Larval development rate and mortality of Colorado potato beetle on detached leaves of wildSolanum species

1989 ◽  
Vol 66 (9) ◽  
pp. 575-582 ◽  
Author(s):  
L. L. Sanford ◽  
W. W. Cantelo
Keyword(s):  
Larval Development ◽  
Colorado Potato Beetle ◽  
Development Rate ◽  
Potato Beetle ◽  
Detached Leaves ◽  
Wildsolanum Species

scholarly journals Engineering Transgenic Eggplant (Solanum melongena L.) Resistant to the Colorado Potato Beetle (Leptinotarsa decemlineata Say)

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 572a-572
Author(s):  
G. Jelenkovic ◽  
S. Billings ◽  
Q. Chen ◽  
J. Lashomb ◽  
G. Ghidiu
Keyword(s):  
Transgenic Plants ◽  
Colorado Potato Beetle ◽  
Solanum Melongena ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
Detached Leaves ◽  
Chimeric Construct ◽  
Single Construct ◽  
High Level ◽  
Very High

A chimeric construct, containing the synthetic cryIIIA (Btt) gene, the NPTII selectable marker and the uidA reporter gene, was incorporated via Agrobacterium tumefaciens into eggplant, variety Hibush. The synthetic cryIIIA gene, altered at the nucleotide level without changing the amino acids of the toxic protein by J. Kemp of New Mexico State Univ., Las Cruces, is adapted for high expression in plant cells. To verify the transgenic status, GUS assays were performed on over 300 plants, from which 185 were confirmed to be transgenic. Physical incorporation of the chimeric construct was further confirmed by Southern analysis of about 30 transgenic plants; both single and multiple site incorporation of the Btt gene were found. Resistance to Colorado potato beetle (CPB) was assessed by: a) placing egg masses of CPB on leaves of plants grown in the growth chamber; b) placing first-instar larvae on detached leaves; c) observing 173 transgenic plants under field conditions. About 60% of the transgenic plants displayed a very high level of resistance to CPB. No larvae survived on the resistant plants longer than 50–60 hours after hatching. Upon selfing, the transgenic plants with a single construct segregate in the S1 generation in a Mendelian fashion.

Co-expression of the proteinase inhibitors oryzacystatin I and oryzacystatin II in transgenic potato alters Colorado potato beetle larval development

2016 ◽  
Vol 24 (5) ◽  
pp. 768-780 ◽  
Author(s):  
Aleksandar Cingel ◽  
Jelena Savić ◽  
Jelica Lazarević ◽  
Tatjana Ćosić ◽  
Martin Raspor ◽  
...  
Keyword(s):  
Larval Development ◽  
Colorado Potato Beetle ◽  
Proteinase Inhibitors ◽  
Transgenic Potato ◽  
Potato Beetle
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