scholarly journals Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2879
Author(s):  
Pavel Řezanka ◽  
Denisa Macková ◽  
Radek Jurok ◽  
Michal Himl ◽  
Martin Kuchař
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Separation ◽  
Simplex Method ◽  
Background Electrolyte ◽  
Detection Limits ◽  
Chiral Selector ◽  
Designer Drug ◽  
Optimal Composition ◽  
Linear Calibration

Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed. Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates. Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L−1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved. For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L−1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel’s fitting test, the linear calibration ranges were determined.

scholarly journals Binding Constants Determination of Ofloxacin and Ornidazole Enantiomers with Sulfated β-Cyclodextrin as Single Ligand by Capillary Electrophoresis using Three Different Linear Plotting Methods

2021 ◽  
Vol 33 (7) ◽  
pp. 1663-1670
Author(s):  
Khaldun M. Al Azzam
Keyword(s):  
Capillary Electrophoresis ◽  
Background Electrolyte ◽  
Binding Constants ◽  
Chiral Selector ◽  
The Other ◽  
Binding Parameters ◽  
Guest Binding ◽  
Different Temperatures ◽  
Temperature Ranges

The aim of this research is to use capillary electrophoresis to establish/determine the binding constants for ofloxacin and ornidazole enantiomers with the negatively charged chiral selector sulfated-β-cyclodextrin (S-β-CD). Using electrophoretic mobility values of ofloxacin and ornidazole enantiomers at various concentrations of S-β-CD used in the context of background electrolyte (BGE), binding constants were calculated using three separate linearization plots, namely double-reciprocal, X-reciprocal and Y-reciprocal. The R-ofloxacin enantiomer-S-β-CD complex had the highest inclusion affinity of the ofloxacin and ornidazole enantiomers, which matched with previously reported estimation. Every enantiomer-S-β-CD complex’s binding constants, as well as thermodynamic binding parameters, were calculated at different temperatures. The host-guest binding constants using double reciprocal fit showed greater linearity (R2 > 0.99) at all temperature ranges measured (15-30 ºC) as compared to the other two fit approaches. The thermodynamic complexation parameters were found to be dependent on the temperature of the enantiomers, as seen by the linear van’t Hoff (15-30 ºC) plot.

Determination of enantiomeric excess for 2,3-dihydroxy-3-phenylpropionate compounds by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as chiral selector

Chirality ◽  
2007 ◽  
Vol 19 (5) ◽  
pp. 380-385 ◽  
Author(s):  
Yan Zhao ◽  
Xing-Bin Yang ◽  
Qiao-Feng Wang ◽  
Peng-Juan Nan ◽  
Ying Jin ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Enantiomeric Excess ◽  
Chiral Selector

scholarly journals Optimisation of analytical conditions for simultaneous determination of phenol and chlorophenols by capillary electrophoresis with indirect laser-induced fluorescence detection

2021 ◽  
Vol 63 (11) ◽  
pp. 51-55
Author(s):  
Manh Huy Nguyen ◽  
◽  
Hong Anh Duong ◽  
Hung Viet Pham ◽  
◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Phenolic Compounds ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Background Electrolyte ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Electrophoresis Method ◽  
Wastewater Samples

This work presented a capillary electrophoresis method with indirect laser-induced fluorescence detection for the simultaneous determination of phenol and four chlorophenol derivatives. The separation was obtained within 20 minutes with a background electrolyte composed of 5 mM borax and 1 mM fluorescein (pH=9.75), 17 kV of applied voltage, and 120 s of hydrostatic injection. At the optimal conditions, the limit of detections for phenolic compounds was in the range of 0.08-0.23 mg/l, and the RSD data of repeatability and reproducibility were less than 8.0% for both migration times and peak areas. This developed method was applied to analyse concentrations of phenolic compounds in surface water and wastewater samples, with the recoveries ranging from 59.4 to 102.5%.

Separation and determination of ephedrine enantiomers by capillary electrophoresis usingl-leucine as chiral selector

2002 ◽  
Vol 56 (9-10) ◽  
pp. 637-639 ◽  
Author(s):  
Nengsheng Ye ◽  
Xuexin Gu ◽  
Hong Zou ◽  
Ruohua Zhu
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Selector
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