scholarly journals Determination of insulin specific IgE in serum of diabetic patients by solid-phase radioimmunoassay

Diabetologia ◽  
1982 ◽  
Vol 22 (4) ◽  
pp. 254-257 ◽  
Author(s):  
K. Falholt
Keyword(s):  
Solid Phase ◽  
Specific Ige ◽  
Diabetic Patients ◽  
Solid Phase Radioimmunoassay

Solid-phase radioimmunoassay for morphine, with use of an affinity-purified morphine antibody.

1978 ◽  
Vol 24 (2) ◽  
pp. 339-342 ◽  
Author(s):  
M Steiner ◽  
J L Spratt
Keyword(s):  
Affinity Chromatography ◽  
Correlation Coefficient ◽  
Solid Phase ◽  
Rapid Determination ◽  
Biological Fluids ◽  
Inhibitory Effect ◽  
Major Metabolite ◽  
Solid Phase Radioimmunoassay ◽  
Related Compounds

Abstract Morphine antibody purified by affinity chromatography was used to develop a solid-phase radioimmunoassay for morphine in polystyrene tubes. The tubes are coated with an appropriate concentration of the purified antibody, rinsed three times with buffered saline, and stored at -15 degrees C. Using tritiated dihydromorphine, we determined competitive morphine binding by difference when the radioactivity in the assay supernates was measured after incubation (1 h, 37 degrees C). Five standard curves, with use of serum equivalents of morphine ranging from 0 to 6 mug/liter, were linear and had a mean correlation coefficient of 0.98. Uncer conditions of the assay, levorphanol was comparable to morphine in its inhibitory effect on binding of labeled dihydromorphine, whereas dextrorphan was essentially inactive. Morphine-3-glucuronide, a major metabolite, is 55-fold less inhibitory in terms of its capacity to displace the radiolabel. We believe that the sensitivity of the technique, coupled with the simplicity of nonseparatory sampling, renders the system suitable for rapid determination of morphine and related compounds in biological fluids.

POLY-MALEIC ACID ANHYDRIDE (PMA) COUPLED CORTISOL ANTIBODY IN ROUTINE DETERMINATION OF URINARY FREE CORTISOL

1979 ◽  
Vol 91 (4) ◽  
pp. 680-691 ◽  
Author(s):  
Jan Nielsen
Keyword(s):  
Bovine Serum Albumin ◽  
Maleic Acid ◽  
Methylene Chloride ◽  
Solid Phase ◽  
Urinary Free Cortisol ◽  
Acid Anhydride ◽  
Age Difference ◽  
Free Cortisol ◽  
Solid Phase Radioimmunoassay

ABSTRACT Antibodies against cortisol-21-hemisuccinate conjugated to bovine serum albumin was raised in rabbits and conjugated with poly-maleic acid anhydride. The conjugate was used in a solid phase radioimmunoassay to measure the urinary excretion of unconjugated cortisol. Cortisol was extracted with methylene chloride, the extract purified by chromatography on Sephadex LH-20, and the cortisol content measured with the radioimmunoassay. The intra-assay variation of the method was 6 per cent and the interassay variation 10 per cent. In 95 normal adult persons the excretion of free cortisol was 11–70, mean 30 μg/24 h. No sex or age difference was found. In 8 children aged 7–14 years the excretion was 8–46 μg/24 h.

Hyperlabile diabetes accompanied by insulin resistance.

1981 ◽  
Vol 27 (8) ◽  
pp. 1463-1464 ◽  
Author(s):  
A Renie ◽  
R G Hamilton ◽  
N F Adkinson ◽  
M S Rendell
Keyword(s):  
Insulin Resistance ◽  
Solid Phase ◽  
Insulin Requirement ◽  
Porcine Insulin ◽  
Insulin Antibody ◽  
Insulin Dosage ◽  
Diabetic Patients ◽  
Free Insulin ◽  
High Insulin ◽  
Solid Phase Radioimmunoassay

Abstract It is generally held that high insulin antibody concentrations, by "buffering" abrupt swings in free insulin concentrations after injections of exogenous insulin, tend to stabilize blood glucose variations in diabetic patients. However, we encountered a patient with extremely labile diabetes coexisting with insulin resistance. This patient's injections were switched to pure porcine insulin from his usual mixed bovine/porcine insulin, in an effort to decrease his insulin requirement. This treatment was successful, and, as his insulin dosage decreased, his diabetic lability diminished substantially. His diabetes was eventually considered stabilized on about 22 units of porcine insulin daily. The serial decrease in his insulin antibody concentrations, monitored by use of solid-phase radioimmunoassay, paralleled the disappearance of his diabetic lability as well as the decrease in his insulin requirement.

Development of a Solid-Phase Radioimmunoassay for the Determination of Arginine-Vasopressin in Urine

1985 ◽  
Vol 6 (3) ◽  
pp. 207-225 ◽  
Author(s):  
P. Larose ◽  
J. Gutkowska ◽  
P. du Souich ◽  
H. Ong
Keyword(s):  
Arginine Vasopressin ◽  
Solid Phase ◽  
Solid Phase Radioimmunoassay

Solid phase radioimmunoassay for determination of conjugated cholic acid in serum

1976 ◽  
Vol 73 (2) ◽  
pp. 277-283 ◽  
Author(s):  
J.W.O. Van Den Berg ◽  
M. Van Blankenstein ◽  
Ellen P. Bosman-Jacobs ◽  
M. Frenkel ◽  
P. Hörchner ◽  
...  
Keyword(s):  
Cholic Acid ◽  
Solid Phase ◽  
Solid Phase Radioimmunoassay

scholarly journals Determination of human immunoglobulin M rheumatoid factor by a solid-phase radioimmunoassay which uses human immunoglobulin G in antigen-antibody complexes

1978 ◽  
Vol 8 (2) ◽  
pp. 134-141
Author(s):  
B Ziola ◽  
O Meurman ◽  
M T Matikainen ◽  
A Salmi ◽  
J L Kalliomäki
Keyword(s):  
Rheumatoid Factor ◽  
Solid Phase ◽  
Rapid Determination ◽  
Physiological Role ◽  
Virus Antigen ◽  
Immunoglobulin M ◽  
Human Immunoglobulin ◽  
Solid Phase Radioimmunoassay ◽  
Syncytial Virus

A solid-phase radioimmunoassay for the rapid determination of human immunoglobulin M (IgM) rheumatoid factor (RF) has been developed. Preparation of the solid phase for the assay involved the formation of complexes between respiratory syncytial virus-specific human IgG antibodies and virus antigen on the surface of polystyrene balls. Binding of serum RF to IgG in the immune complex was subsequently detected by 125I-labeled mu-chain-specific antibodies to human IgM. The amount of radioactive indicator antibody bound was converted to units of RF by comparison to the standard curve for an RF reference-serum pool. This assay should prove useful in studies of the physiological role of RF, since it can effectively measure low levels of circulating RF.

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