Experimental determination of the operative parameters for a double antibody solid phase radioimmunoassay of luteinizing and follicle-stimulating hormones in serum

1975 ◽  
Vol 5 (3) ◽  
Author(s):  
Claudio Dotti ◽  
Giordano Filippi ◽  
Claudio Castagnetti ◽  
Riccardo Franchini
Keyword(s):  
Experimental Determination ◽  
Solid Phase ◽  
Double Antibody ◽  
Solid Phase Radioimmunoassay ◽  
Follicle Stimulating Hormones

Solid-phase radioimmunoassay for morphine, with use of an affinity-purified morphine antibody.

1978 ◽  
Vol 24 (2) ◽  
pp. 339-342 ◽  
Author(s):  
M Steiner ◽  
J L Spratt
Keyword(s):  
Affinity Chromatography ◽  
Correlation Coefficient ◽  
Solid Phase ◽  
Rapid Determination ◽  
Biological Fluids ◽  
Inhibitory Effect ◽  
Major Metabolite ◽  
Solid Phase Radioimmunoassay ◽  
Related Compounds

Abstract Morphine antibody purified by affinity chromatography was used to develop a solid-phase radioimmunoassay for morphine in polystyrene tubes. The tubes are coated with an appropriate concentration of the purified antibody, rinsed three times with buffered saline, and stored at -15 degrees C. Using tritiated dihydromorphine, we determined competitive morphine binding by difference when the radioactivity in the assay supernates was measured after incubation (1 h, 37 degrees C). Five standard curves, with use of serum equivalents of morphine ranging from 0 to 6 mug/liter, were linear and had a mean correlation coefficient of 0.98. Uncer conditions of the assay, levorphanol was comparable to morphine in its inhibitory effect on binding of labeled dihydromorphine, whereas dextrorphan was essentially inactive. Morphine-3-glucuronide, a major metabolite, is 55-fold less inhibitory in terms of its capacity to displace the radiolabel. We believe that the sensitivity of the technique, coupled with the simplicity of nonseparatory sampling, renders the system suitable for rapid determination of morphine and related compounds in biological fluids.

POLY-MALEIC ACID ANHYDRIDE (PMA) COUPLED CORTISOL ANTIBODY IN ROUTINE DETERMINATION OF URINARY FREE CORTISOL

1979 ◽  
Vol 91 (4) ◽  
pp. 680-691 ◽  
Author(s):  
Jan Nielsen
Keyword(s):  
Bovine Serum Albumin ◽  
Maleic Acid ◽  
Methylene Chloride ◽  
Solid Phase ◽  
Urinary Free Cortisol ◽  
Acid Anhydride ◽  
Age Difference ◽  
Free Cortisol ◽  
Solid Phase Radioimmunoassay

ABSTRACT Antibodies against cortisol-21-hemisuccinate conjugated to bovine serum albumin was raised in rabbits and conjugated with poly-maleic acid anhydride. The conjugate was used in a solid phase radioimmunoassay to measure the urinary excretion of unconjugated cortisol. Cortisol was extracted with methylene chloride, the extract purified by chromatography on Sephadex LH-20, and the cortisol content measured with the radioimmunoassay. The intra-assay variation of the method was 6 per cent and the interassay variation 10 per cent. In 95 normal adult persons the excretion of free cortisol was 11–70, mean 30 μg/24 h. No sex or age difference was found. In 8 children aged 7–14 years the excretion was 8–46 μg/24 h.

scholarly journals A Rapid Solid-Phase Radioimmunoassay for Human Plasma Follicle-Stimulating Hormone

1980 ◽  
Vol 17 (1) ◽  
pp. 38-44
Author(s):  
AC Lovesey
Keyword(s):  
Human Plasma ◽  
High Precision ◽  
Reference Values ◽  
Plasma Levels ◽  
Solid Phase ◽  
Follicle Stimulating Hormone ◽  
Diagnosis And Management ◽  
Double Antibody ◽  
Solid Phase Radioimmunoassay ◽  
Stimulating Hormone

The measurement of plasma levels of human follicle-stimulating hormone (FSH) has proved to be of value for the study of the hypothalamic-hypophyseal-gonadal axis, greatly facilitating the diagnosis and management of problems relating to the menopause and infertility. In the present work a solid-phase radioimmunoassay for human FSH has been developed. This system is characterised by high precision, is economical, and is considerably faster to operate than conventional double antibody systems used in the hospital assay service. Reference values for plasma FSH in various endocrine states are recorded and discussed.

Development of a Solid-Phase Radioimmunoassay for the Determination of Arginine-Vasopressin in Urine

1985 ◽  
Vol 6 (3) ◽  
pp. 207-225 ◽  
Author(s):  
P. Larose ◽  
J. Gutkowska ◽  
P. du Souich ◽  
H. Ong
Keyword(s):  
Arginine Vasopressin ◽  
Solid Phase ◽  
Solid Phase Radioimmunoassay

Basal levels of prolactin in goat blood measured throughout a 24-h period by a rapid double antibody–solid phase radioimmunoassay

1973 ◽  
Vol 40 (2) ◽  
pp. 235-245 ◽  
Author(s):  
I. C. Hart
Keyword(s):  
Solid Phase ◽  
Virgin Female ◽  
Double Antibody ◽  
Lactating Goats ◽  
Highly Sensitive ◽  
Solid Phase Radioimmunoassay ◽  
Circulating Levels

SummaryThe development of a rapid, highly sensitive double antibody–solid phase (DASP) radioimmunoassay for ovine/caprine prolactin is described. The assay was applied to the measurement of basal levels of prolactin in the blood of 4 anoestrous lactating female, 4 anoestrous virgin female and 4 castrated male goats. For several hours after milking there was a chronic gradually decreasing release of prolactin in the lactating goats, such that the circulating levels of prolactin were, for 14–16 h of the day, at a much higher level than those in the virgin female or castrated male goats. The basal levels of prolactin in the castrated male goats (87–200ng/ml) were somewhat higher than those in the virgin females (47–77ng/ml). The effects of feeding and stress on prolactin levels are discussed.

Solid phase radioimmunoassay for determination of conjugated cholic acid in serum

1976 ◽  
Vol 73 (2) ◽  
pp. 277-283 ◽  
Author(s):  
J.W.O. Van Den Berg ◽  
M. Van Blankenstein ◽  
Ellen P. Bosman-Jacobs ◽  
M. Frenkel ◽  
P. Hörchner ◽  
...  
Keyword(s):  
Cholic Acid ◽  
Solid Phase ◽  
Solid Phase Radioimmunoassay
Sign in / Sign up

Export Citation Format

Share Document