Heterogeneity of soybean seed proteins: one-dimensional electrophoretic profiles of six different solubility fractions

1981 ◽  
Vol 29 (3) ◽  
pp. 497-501 ◽  
Author(s):  
Branda Hu ◽  
Asim Esen
Keyword(s):  
Soybean Seed ◽  
Seed Proteins ◽  
One Dimensional

Electrophoretic Analyses of Soybean Seed Proteins

1992 ◽  
pp. 143-158 ◽  
Author(s):  
B. R. Hedges ◽  
R. G. Palmer ◽  
L. A. Amberger
Keyword(s):  
Soybean Seed ◽  
Seed Proteins

scholarly journals Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins

2005 ◽  
Vol 342 (2) ◽  
pp. 214-220 ◽  
Author(s):  
Savithiry Natarajan ◽  
Chenping Xu ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Proteomic Analysis ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Protein Solubilization

Mass spectrometry analysis of soybean seed proteins: optimization of gel-free quantitative workflow

2010 ◽  
Vol 2 (10) ◽  
pp. 1577 ◽  
Author(s):  
Dong-Gi Lee ◽  
Norma L. Houston ◽  
Severin E. Stevenson ◽  
Gregory S. Ladics ◽  
Scott McClain ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis

scholarly journals Polyacrylamide gel electrophoresis of soybean seed proteins

2015 ◽  
Vol 47 (4) ◽  
pp. 371-378 ◽  
Author(s):  
W. Lassocińsk ◽  
J. S. Knypl
Keyword(s):  
Glycine Max ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Soluble Proteins ◽  
Polyacrylamide Gel ◽  
Major Protein ◽  
Molecular Weights ◽  
Minor Protein

Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were detected under dissociating conditions. Molecular weights of three major protein fractions in PAGE SDS were determined for around 18 500, 36 000 and 80 000 daltons.

scholarly journals Isolation of Polyribosomes and Messenger RNA Active in in Vitro Synthesis of Soybean Seed Proteins

1978 ◽  
Vol 61 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Roger N. Beachy ◽  
John F. Thompson ◽  
James T. Madison
Keyword(s):  
Messenger Rna ◽  
Soybean Seed ◽  
Seed Proteins ◽  
In Vitro Synthesis

scholarly journals Trypsin Inhibitor Assessment with Biochemical and Molecular Markers in a Soybean Germplasm Collection and Hybrid Populations for Seed Quality Improvement

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Kulpash Bulatova ◽  
Shynar Mazkirat ◽  
Svetlana Didorenko ◽  
Dilyara Babissekova ◽  
Mukhtar Kudaibergenov ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Seed Quality ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Germplasm Collection ◽  
F1 Hybrid ◽  
Soybean Germplasm ◽  
Hybrid Plants ◽  
Very High ◽  
Two Hybrid

A soybean germplasm collection was studied for the identification of accessions with low trypsin inhibitor content in seeds. Twenty-nine accessions, parental plants, and two hybrid populations were selected and analyzed using genetic markers for alleles of the Ti3 locus, encoding Kunitz trypsin inhibitor (KTI). Most of the accessions had high or very high KTI (49.22–73.53 Trypsin units inhibited (TUI/mg seeds), while the two local Kazakh cultivars, Lastochka and Ivushka, were found to have a moderately high content of KTI, at 54.16–54.87 TUI/mg. In contrast, two soybean cultivars from Italy, Hilario and Ascasubi, showed the lowest levels of trypsin units inhibited, at 25.47–27.87 TUI/mg. Electrophoresis of seed proteins in a non-denaturing system showed a simple discrimination pattern and very clear presence/absence of bands corresponding to KTI. The SSR marker Satt228 was the most effective diagnostic marker among the three examined, and it confirmed the presence of the homozygous null-allele ti3/ti3 in cultivars Ascasubi and Hilario, which were used for hybridization with the local cv. Lastochka. Heterozygote F1 hybrid plants and homozygous ti3/ti3 lines in F2 segregating populations were successfully identified using Satt228. Finally, through marker-assisted selection with Satt228, prospective homozygous ti3/ti3 lines were produced for further application in the breeding program aimed at improving soybean seed quality in Kazakhstan.

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