Double antibody solid-phase radioimmunoassay for staphylococcal enterotoxin A

1977 ◽  
Vol 4 (2) ◽  
pp. 137-143 ◽  
Author(s):  
S. Lindroth ◽  
A. Niskanen
Keyword(s):  
Solid Phase ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Double Antibody ◽  
Solid Phase Radioimmunoassay

scholarly journals Rapid Solid-Phase Radioimmunoassay for Staphylococcal Enterotoxin A

1973 ◽  
Vol 25 (5) ◽  
pp. 774-777 ◽  
Author(s):  
William S. Collins ◽  
Anna D. Johnson ◽  
Joseph F. Metzger ◽  
Reginald W. Bennett
Keyword(s):  
Solid Phase ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Solid Phase Radioimmunoassay

scholarly journals Rapid Solid-Phase Radioimmunoassay for Staphylococcal Enterotoxin A

1973 ◽  
Vol 25 (5) ◽  
pp. 774-777
Author(s):  
William S. Collins ◽  
Anna D. Johnson ◽  
Joseph F. Metzger ◽  
Reginald W. Bennett
Keyword(s):  
Solid Phase ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Solid Phase Radioimmunoassay

scholarly journals Staphylococcal Enterotoxin C: Solid-Phase Radioimmunoassay

1975 ◽  
Vol 30 (4) ◽  
pp. 700-701
Author(s):  
Joann A. Bukovic ◽  
Howard M. Johnson
Keyword(s):  
Solid Phase ◽  
Staphylococcal Enterotoxin ◽  
Solid Phase Radioimmunoassay

scholarly journals A Rapid Solid-Phase Radioimmunoassay for Human Plasma Follicle-Stimulating Hormone

1980 ◽  
Vol 17 (1) ◽  
pp. 38-44
Author(s):  
AC Lovesey
Keyword(s):  
Human Plasma ◽  
High Precision ◽  
Reference Values ◽  
Plasma Levels ◽  
Solid Phase ◽  
Follicle Stimulating Hormone ◽  
Diagnosis And Management ◽  
Double Antibody ◽  
Solid Phase Radioimmunoassay ◽  
Stimulating Hormone

The measurement of plasma levels of human follicle-stimulating hormone (FSH) has proved to be of value for the study of the hypothalamic-hypophyseal-gonadal axis, greatly facilitating the diagnosis and management of problems relating to the menopause and infertility. In the present work a solid-phase radioimmunoassay for human FSH has been developed. This system is characterised by high precision, is economical, and is considerably faster to operate than conventional double antibody systems used in the hospital assay service. Reference values for plasma FSH in various endocrine states are recorded and discussed.

Basal levels of prolactin in goat blood measured throughout a 24-h period by a rapid double antibody–solid phase radioimmunoassay

1973 ◽  
Vol 40 (2) ◽  
pp. 235-245 ◽  
Author(s):  
I. C. Hart
Keyword(s):  
Solid Phase ◽  
Virgin Female ◽  
Double Antibody ◽  
Lactating Goats ◽  
Highly Sensitive ◽  
Solid Phase Radioimmunoassay ◽  
Circulating Levels

SummaryThe development of a rapid, highly sensitive double antibody–solid phase (DASP) radioimmunoassay for ovine/caprine prolactin is described. The assay was applied to the measurement of basal levels of prolactin in the blood of 4 anoestrous lactating female, 4 anoestrous virgin female and 4 castrated male goats. For several hours after milking there was a chronic gradually decreasing release of prolactin in the lactating goats, such that the circulating levels of prolactin were, for 14–16 h of the day, at a much higher level than those in the virgin female or castrated male goats. The basal levels of prolactin in the castrated male goats (87–200ng/ml) were somewhat higher than those in the virgin females (47–77ng/ml). The effects of feeding and stress on prolactin levels are discussed.

Double-antibody radioimmunoassay of thyroid microsomal antibody in serum.

1981 ◽  
Vol 27 (1) ◽  
pp. 39-42 ◽  
Author(s):  
V T Kung ◽  
P M Weber ◽  
L V dos Remedios
Keyword(s):  
Solid Phase ◽  
Sodium Deoxycholate ◽  
Published Data ◽  
Health Examination ◽  
Standard Curve ◽  
Double Antibody ◽  
Microsomal Antibody ◽  
Solid Phase Radioimmunoassay ◽  
Chloramine T ◽  
Antimicrosomal Antibody

Abstract We describe a double-antibody radioimmunoassay for antimicrosomal antibodies in serum. Antigen in centrifuged pellets of microsome is solubilized by treatment with sodium deoxycholate solution and radiolabeled (125I] by the Chloramine T method. A 10-microL aliquot of human serum or antimicrosomal antibody standard is incubated overnight at room temperature with 125I-labeled microsome. Bound radiolabeled microsome is separated by precipitation with goat antihuman IgG antiserum. The percentage of 125I-labeled microsome bound by each serum is calculated, and its concentration of antimicrosomal antibody interpolated from a standard curve. The prevalence of abnormal antimicrosomal antibody was 16% in a self-referred asymptomatic or not acutely ill group of patients having a periodic multiphasic health examination. Values for patients with certain thyroid diseases generally agreed with other published data. We conclude that our assay is sensitive, precise, and accurate, and is more efficient for routine measurement of antimicrosomal antibody than the currently used solid-phase radioimmunoassay.

scholarly journals Assay of Australia Antigen Employing Double-Antibody and Solid-Phase Radioimmunoassay Techniques

Kanzo ◽  
1974 ◽  
Vol 15 (7) ◽  
pp. 427-432
Author(s):  
Shinichi KAKUMU ◽  
Shozo ITO ◽  
Sumihiko OKUYAMA ◽  
Seikichi INADA ◽  
Katsuhiko TAGO
Keyword(s):  
Solid Phase ◽  
Australia Antigen ◽  
Double Antibody ◽  
Solid Phase Radioimmunoassay

scholarly journals Staphylococcal Enterotoxin B: Solid-Phase Radioimmunoassay

1971 ◽  
Vol 22 (5) ◽  
pp. 837-841
Author(s):  
Howard M. Johnson ◽  
Joann A. Bukovic ◽  
Peter E. Kauffman ◽  
J. T. Peeler
Keyword(s):  
Solid Phase ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Solid Phase Radioimmunoassay ◽  
Enterotoxin B
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