Immunoelectron microscopy of myosin associated with the generative cells in pollen tubes ofNicotiana tabacum L.

1996 ◽  
Vol 9 (4) ◽  
pp. 233-237 ◽  
Author(s):  
Uday K. Tirlapur ◽  
Claudia Faleri ◽  
Mauro Cresti
Keyword(s):  
Immunoelectron Microscopy ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum ◽  
Generative Cells

scholarly journals Identification and Characterization of Plasma Membrane Proteins that Bind to Microtubules in Pollen Tubes and Generative Cells of Tobacco

2005 ◽  
Vol 46 (4) ◽  
pp. 563-578 ◽  
Author(s):  
Giampiero Cai ◽  
Elisa Ovidi ◽  
Silvia Romagnoli ◽  
Marylin Vantard ◽  
Mauro Cresti ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Pollen Tubes ◽  
Plasma Membrane Proteins ◽  
Identification And Characterization ◽  
Generative Cells

Isolation of male and female gametes, zygotes and proembryos of leek (Allium tuberosum Roxb)

Zygote ◽  
2020 ◽  
Vol 28 (4) ◽  
pp. 278-285
Author(s):  
Yi Hua Lin ◽  
Mei Zhen Lin ◽  
Yu Qing Chen ◽  
Hui Qiao Tian
Keyword(s):  
Higher Plants ◽  
Osmotic Shock ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Development Stage ◽  
Male And Female ◽  
Allium Tuberosum ◽  
Biological Methods ◽  
Generative Cells

SummaryThe isolation of male and female gametes is an effective method to study the fertilization mechanisms of higher plants. An osmotic shock method was used to rupture pollen grains of Allium tuberosum Roxb and release the pollen contents, including generative cells, which were mass collected. The pollinated styles were cut following 3 h of in vivo growth, and cultured in medium for 6–8 h, during which time pollen tubes grew out of the cut end of the style. After pollen tubes were transferred into a solution containing 6% mannitol, tubes burst and released pairs of sperm cells. Ovules of A. tuberosum were incubated in an enzyme solution for 30 min, and then dissected to remove the integuments. Following transfer to a dissecting solution free of enzymes, each nucellus was cut in the middle, and squeezed gently on the micropylar end, resulting in the liberation of the egg, zygote and proembryo from ovules at selected stages. These cells can be used to explore fertilization and embryonic development using molecular biological methods for each cell type and development stage.

Microtubules and actin filaments co-localize in pollen tubes ofNicotiana tabacum L. andLilium longiflorum Thunb.

PROTOPLASMA ◽  
1989 ◽  
Vol 150 (1) ◽  
pp. 75-77 ◽  
Author(s):  
Elisabeth S. Pierson ◽  
H. M. P. Kengen ◽  
J. Derksen
Keyword(s):  
Actin Filaments ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum

Ultrastructural immunolocalization of periodic pectin depositions in the cell wall ofNicotiana tabacum pollen tubes

PROTOPLASMA ◽  
1995 ◽  
Vol 187 (1-4) ◽  
pp. 168-171 ◽  
Author(s):  
Anja Geitmann ◽  
Yi-Qin Li ◽  
M. Cresti
Keyword(s):  
Cell Wall ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum

Ultrastructure of sperm cells and the male germ unit in pollen tubes ofNicotiana tabacum

PROTOPLASMA ◽  
1989 ◽  
Vol 152 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Hong-Shi Yu ◽  
Shi-Yi Hu ◽  
Cheng Zhu
Keyword(s):  
Pollen Tubes ◽  
Sperm Cells ◽  
Male Germ Unit ◽  
Ofnicotiana Tabacum

Immunogold localization of arabinogalactan proteins, unesterified and esterified pectins in pollen grains and pollen tubes ofNicotiana tabacum L.

PROTOPLASMA ◽  
1995 ◽  
Vol 189 (1-2) ◽  
pp. 26-36 ◽  
Author(s):  
Yi -Qin Li ◽  
Claudia Faleri ◽  
Anja Geitmann ◽  
Hong -Qi Zhang ◽  
Mauro Cresti
Keyword(s):  
Pollen Grains ◽  
Pollen Tubes ◽  
Arabinogalactan Proteins ◽  
Immunogold Localization ◽  
Ofnicotiana Tabacum

scholarly journals Myosin associated with the surfaces of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes

1989 ◽  
Vol 94 (2) ◽  
pp. 319-325
Author(s):  
J. HESLOP-HARRISON ◽  
Y. HESLOP-HARRISON
Keyword(s):  
Pollen Tube ◽  
Pollen Grains ◽  
Giant Cells ◽  
Pollen Tubes ◽  
Microfilament Bundles ◽  
Hyacinthus Orientalis ◽  
Actin Fibrils ◽  
Angiosperm Pollen ◽  
Muscle Myosin ◽  
Generative Cells

Myosin, detected by immunofluorescence using an antibody to bovine skeletal and smooth muscle myosin, has been localised on individual identifiable organelles from the grasses Alopecurus pratensis and Secale cereale, and on the surfaces of vegetative nuclei and generative cells from pollen and pollen tubes of Hyacinthus orientalis and Helleborus foetidus. Taken in conjunction with recent evidence showing that the growing pollen tube contains an actin cytoskeleton consisting of numerous mainly longitudinally oriented microfilament bundles, and that isolated pollen-tube organelles show ATP-dependent movement along the actin bundles of the giant cells of the characeous algae, this finding suggests that an actomyosin motility system is present in pollen tubes, and indicates that the movements of the different classes of inclusions are driven by interaction of the surface myosin with the actin fibrils at the zones of contact.

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