Studies on soybean seed proteins by means of disc electrophoresis

1969 ◽  
Vol 11 (2) ◽  
pp. 183-189
Author(s):  
V. I. Safonov ◽  
M. P. Safonova ◽  
N. A. Narbut
Keyword(s):  
Soybean Seed ◽  
Seed Proteins ◽  
Disc Electrophoresis

GENETICS OF A PRIMARY TRISOMIC SERIES IN BARLEY: IDENTIFICATION BY PROTEIN ELECTROPHORESIS

1970 ◽  
Vol 12 (3) ◽  
pp. 490-495 ◽  
Author(s):  
R. G. McDaniel ◽  
R. T. Ramage
Keyword(s):  
Extra Chromosome ◽  
Seed Proteins ◽  
Disc Electrophoresis ◽  
Protein Electrophoresis ◽  
Primary Trisomics

Disc electrophoresis of seed proteins identified the extra chromosome involved in primary trisomics of barley. Each of the seven primary trisomics could be distinguished from diploids and from each other. Electrophoresis provided a precise biochemical criterion for identification of trisomics.

Electrophoretic Analyses of Soybean Seed Proteins

1992 ◽  
pp. 143-158 ◽  
Author(s):  
B. R. Hedges ◽  
R. G. Palmer ◽  
L. A. Amberger
Keyword(s):  
Soybean Seed ◽  
Seed Proteins

scholarly journals Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins

2005 ◽  
Vol 342 (2) ◽  
pp. 214-220 ◽  
Author(s):  
Savithiry Natarajan ◽  
Chenping Xu ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Proteomic Analysis ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Protein Solubilization

Mass spectrometry analysis of soybean seed proteins: optimization of gel-free quantitative workflow

2010 ◽  
Vol 2 (10) ◽  
pp. 1577 ◽  
Author(s):  
Dong-Gi Lee ◽  
Norma L. Houston ◽  
Severin E. Stevenson ◽  
Gregory S. Ladics ◽  
Scott McClain ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis

scholarly journals Polyacrylamide gel electrophoresis of soybean seed proteins

2015 ◽  
Vol 47 (4) ◽  
pp. 371-378 ◽  
Author(s):  
W. Lassocińsk ◽  
J. S. Knypl
Keyword(s):  
Glycine Max ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Soluble Proteins ◽  
Polyacrylamide Gel ◽  
Major Protein ◽  
Molecular Weights ◽  
Minor Protein

Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were detected under dissociating conditions. Molecular weights of three major protein fractions in PAGE SDS were determined for around 18 500, 36 000 and 80 000 daltons.

A comparison of the soluble seed proteins of certain Acer species

1971 ◽  
Vol 49 (11) ◽  
pp. 1951-1957 ◽  
Author(s):  
Theodore T. Ziegenfus ◽  
Roy B. Clarkson
Keyword(s):  
North America ◽  
Seed Proteins ◽  
Double Diffusion ◽  
Disc Electrophoresis ◽  
High Protein ◽  
The Other ◽  
Morphological Data ◽  
Eastern North America ◽  
Low Protein

Seed proteins of seven taxa of Acer native to eastern North America were obtained by extraction in 2.5% saline buffer, pH 7.0. These were investigated using the techniques of polyacrylamide disc electrophoresis, immunoelectrophoresis, and double diffusion. Groupings indicated by our protein data were compared with groupings by other researchers based on morphological data. The members of the section Saccharina, A. saccharum subsp. saccharum, A. s. subsp. nigrum and A. s. subsp. floridanum, were found to have close protein similarity. A. negundo, thought by several workers to be the most distinct of the maples, was found to have relatively high protein similarity with the members of the section Saccharina. A. rubrum and A. spicatum were shown to have the least protein similarity to the other taxa investigated. There is low protein similarity between these two taxa, however, indicating they are divergent. The traditional placement of A. rubrum and A. saccharinum in the same section was supported by the relatively high protein similarity of these taxa. Data obtained by serological techniques and polyacrylamide disc electrophoresis were found to be complementary. However, polyacrylamide disc electrophoresis was more sensitive in detecting the protein differences among the taxa investigated.

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