scholarly journals Inhibition of Lipid Peroxidation of Kiwicha (Amaranthus caudatus) Hydrolyzed Protein Using Zebrafish Larvae and Embryos

Plants ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 69 ◽  
Author(s):  
Rubén Vilcacundo ◽  
Daniel Barrio ◽  
Lucrecia Piñuel ◽  
Patricia Boeri ◽  
Andrea Tombari ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Zebrafish Embryo ◽  
Oxygen Species ◽  
Zebrafish Larvae ◽  
Intestinal Phase ◽  
Hydrolyzed Protein ◽  
A Value ◽  
Gastrointestinal Conditions ◽  
In Vitro Inhibition

Amaranth protein concentrate (APC) was hydrolyzed under in vitro gastrointestinal conditions. APC proteins were partially degraded by pepsin at pHs 1.2, 2.0, and 3.2. During the intestinal phase (pepsin/pancreatin enzymes at pH 7.0), no polypeptide bands were observed in the gel, suggesting the susceptibility of amaranth proteins to the action of digestive enzymes. The potent in vitro inhibition of lipid peroxidation, shown by the gastric and intestinal digests, was confirmed in the zebrafish larvae, with a 72.86% reduction in oxidation of lipids in the presence of the gastric hydrolysate at pH 2.0, compared to a 95.72% reduction in the presence of the gastrointestinal digest. APC digests were capable of reducing reactive oxygen species (ROS) production in the zebrafish embryo model with a value of fluorescence of 52.5% for the gastric hydrolysate, and 48.4% for the intestinal hydrolysate.

In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Ganiyu Oboh ◽  
Adeniyi Abiodun Adebayo ◽  
Ayokunle Olubode Ademosun ◽  
Aline August Boligon
Keyword(s):  
Lipid Peroxidation ◽  
Dependent Manner ◽  
Inhibitory Property ◽  
In Vitro Inhibition ◽  
Anogeissus Leiocarpus ◽  
Dose Dependent ◽  
Phosphodiesterase 5

AbstractBackground:andMethods:The effects of the extracts on important enzymes (PDE-5 and arginase) linked with ED and pro-oxidants (FeResults:The results showed that both extracts inhibited PDE-5 and arginase activities in a dose-dependent manner. Inhibitory property ofConclusions:The ability of the extracts to inhibit PDE-5, arginase and pro-oxidant induced lipid peroxidation, and chelate metal might suggest their folkloric use for the management of ED.

scholarly journals In Vitro Inhibition of Streptococcus mutans Biofilm Formation on Hydroxyapatite by Subinhibitory Concentrations of Anthraquinones

2007 ◽  
Vol 51 (4) ◽  
pp. 1541-1544 ◽  
Author(s):  
Tom Coenye ◽  
Kris Honraet ◽  
Petra Rigole ◽  
Pol Nadal Jimenez ◽  
Hans J. Nelis
Keyword(s):  
Reactive Oxygen Species ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Membrane Perturbation ◽  
In Vitro Inhibition ◽  
Subinhibitory Concentrations

ABSTRACT We report that certain anthraquinones (AQs) reduce Streptococcus mutans biofilm formation on hydroxyapatite at concentrations below the MIC. Although AQs are known to generate reactive oxygen species, the latter do not underlie the observed effect. Our results suggest that AQs inhibit S. mutans biofilm formation by causing membrane perturbation.

scholarly journals A REVIEW ON ANTIOXIDANT METHODS

2016 ◽  
pp. 14 ◽  
Author(s):  
Dontha Sunitha
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Oxygen Species ◽  
Antioxidant Assay ◽  
Antioxidant Effects ◽  
In Vitro Antioxidant Activity

<p>ABSTRACT<br />To provide an outlook of the various available methods of antioxidant activity. Various available in vitro and in vivo methods are listed and the<br />procedure to perform the method, its mechanism is also explained in brief. 1,1-diphenyl-2-picrylhydrazyl method was found to be used mostly for the<br />in vitro antioxidant activity evaluation purpose while lipid peroxidation was found as mostly used in vivo antioxidant assay. An ethanol was with the<br />highest frequency as a solvent for extraction purpose. Summarized information on the various methods available provides with reliable information<br />to confirm the benefits of antioxidant effects.<br />Keywords: Antioxidant activity, Reactive oxygen species, Free radical, 1,1-diphenyl-2-picrylhydrazyl, Flavonoid.</p>

Scavenging of Reactive Oxygen Species by a Urinary Preparation

2000 ◽  
Vol 28 (02) ◽  
pp. 251-258 ◽  
Author(s):  
Wen-Chuan Lin ◽  
Tung-Yuan Lai ◽  
Yuen-Wern Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Anion Radical ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Order Rate Constant ◽  
Inhibitory Effect ◽  
Oxygen Species ◽  
Oxide Anion Radical

In the present paper, the antioxidant properties of a preparation of human urine (PHU) were evaluated by studying the ability of this drug to react with relevant biological oxidants such as super-oxide anion radical ( O 2·-) and hydroxyl radical ( OH ·). In addition, its effect on lipid peroxidation was investigated in vitro and ex vivo. PHU is not a good scavenger of O2·-. However, it react rapidly with OH· radicals with a second-order rate constant of 2.8 × 109/M/sec. The studies on rat brain homogenates showed that PHU had an inhibitory effect, which was dependent on its concentration and the magnitude of lipid peroxidation. Ex vivo studies also showed that oral administration of PHU increased the antioxidant capacity of plasma from rats. The ability of PHU to scavenge free radicals suggests that this drug may be potentially useful in counteracting free radical-mediated diseases.

scholarly journals In vitro inhibition of lipid peroxidation in fish by turmeric (Curcuma longa)

2006 ◽  
Vol 21 (2) ◽  
pp. 138-141 ◽  
Author(s):  
Hilda Priya D'Souza ◽  
H. Ramachandra Prabhu
Keyword(s):  
Lipid Peroxidation ◽  
Curcuma Longa ◽  
In Vitro Inhibition

scholarly journals Production of White, Red and Black Quinoa (Chenopodium quinoa Willd Var. Real) Protein Isolates and Its Hydrolysates in Germinated and Non-Germinated Quinoa Samples and Antioxidant Activity Evaluation

Plants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 257 ◽  
Author(s):  
Lucrecia Piñuel ◽  
Patricia Boeri ◽  
Fanny Zubillaga ◽  
Daniel Alejandro Barrio ◽  
Joaquin Torreta ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Zebrafish Embryo ◽  
Chenopodium Quinoa ◽  
Protein Isolate ◽  
Isoelectric Precipitation ◽  
Black And White ◽  
A Value ◽  
2S Albumins ◽  
Precipitation Ph

Red, black and white seeds quinoa were germinated at 28 °C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.

scholarly journals Impact of Simulated Gastrointestinal Conditions on Antiglycoxidant and α-Glucosidase Inhibition Capacities of Cyanidin-3-O-Glucoside

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1670
Author(s):  
Didier Fraisse ◽  
Alexis Bred ◽  
Catherine Felgines ◽  
François Senejoux
Keyword(s):  
Diabetes Management ◽  
Biological Activities ◽  
Inhibition Activity ◽  
Intestinal Phase ◽  
Simulated Digestion ◽  
Gastrointestinal Conditions ◽  
Physiological Relevance ◽  
Chemical Integrity ◽  
Hplc Analyses

Cyanidin-3-O-glucoside (C3G) is a widespread anthocyanin derivative, which has been reported in vitro to exert potent antioxidant, antiglycation and α-glucosidase inhibition effects. Nevertheless, the physiological relevance of such properties remains uncertain considering its significant instability in gastrointestinal conditions. A simulated digestion procedure was thus instigated to assess the influence of gastric and intestinal media on its chemical integrity and biological activities. HPLC analyses of digested C3G samples confirmed the striking impact of intestinal conditions, as attested by a decomposition ratio of 70%. In contrast, with recovery rates of around 90%, antiglycation, as well as DPPH and ABTS scavenging assays, uniformly revealed a noteworthy persistence of its antiglycoxidant capacities. Remarkably, a prominent increase of its α-glucosidase inhibition activity was even observed after the intestinal phase, suggesting that classical in vitro evaluations might underestimate C3G antidiabetic potential. Consequently, the present data provide novel and specific insights on C3G’s digestive fate, suggesting that the gastrointestinal tract does not profoundly affect its positive action on oxidative and carbonyl stresses. More specifically, it also tends to support its regulating effects on postprandial hyperglycemia and its potential usefulness for diabetes management.

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