A method of storage of leaf samples for chlorophyll analysis

1959 ◽  
Vol 1 (4) ◽  
pp. 287-294 ◽  
Author(s):  
Zdeněk Šesták
Keyword(s):  
Chlorophyll Analysis

Effects of cadmium and zinc on ozone-induced phytotoxicity in cress and lettuce

1974 ◽  
Vol 52 (3) ◽  
pp. 645-649 ◽  
Author(s):  
M. Czuba ◽  
D. P. Ormrod
Keyword(s):  
Foliar Application ◽  
Leaf Damage ◽  
Lepidium Sativum ◽  
Soil Application ◽  
Pigment Degradation ◽  
Grand Rapids ◽  
Lepidium Sativum L ◽  
Chlorophyll Analysis

Cadmium or zinc solutions were applied to the foliage or roots of lettuce (Lactuca saliva L. cv. Grand Rapids) and cress (Lepidium sativum L. cv. Fine Curled) at concentrations of 100 parts per million (ppm) every 4th day for several weeks. Four weeks after sowing, plants were fumigated once with 30-35 parts per hundred million (pphm) ozone, for 6 h. Cress plants that had received soil application of cadmium showed markedly increased ozone-induced phytotoxicity in terms of visible leaf damage and pigment degradation; in lettuce, only pigment degradation was evident based on chlorophyll analysis. Soil application of zinc showed less ozone-induced phytotoxicity on cress and no pigment degradation in lettuce. Compared with soil application, foliar application of zinc or cadmium produced less effects on ozone phytotoxicity.

scholarly journals Analisis Kandungan Klorofil Pada Beberapa Posisi Anak Daun Aren (Arenga pinnata) dengan Spektrofotometer UV-Vis

Jurnal MIPA ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 49
Author(s):  
Lydia Priskila Kamagi ◽  
Julius Pontoh ◽  
Lidya Irma Momuat
Keyword(s):  
Content Analysis ◽  
Solvent Extraction ◽  
Chlorophyll Content ◽  
Sugar Cane ◽  
Extraction Method ◽  
Human Life ◽  
Chlorophyll B ◽  
Solvent Extraction Method ◽  
The Right ◽  
Chlorophyll Analysis

Tanaman aren merupakan salah satu tanaman penghasil gula bagi kehidupan manusia.  Tanaman ini merupakan tanaman penghasil gula tertinggi daripada tanaman tebu dan tanaman bit.  Kemampuan tanaman aren dalam memproduksi gula sangat berhubungan dengan kandungan klorofil pada bagian daun. Penelitian ini dilakukan untuk mengembangkan metode analisis klorofil pada daun dan menentukan posisi anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a yang terdapat pada daun aren bervariasi, yaitu berkisar  22 - 39 µg/mL ; 1,5 - 2,3 mg/g ; 633 - 1.000 µmol /m 2 sedangkan untuk klorofil b yang terdapat pada daun aren bervariasi sekitar 6 - 10 µg/mL ; 0,4 - 0,7mg/g ;170 - 293 µmol/m 2.Posisi anak daun pada daun memiliki kandunngan klorofil yang lebih tinggi pada bagian atas, sedangkan yang terendah pada bagian bawah. Pada posisi kiri pada daun aren,kandungan klorofilnya lebih tinggi dibandingkan pada posisi kanan,sedangkan posisi pangkal, tengah, dan ujung pada anak daun hampir sama kandungan klorofilnya.Sugar palm is one of the sugar-producing plants for human life. This plant can produce sugar higher than a sugar-producing plant with sugar cane and beet plant. The ability of sugar palm in producing sugar is related to chlorophyll content in the leaf. This research was conducted to develop the method of chlorophyll analysis in the leaf and determine the position of leaflet that have the highest chlorophyll content. Analysis of chlorophyll content was performed by solvent extraction method that absorbance was read on UV-Vis spectrophotometer. The content of chlorophyll present in palm leaves varies, ranging from 22 - 39 μg /mL; 1.5- 2.3 mg/g or 633- 1,000 μmol/m2 whereas for chlorophyll b present in sugar palm varies from about 6 - 10 μg/mL; 0.4 -0.7 mg/g or 170 - 293 μmol/m2. The leaflet position on the leaves has a higher chlorophyll content at the top, while the lowest is at the bottom. In the left position on the palm leaves, chlorophyll content is higher than the right position, while the position of base,middle,and end of the leaflet leaves almost the same content.

scholarly journals Analysis of Chlorophyll Concentration in Potato Crop by Coupling Continuous Wavelet Transform and Spectral Variable Optimization

2020 ◽  
Vol 12 (17) ◽  
pp. 2826
Author(s):  
Ning Liu ◽  
Zizheng Xing ◽  
Ruomei Zhao ◽  
Lang Qiao ◽  
Minzan Li ◽  
...  
Keyword(s):  
Wavelet Transform ◽  
Continuous Wavelet Transform ◽  
Chlorophyll Concentration ◽  
Partial Least Square ◽  
Coefficient Of Determination ◽  
Good Prediction ◽  
Continuous Wavelet ◽  
Potato Crops ◽  
Chlorophyll Analysis

The analysis of chlorophyll concentration based on spectroscopy has great importance for monitoring the growth state and guiding the precision nitrogen management of potato crops in the field. A suitable data processing and modeling method could improve the stability and accuracy of chlorophyll analysis. To develop such a method, we collected the modelling data by conducting field experiments at the tillering, tuber-formation, tuber-bulking, and tuber-maturity stages in 2018. A chlorophyll analysis model was established using the partial least-square (PLS) algorithm based on original reflectance, standard normal variate reflectance, and wavelet features (WFs) under different decomposition scales (21–210, Scales 1–10), which were optimized by the competitive adaptive reweighted sampling (CARS) algorithm. The performances of various models were compared. The WFs under Scale 3 had the strongest correlation with chlorophyll concentration with a correlation coefficient of −0.82. In the model calibration process, the optimal model was the Scale3-CARS-PLS, which was established based on the sensitive WFs under Scale 3 selected by CARS, with the largest coefficient of determination of calibration set (Rc2) of 0.93 and the smallest Rc2−Rcv2 value of 0.14. In the model validation process, the Scale3-CARS-PLS model had the largest coefficient of determination of validation set (Rv2) of 0.85 and the smallest root–mean–square error of cross-validation (RMSEV) value of 2.77 mg/L, demonstrating good prediction capability of chlorophyll concentration. Finally, the analysis performance of the Scale3-CARS-PLS model was measured using the testing data collected in 2020; the R2 and RMSE values were 0.69 and 3.36 mg/L, showing excellent applicability. Therefore, the Scale3-CARS-PLS model could be used to analyze chlorophyll concentration. This study indicated the best decomposition scale of continuous wavelet transform and provided an important support method for chlorophyll analysis in the potato crops.

Modified Dimethyl Sulfoxide (DMSO) Extraction for Chlorophyll Analysis of Phytoplankton

1980 ◽  
Vol 37 (4) ◽  
pp. 729-733 ◽  
Author(s):  
B. K. Burnison
Keyword(s):  
Glass Fiber ◽  
Dimethyl Sulfoxide ◽  
Chlorophyll A ◽  
Extraction Procedure ◽  
Acetone Extraction ◽  
The Difference ◽  
Natural Phytoplankton ◽  
Chlorophyll Analysis

This new extraction procedure eliminates the need for grinding glass fiber filters by heating the filter in DMSO (4 mL) for 10 min at 65 °C and subsequently filtering or centrifuging the sample after dilution with 90% acetone (6 mL). Scenedesmus and Selenastrum species gave about 20% higher concentrations of chlorophyll a using this method as compared to the standard 90% acetone procedure or grinding in DMSO only. The difference between heated DMSO and 90% acetone was reduced when natural phytoplankton were analyzed.Key words: chlorophyll, Chlorophyta, dimethyl sulfoxide, acetone, extraction

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