kg live weight. Backfat thickness was not affected by diet. The plasma leptin concentration was higher (p < 0.001) in the FR group than in the LP and SP groups, while insulin concentration was higher in the SP group than in the LP and FR groups. The lipogenic enzyme activities of glucose-6-phosphate dehydrogenase, malic enzyme, and glycerol-3-phosphate dehydrogenase were lower in the FR group compared to the LP and SP pigs. The activities of these enzymes were adipose-tissue-specific. No differences were found in FA composition of adipose tissue between the SP and LP groups, while the FR pigs had lower proportions of saturated FA and higher proportions of monounsaturated and polyunsaturated FA than the SP and LP pigs. In conclusion, feeding low-protein diets in Iberian pigs does not seem to affect adipose carcass traits, strengthening previous findings that indicate that this is a good strategy to improve meat and dry-cured product quality.

Lipogenic enzyme activities and mRNAS in rat adipose tissue during weaning: Role of the diet

1990 ◽  
Vol 9 (1) ◽  
pp. VI
Author(s):  
C. Coupé ◽  
D. Perdereau ◽  
M.R. Narkewicz ◽  
P. Ferré ◽  
J. Girard
Keyword(s):  
Adipose Tissue ◽  
Enzyme Activities ◽  
Lipogenic Enzyme ◽  
Rat Adipose Tissue

Lipogenic enzyme activities and mRNA in rat adipose tissue at weaning

1990 ◽  
Vol 258 (1) ◽  
pp. E126-E133 ◽  
Author(s):  
C. Coupe ◽  
D. Perdereau ◽  
P. Ferre ◽  
Y. Hitier ◽  
M. Narkewicz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Enzyme Activities ◽  
Plasma Insulin ◽  
Regulation Of Gene Expression ◽  
Fatty Acid Synthetase ◽  
Lipogenic Enzyme ◽  
Lipogenic Enzymes ◽  
Suckling Rats ◽  
Force Feeding

The activities and mRNA concentrations of two lipogenic enzymes, fatty acid synthetase and acetyl-CoA carboxylase, and one enzyme involved in glyceroneogenesis, phosphoenolpyruvate carboxykinase (PEPCK), were measured in rat white adipose tissue during the suckling-weaning transition. Activities and mRNA concentrations of lipogenic enzymes were low in suckling rats, whereas activity and mRNA concentration of PEPCK were high. At weaning to a high-carbohydrate diet, the rapid increase in lipogenic enzymes mRNA (10- to 20-fold) and decrease in PEPCK mRNA (10-fold) were followed by parallel changes in enzyme activities. In contrast, weaning to a high-fat diet prevented these modifications. Force feeding suckling rats with carbohydrates induced a rise in blood glucose and plasma insulin concentrations. During these experiments, mRNA concentrations increased 10- to 20-fold for lipogenic enzymes and decreased 5-fold for PEPCK in less than 6 h, whereas all enzyme activities did not vary. This suggests a pretranslational regulation of gene expression. Force feeding suckling rats with a mixture of fat devoid of carbohydrate induced a slight increase in plasma insulin concentration and a fall in PEPCK mRNA but was not accompanied by a rise in lipogenic enzyme mRNAs. This suggested that insulin is a prime regulator of PEPCK gene expression, whereas glucose and insulin act synergistically in the regulation of lipogenic enzyme gene expression.

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