scholarly journals In vitro inhibition of lipid peroxidation in fish by turmeric (Curcuma longa)

2006 ◽  
Vol 21 (2) ◽  
pp. 138-141 ◽  
Author(s):  
Hilda Priya D'Souza ◽  
H. Ramachandra Prabhu
Keyword(s):  
Lipid Peroxidation ◽  
Curcuma Longa ◽  
In Vitro Inhibition

In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Ganiyu Oboh ◽  
Adeniyi Abiodun Adebayo ◽  
Ayokunle Olubode Ademosun ◽  
Aline August Boligon
Keyword(s):  
Lipid Peroxidation ◽  
Dependent Manner ◽  
Inhibitory Property ◽  
In Vitro Inhibition ◽  
Anogeissus Leiocarpus ◽  
Dose Dependent ◽  
Phosphodiesterase 5

AbstractBackground:andMethods:The effects of the extracts on important enzymes (PDE-5 and arginase) linked with ED and pro-oxidants (FeResults:The results showed that both extracts inhibited PDE-5 and arginase activities in a dose-dependent manner. Inhibitory property ofConclusions:The ability of the extracts to inhibit PDE-5, arginase and pro-oxidant induced lipid peroxidation, and chelate metal might suggest their folkloric use for the management of ED.

scholarly journals Inhibition of Lipid Peroxidation of Kiwicha (Amaranthus caudatus) Hydrolyzed Protein Using Zebrafish Larvae and Embryos

Plants ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 69 ◽  
Author(s):  
Rubén Vilcacundo ◽  
Daniel Barrio ◽  
Lucrecia Piñuel ◽  
Patricia Boeri ◽  
Andrea Tombari ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Zebrafish Embryo ◽  
Oxygen Species ◽  
Zebrafish Larvae ◽  
Intestinal Phase ◽  
Hydrolyzed Protein ◽  
A Value ◽  
Gastrointestinal Conditions ◽  
In Vitro Inhibition

Amaranth protein concentrate (APC) was hydrolyzed under in vitro gastrointestinal conditions. APC proteins were partially degraded by pepsin at pHs 1.2, 2.0, and 3.2. During the intestinal phase (pepsin/pancreatin enzymes at pH 7.0), no polypeptide bands were observed in the gel, suggesting the susceptibility of amaranth proteins to the action of digestive enzymes. The potent in vitro inhibition of lipid peroxidation, shown by the gastric and intestinal digests, was confirmed in the zebrafish larvae, with a 72.86% reduction in oxidation of lipids in the presence of the gastric hydrolysate at pH 2.0, compared to a 95.72% reduction in the presence of the gastrointestinal digest. APC digests were capable of reducing reactive oxygen species (ROS) production in the zebrafish embryo model with a value of fluorescence of 52.5% for the gastric hydrolysate, and 48.4% for the intestinal hydrolysate.

scholarly journals Antioxidative Properties and Effect of Quercetin and Its Glycosylated Form (Rutin) on Acetylcholinesterase and Butyrylcholinesterase Activities

2016 ◽  
Vol 21 (4) ◽  
pp. NP11-NP17 ◽  
Author(s):  
Ayokunle O. Ademosun ◽  
Ganiyu Oboh ◽  
Fatai Bello ◽  
Peluola O. Ayeni
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Radical Scavenging ◽  
Antioxidative Properties ◽  
Chelating Ability ◽  
In Vitro Inhibition

This study sought to investigate the anticholinesterase and antioxidative properties of quercetin and its glycosylated conjugate, rutin. The in vitro inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities, inhibition of Fe2+-induced lipid peroxidation in rat’s brain homogenates, radicals scavenging, and Fe2+-chelating abilities of the flavonoids were investigated in vitro with concentrations of the samples ranging from 0.06 to 0.6 mM. Quercetin had significantly higher AChE and BChE inhibitory abilities than rutin. Quercetin also had stronger inhibition of Fe2+-induced lipid peroxidation in rat’s brain homogenates. Similarly, quercetin had higher radical scavenging abilities than rutin. Quercetin also had stronger Fe2+-chelating ability than rutin. The inhibition of cholinesterases and antioxidative properties are possible mechanisms by which the flavonoids can be used in the management of oxidative stress–induced neurodegeneration.

scholarly journals In-vitro Antioxidant and Anti-lipid Peroxidation Activity of Ethanolic Extracts of Bougainvillea shubhra, Bougainvillea peruviana and Bougainvillea bhuttiana Golden Glow: A Comparative Study

2015 ◽  
Vol 15 (1) ◽  
pp. 43 ◽  
Author(s):  
Mosmi Medpilwar ◽  
Darshil Maru ◽  
Meenakshi Upadhyay ◽  
Neha Lavania ◽  
Madhavi Vernekar ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Frap Assay ◽  
Ethanolic Extracts ◽  
In Vitro Inhibition ◽  
Reducing Power Assay

The aim of our present study was to evaluate the antioxidant and anti-lipid peroxidation activity of ethanolic extracts of <em>Bougainvillea species viz B. shubhra, B. peruviana and B. bhuttiana</em> golden glow. Phytochemical constituents viz. total phenolics, flavonoids and tannins were assayed using standard protocol. The antioxidant activity was studied by DPPH assay, FRAP assay, reducing power assay and in-vitro inhibition of lipid peroxidation. Of the three species, <em>B. bhuttiana</em> golden glow showed highest amount of phenols (6.78±0.001), flavonoids (27.7±0.012) and tannin (11.08±0.008) contents. It also showed highest antioxidant activity of 15.15± 0.008 and 8.08± 0.018 as determined by FRAP assay and reducing power assay respectively. The percent DPPH radical scavenging activity was 82.72% and in-vitro inhibition of lipid peroxidation in mitochondrial membrane was also found to be highest in <em>B. bhuttiana</em> golden glow.

Effekte von Substanz P und Neurotensin auf die Kontraktilität des Ileums der Maus in vitro: Inhibition durch STW 5

2007 ◽  
Vol 45 (08) ◽  
Author(s):  
D Hagelauer ◽  
O Kelber ◽  
D Weiser ◽  
S Laufer ◽  
H Heinle
Keyword(s):  
Substanz P ◽  
In Vitro Inhibition

Chemosensitizing Activity of Histone Deacetylases Inhibitory Cyclic Hydroxamic Acids for Combination Chemotherapy of Lymphatic Leukemia

2018 ◽  
Vol 18 (4) ◽  
pp. 365-371 ◽  
Author(s):  
Denis V. Mishchenko ◽  
Margarita E. Neganova ◽  
Elena N. Klimanova ◽  
Tatyana E. Sashenkova ◽  
Sergey G. Klochkov ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Acute Toxicity ◽  
Histone Deacetylases ◽  
Hydroxamic Acids ◽  
Water Soluble ◽  
Male Rat ◽  
Hybrid Male ◽  
Cyclic Hydroxamic Acids

Background: Anti-tumor effect of hydroxamic acid derivatives is largely connected with its properties as efficient inhibitors of histone deacetylases, and other metalloenzymes involved in carcinogenesis. Objective: The work was aimed to (i) determine the anti-tumor and chemosensitizing activity of the novel racemic spirocyclic hydroxamic acids using experimental drug sensitive leukemia P388 of mice, and (ii) determine the structure-activity relationships as metal chelating and HDAC inhibitory agents. Method: Outbreed male rat of 200-220 g weights were used in biochemical experiments. In vivo experiments were performed using the BDF1 hybrid male mice of 22-24 g weight. Lipid peroxidation, Fe (II) -chelating activity, HDAC fluorescent activity, anti-tumor and anti-metastatic activity, acute toxicity techniques were used in this study. Results: Chemosensitizing properties of water soluble cyclic hydroxamic acids (CHA) are evaluated using in vitro activities and in vivo methods and found significant results. These compounds possess iron (II) chelating properties, and slightly inhibit lipid peroxidation. CHA prepared from triacetonamine (1a-e) are more effective Fe (II) ions cheaters, as compared to CHA prepared from 1- methylpiperidone (2a-e). The histone deacetylase (HDAC) inhibitory activity, lipophilicity and acute toxicity were influenced by the length amino acids (size) (Glycine < Alanine < Valine < Leucine < Phenylalanine). All compounds bearing spiro-N-methylpiperidine ring (2a-e) are non-toxic up to 1250 mg/kg dose, while compounds bearing spiro-tetramethylpiperidine ring (1a-e) exhibit moderate toxicity which increases with increasing lipophility, but not excite at 400 mg/kg. Conclusion: It was shown that the use of combination of non-toxic doses of cisplatin (cPt) or cyclophosphamide with CHA in most cases result in the appearance of a considerable anti-tumor effect of cytostatics. The highest chemosensitizing activity with respect to leukemia Р388 is demonstrated by the CHA derivatives of Valine 1c or 2c.

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