Chiral separation of β2-agonists by capillary electrophoresis using hydroxypropyl-α-cyclodextrin as a chiral selector

2001 ◽  
Vol 24 (4) ◽  
pp. 281-285 ◽  
Author(s):  
Kyeong Ho Kim ◽  
Hyun Ju Kim ◽  
Eun Young Jeun ◽  
Sang Hun Seo ◽  
Seon-Pyo Hong ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Separation ◽  
Chiral Selector ◽  
Β2 Agonists

scholarly journals Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2879
Author(s):  
Pavel Řezanka ◽  
Denisa Macková ◽  
Radek Jurok ◽  
Michal Himl ◽  
Martin Kuchař
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Separation ◽  
Simplex Method ◽  
Background Electrolyte ◽  
Detection Limits ◽  
Chiral Selector ◽  
Designer Drug ◽  
Optimal Composition ◽  
Linear Calibration

Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed. Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates. Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L−1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved. For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L−1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel’s fitting test, the linear calibration ranges were determined.

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