Organization of actin filaments in regenerating and outgrowing subprotoplasts from pollen tubes ofNicotiana tabacum L.

Planta ◽  
1990 ◽  
Vol 180 (4) ◽  
pp. 471-479 ◽  
Author(s):  
Twan L. M. Rutten ◽  
Jan Derksen
Keyword(s):  
Actin Filaments ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum

Microtubules and actin filaments co-localize in pollen tubes ofNicotiana tabacum L. andLilium longiflorum Thunb.

PROTOPLASMA ◽  
1989 ◽  
Vol 150 (1) ◽  
pp. 75-77 ◽  
Author(s):  
Elisabeth S. Pierson ◽  
H. M. P. Kengen ◽  
J. Derksen
Keyword(s):  
Actin Filaments ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum

scholarly journals ADF10 shapes the overall organization of apical actin filaments by promoting their turnover and ordering in pollen tubes

2017 ◽  
Vol 130 (23) ◽  
pp. 3988-4001 ◽  
Author(s):  
Yuxiang Jiang ◽  
Juan Wang ◽  
Yurong Xie ◽  
Naizhi Chen ◽  
Shanjin Huang
Keyword(s):  
Actin Filaments ◽  
Pollen Tubes

Ultrastructural immunolocalization of periodic pectin depositions in the cell wall ofNicotiana tabacum pollen tubes

PROTOPLASMA ◽  
1995 ◽  
Vol 187 (1-4) ◽  
pp. 168-171 ◽  
Author(s):  
Anja Geitmann ◽  
Yi-Qin Li ◽  
M. Cresti
Keyword(s):  
Cell Wall ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum

Ultrastructure of sperm cells and the male germ unit in pollen tubes ofNicotiana tabacum

PROTOPLASMA ◽  
1989 ◽  
Vol 152 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Hong-Shi Yu ◽  
Shi-Yi Hu ◽  
Cheng Zhu
Keyword(s):  
Pollen Tubes ◽  
Sperm Cells ◽  
Male Germ Unit ◽  
Ofnicotiana Tabacum

Ca2+-induced fragmentation of actin filaments in pollen tubes

PROTOPLASMA ◽  
1987 ◽  
Vol 141 (2-3) ◽  
pp. 177-179 ◽  
Author(s):  
T. Kohno ◽  
T. Shimmen
Keyword(s):  
Actin Filaments ◽  
Pollen Tubes

Immunogold localization of arabinogalactan proteins, unesterified and esterified pectins in pollen grains and pollen tubes ofNicotiana tabacum L.

PROTOPLASMA ◽  
1995 ◽  
Vol 189 (1-2) ◽  
pp. 26-36 ◽  
Author(s):  
Yi -Qin Li ◽  
Claudia Faleri ◽  
Anja Geitmann ◽  
Hong -Qi Zhang ◽  
Mauro Cresti
Keyword(s):  
Pollen Grains ◽  
Pollen Tubes ◽  
Arabinogalactan Proteins ◽  
Immunogold Localization ◽  
Ofnicotiana Tabacum

scholarly journals Localization of actin in pollen tubes of Ornithogalum virens L.

2014 ◽  
Vol 68 (2) ◽  
pp. 97-102
Author(s):  
Małgorzata Stępka ◽  
Fabricio Ciampolini ◽  
Mauro Cresti ◽  
Maria Charzyńska
Keyword(s):  
Pollen Tube ◽  
Actin Filaments ◽  
Laser Scanning ◽  
Higher Plants ◽  
Generative Cell ◽  
Pollen Tubes ◽  
Confocal Laser ◽  
Vegetative Nucleus ◽  
Ornithogalum Virens

The germinating pollen grain (in vivo on the stigma or in vitro in germination medium) forms a pollen tube which transports the vegetative nucleus and generative cell/two sperm cells participating in the process of double fertilization. The growth of the tube and the transport of organelles and the cells occur due to two major motor systems existing in the pollen tubes of higher plants: the tubuline-dynein/kinesin and the actin-myosin system. In pollen tubes of <em>Ornithogalum virens</em> the actin filaments were labelled with TRITC-phalloidin (2 µg/ml) in the PIPES buffer and the 10% sucrose, without the fixative and DMSO. Omission of the fixative and permeabilizing agent (DMSO) allowed better preservation of the structure, and the "fluorescence" of actin was observed in living pollen tubes. Observations in CLSM (confocal laser scanning microscope) showed that actin is distributed in the vicinity of the cell membrane. This could support the view that actin filaments and the plasmalemma form the pollen tube cortex along which the cytoplasmic movement of organelles, and cell transport occurs.

Cytosolic proteins from tobacco pollen tubes that crosslink microtubules and actin filaments in vitro are metabolic enzymes

Cytoskeleton ◽  
2010 ◽  
Vol 67 (12) ◽  
pp. 745-754 ◽  
Author(s):  
Silvia Romagnoli ◽  
Claudia Faleri ◽  
Luca Bini ◽  
Tobias I. Baskin ◽  
Mauro Cresti
Keyword(s):  
Actin Filaments ◽  
Metabolic Enzymes ◽  
Pollen Tubes ◽  
Cytosolic Proteins ◽  
Tobacco Pollen
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