Selection of auxotrophic mutants inSaccharomyces cerevisiae by a snail enzyme digestion method

1976 ◽  
Vol 21 (5) ◽  
pp. 337-341 ◽  
Author(s):  
D. Piedra ◽  
L. Herrera
Keyword(s):  
Enzyme Digestion ◽  
Digestion Method ◽  
Auxotrophic Mutants ◽  
Snail Enzyme ◽  
Selection Of

Optimization on the selection of auxotrophic mutants in Candida utilis by snail enzyme treatment

1979 ◽  
Vol 25 (4) ◽  
pp. 486-490 ◽  
Author(s):  
J. M. Delgado ◽  
L. S. Herrera ◽  
C. Pérez ◽  
R. López
Keyword(s):  
Candida Utilis ◽  
Wild Type Strain ◽  
Selection Procedure ◽  
Enzyme Treatment ◽  
Wild Type ◽  
Digestion Method ◽  
Auxotrophic Mutants ◽  
Snail Enzyme ◽  
Selection Of

The determination of the best conditions for the application of the snail enzyme digestion method in the enrichment of auxotrophic mutants in Candida utilis was carried out following Box and Wilson's mathematical method. The selection procedure proposed was tested in the enrichment of auxotrophic mutants from a mutagenized culture of a wild-type strain. Mutant frequency was increased 46-fold by treatment with snail enzyme. The method also proved useful in the selection of additional auxotrophic mutations from single auxotrophs.

scholarly journals Enhancement Of Dermal Fibroblast Isolation Method

2015 ◽  
Vol 16 (1) ◽  
pp. 65-69
Author(s):  
Milan Zaric ◽  
Ivana Nikolic ◽  
Ivanka Zelen ◽  
Marina Mitrovic ◽  
Zoran Milosavljevic
Keyword(s):  
Dermal Fibroblast ◽  
Petri Dish ◽  
Culture Conditions ◽  
Dermal Fibroblasts ◽  
Enzyme Digestion ◽  
Digestion Method ◽  
Primary Isolation ◽  
Donor Cells ◽  
Cell Culture Conditions

ABSTRACTCultivated fibroblasts have been widely used in a large number of in vitro studies. Although they readily proliferate under cell culture conditions, improvements in methods for their isolation are necessary. Here, we present our modified enzyme digestion method and compare its efficiency with commonly used techniques.Three foreskin samples from young, middle-aged and old donors were used. The classical explant, standard enzyme digestion method with collagenase and our improved enzyme digestion method were compared for efficiency of fibroblast isolation and the time needed to achieve 95% confluence in a 30-mm Petri dish.The explant method was the slowest to achieve fibroblast confluence, especially with the tissues from the older donors (up to 23 days). With the standard enzyme digestion method, the skin tissue was partially digested, but the fibroblasts reached confluence much faster (the younger donor cells needed approximately 7 days to reach confluence). Our modified “mixed” enzyme digestion method was the fastest (the fibroblasts from the younger donors needed up to 5 days to reach confluence).For studies requiring the primary isolation and cultivation of dermal fibroblasts, the best method to achieve this goal is the tissue digestion method with the multiple enzyme solution.

Application of an enzyme digestion method reveals microlitter in Mytilus trossulus at a wastewater discharge area

2018 ◽  
Vol 130 ◽  
pp. 206-214 ◽  
Author(s):  
Saana Railo ◽  
Julia Talvitie ◽  
Outi Setälä ◽  
Arto Koistinen ◽  
Maiju Lehtiniemi
Keyword(s):  
Enzyme Digestion ◽  
Mytilus Trossulus ◽  
Wastewater Discharge ◽  
Digestion Method ◽  
Discharge Area

scholarly journals Production of auxotrophic mutants in ferns

1969 ◽  
Vol 14 (3) ◽  
pp. 337-339 ◽  
Author(s):  
Peter S. Carlson
Keyword(s):  
Higher Plants ◽  
Auxotrophic Mutants ◽  
Wide Range ◽  
Selection Of

A method has been devised for the selection of auxotrophic mutants in higher plants. The method depends upon the incorporation of BUdR into the DNA of non-auxotrophic cells and upon its lack of incorporation into the DNA of auxotrophic cells. A wide range of auxotrophic types were recovered.

scholarly journals Selection of auxotrophic mutants and heterokaryosis in Alternaria alternata.

1987 ◽  
Vol 53 (2) ◽  
pp. 182-190 ◽  
Author(s):  
Takashi TSUGE ◽  
Noriki HAYASHI ◽  
Syoyo NISHIMURA
Keyword(s):  
Alternaria Alternata ◽  
Auxotrophic Mutants ◽  
Selection Of

scholarly journals Evaluation of Transcriptional Efficiency of Hepatitis B Virus Covalently Closed Circular DNA by Reverse Transcription-PCR Combined with the Restriction Enzyme Digestion Method

2005 ◽  
Vol 79 (3) ◽  
pp. 1813-1823 ◽  
Author(s):  
Yu-Chi Chou ◽  
King-Song Jeng ◽  
Mong-Liang Chen ◽  
Hsiao-Hui Liu ◽  
Tzu-Ling Liu ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Hepatitis B ◽  
Transcriptional Activity ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Rt Pcr ◽  
Digestion Method ◽  
Circular Dna ◽  
Reverse Transcription Pcr ◽  
B Virus

ABSTRACT Virus persistence in chronic hepatitis B patients is due to the sustaining level of covalently closed circular DNA (cccDNA) within the nuclei of infected hepatocytes. In this study, we used a modified 1.3-fold hepatitis B virus (HBV) genome, with a BclI genetic marker embedded in the redundancy region, to examine the transcriptional activity of cccDNA and the effect of the HBx protein on transcriptional regulation. After harvesting total RNA from transfected cells or stable lines, we specifically identified and monitored the transcripts from cccDNA by using reverse transcription-PCR (RT-PCR) combined with the restriction enzyme digestion method. In this approach, we have found that (i) RT-PCR combined with detection of the BclI marker is a highly specific method for distinguishing cccDNA-derived transcripts from the original integrated viral genome, (ii) the transcriptional ability of cccDNA was less efficient than that from the integrated viral genome, and (iii) the transcriptional activity of cccDNA was significantly regulated by the HBx protein, a potential transcription activator. In conclusion, we provided a tool with which to elucidate the transcriptional regulation of cccDNA and clarified the transcriptional regulation mechanism of HBx on cccDNA. The results obtained may be helpful in the development of a clinical intervention for patients with chronic HBV infections.

Efficient Method for Selection of Auxotrophic Mutants of Neurospora

Science ◽  
1959 ◽  
Vol 129 (3348) ◽  
pp. 572-572 ◽  
Author(s):  
H. E. LESTER ◽  
S. R. GROSS
Keyword(s):  
Efficient Method ◽  
Auxotrophic Mutants ◽  
Selection Of
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