Microvasculature of bone III microvasculature of the bone marrow of the rat's femur by means of vascular corrosion-cast SEM method

1989 ◽  
Vol 7 (2) ◽  
pp. 13-17
Author(s):  
Fumihiko Iwaku
Keyword(s):  
Bone Marrow ◽  
Vascular Corrosion Cast ◽  
Corrosion Cast

scholarly journals Effects of curcumin on testis microvascular complication in streptozotocin-induced diabetes rats

2019 ◽  
Vol 9 (1) ◽  
pp. 34
Author(s):  
Wipapan Khimmaktong ◽  
Manaras Komolkriengkrai ◽  
Piyakorn Boonyoung ◽  
Vipavee Anupunpisit
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Structural Changes ◽  
Testicular Tissue ◽  
Diabetic Rats ◽  
Seminiferous Tubules ◽  
Vascular Corrosion Cast ◽  
Pampiniform Plexus ◽  
Corrosion Cast ◽  
Scanning Electron

Background:  Diabetes has a significant impact on the impaired function of the reproductive system in the testis.Objective: The aim of this study was to investigate the effects of curcumin from turmeric on structural changes of testicular tissues and microvasculature in STZ-induced diabetic rats.Methods: Rats were injected with a single dose of streptozotocin (60 mg/kg BW) to induce a diabetic condition. Three days after the injection, rats with a blood sugar level > 250 mg/dl were considered diabetic. Rats were allocated to one of three groups, diabetic (DM), diabetic rats receiving curcumin (DMC) (200 mg/kg BW), and control (C). Testicular tissues were studied eight weeks after the treatment using the histological technique. Testicular microvasculature was examined using the vascular corrosion cast technique under a scanning electron microscope (SEM).Results: The testicular tissue of DM group showed damaged seminiferous tubules, spermatocytes, and spermatids with necrosis and germ cells degeneration. Destruction of testicular arteries and veins in pampiniform plexus were demonstrated. The destroyed capillaries, arterioles, and venules demonstrated stenosis and shrinkage. The vessel diameter in all DM groups decreased. In the DMC group which received curcumin, the appearance of all seminiferous tubules, spermatogenic, and sertoli cell was normal. The reconstruction and restoration of testicular artery, pampiniform plexus, capillary network, arterioles, and venules were clear. As a result of curcumin supplementation, the standard and healthy testis microvasculature was restored. The DMC vessels diameter increased back to a quasi normal condition, particularly at the artery, arteriole, and capillary levels.Conclusions: The results provided evident for effects of curcumin could be associated with recovering and improvement of the testicular tissues and microvasculatures in diabetic condition.Keywords: Diabetes mellitus, Streptozotocin, Curcumin, Testis, Microvasculature, Vascular corrosion cast, Scanning electron microscope

Placental vascular corrosion cast studies: A comparison between ruminants and humans

1997 ◽  
Vol 38 (1-2) ◽  
pp. 76-87 ◽  
Author(s):  
Rudolf Leiser ◽  
Christiane Krebs ◽  
Brigitte Ebert ◽  
Vibeke Dantzer
Keyword(s):  
Vascular Corrosion Cast ◽  
Corrosion Cast

From Vascular Corrosion Cast to Electrical Analog Model for the Study of Human Liver Hemodynamics and Perfusion

2011 ◽  
Vol 58 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Charlotte Debbaut ◽  
Diethard Monbaliu ◽  
Christophe Casteleyn ◽  
Pieter Cornillie ◽  
Denis Van Loo ◽  
...  
Keyword(s):  
Human Liver ◽  
Analog Model ◽  
Electrical Analog ◽  
Vascular Corrosion Cast ◽  
Corrosion Cast ◽  
Liver Hemodynamics

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

Mammalian Bone Marrow Acetylcholinesterase

1982 ◽  
Vol 40 ◽  
pp. 44-45
Author(s):  
Ezzatollah Keyhani
Keyword(s):  
Central Nervous System ◽  
Bone Marrow ◽  
Nervous System ◽  
Common Property ◽  
Extracellular Medium ◽  
The Central Nervous System ◽  
The Common ◽  
Mammalian Bone

Acetylcholinesterase (EC 3.1.1.7) (ACHE) has been localized at cholinergic junctions both in the central nervous system and at the periphery and it functions in neurotransmission. ACHE was also found in other tissues without involvement in neurotransmission, but exhibiting the common property of transporting water and ions. This communication describes intracellular ACHE in mammalian bone marrow and its secretion into the extracellular medium.

The structure of pre-mRNA and mRNA from erythroid cells

1978 ◽  
Vol 36 (2) ◽  
pp. 234-235
Author(s):  
A.-M. Ladhoff ◽  
B.J. Thiele ◽  
Ch. Coutelle ◽  
S. Rosenthal
Keyword(s):  
Bone Marrow ◽  
Structural Transformation ◽  
Electron Micrographs ◽  
Ammonium Acetate ◽  
Bone Marrow Cells ◽  
Erythroid Cells ◽  
Globin Mrna ◽  
Ordered Structures ◽  
Rabbit Bone ◽  
Rabbit Reticulocytes

The suggested precursor-product relationship between the nuclear pre-mRNA and the cytoplasmic mRNA has created increased interest also in the structure of these RNA species. Previously we have been published electron micrographs of individual pre-mRNA molecules from erythroid cells. An intersting observation was the appearance of a contour, probably corresponding to higher ordered structures, on one end of 10 % of the pre-mRNA molecules from erythroid rabbit bone marrow cells (Fig. 1A). A virtual similar contour was observed in molecules of 9S globin mRNA from rabbit reticulocytes (Fig. 1B). A structural transformation in a linear contour occurs if the RNA is heated for 10 min to 90°C in the presence of 80 % formamide. This structural transformation is reversible when the denatured RNA is precipitated and redissolved in 0.2 M ammonium acetate.

Sign in / Sign up

Export Citation Format

Share Document