Ultrastructural immunolocalization of periodic pectin depositions in the cell wall ofNicotiana tabacum pollen tubes

PROTOPLASMA ◽  
1995 ◽  
Vol 187 (1-4) ◽  
pp. 168-171 ◽  
Author(s):  
Anja Geitmann ◽  
Yi-Qin Li ◽  
M. Cresti
Keyword(s):  
Cell Wall ◽  
Pollen Tubes ◽  
Ofnicotiana Tabacum

scholarly journals Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides

2020 ◽  
Author(s):  
María Flores-Tornero ◽  
Lele Wang ◽  
David Potěšil ◽  
Said Hafidh ◽  
Frank Vogler ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Turgor Pressure ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Secreted Proteins ◽  
Reproductive Tissues ◽  
Small Proteins ◽  
Secreted Peptides

Abstract Key message Analyses of secretomes of in vitro grown pollen tubes from Amborella, maize and tobacco identified many components of processes associated with the cell wall, signaling and metabolism as well as novel small secreted peptides. Abstract Flowering plants (angiosperms) generate pollen grains that germinate on the stigma and produce tubes to transport their sperm cells cargo deep into the maternal reproductive tissues toward the ovules for a double fertilization process. During their journey, pollen tubes secrete many proteins (secreted proteome or secretome) required, for example, for communication with the maternal reproductive tissues, to build a solid own cell wall that withstands their high turgor pressure while softening simultaneously maternal cell wall tissue. The composition and species specificity or family specificity of the pollen tube secretome is poorly understood. Here, we provide a suitable method to obtain the pollen tube secretome from in vitro grown pollen tubes of the basal angiosperm Amborella trichopoda (Amborella) and the Poaceae model maize. The previously published secretome of tobacco pollen tubes was used as an example of eudicotyledonous plants in this comparative study. The secretome of the three species is each strongly different compared to the respective protein composition of pollen grains and tubes. In Amborella and maize, about 40% proteins are secreted by the conventional “classic” pathway and 30% by unconventional pathways. The latter pathway is expanded in tobacco. Proteins enriched in the secretome are especially involved in functions associated with the cell wall, cell surface, energy and lipid metabolism, proteolysis and redox processes. Expansins, pectin methylesterase inhibitors and RALFs are enriched in maize, while tobacco secretes many proteins involved, for example, in proteolysis and signaling. While the majority of proteins detected in the secretome occur also in pollen grains and pollen tubes, and correlate in the number of mapped peptides with relative gene expression levels, some novel secreted small proteins were identified. Moreover, the identification of secreted proteins containing pro-peptides indicates that these are processed in the apoplast. In conclusion, we provide a proteome resource from three distinct angiosperm clades that can be utilized among others to study the localization, abundance and processing of known secreted proteins and help to identify novel pollen tube secreted proteins for functional studies.

Periodic deposition of arabinogalactan epitopes in the cell wall of pollen tubes of Nicotiana tabacum L.

Planta ◽  
1992 ◽  
Vol 188 (4) ◽  
Author(s):  
Yi-qin Li ◽  
Lone Bruun ◽  
ElisabethS. Pierson ◽  
Mauro Cresti
Keyword(s):  
Cell Wall ◽  
Nicotiana Tabacum ◽  
Pollen Tubes ◽  
Nicotiana Tabacum L

Effets d'une plasmolyse prolongée sur les tubes polliniques angiospermiens en culture in vitro : étude ultrastructurale

1988 ◽  
Vol 66 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Jean-Claude Pargney
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Pollen Tubes ◽  
Ultrastructural Changes ◽  
Cell Wall Synthesis ◽  
Culture In Vitro

In angiosperm plants subjected to plasmolysis, pollen tubes may undergo substantial ultrastructural changes accompanied by a gradual deterioration of those processes involved in cell syntheses. However, some tubes quickly regenerate a polysaccharide wall and thus ensure their extension. Others undergo fragmentation of their cytoplasm and a serious breakdown in processes involved in cell wall synthesis. In these extreme cases, the endoplasmic reticulum is the only compartment that is readily discernible.

scholarly journals Mechanical properties of the stigmatic cell wall mediate pollen tube path in Arabidopsis

2018 ◽  
Author(s):  
Lucie Riglet ◽  
Frédérique Rozier ◽  
Chie Kodera ◽  
Isabelle Fobis-Loisy ◽  
Thierry Gaude
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Cell Shape ◽  
Cell Imaging ◽  
Pollen Tubes ◽  
Mechanical Anisotropy ◽  
Cellulose Microfibrils ◽  
Mechanical Forces ◽  
Cortical Microtubules ◽  
Isotropic Reorientation

ABSTRACTSuccessful fertilization in angiosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovules. Pollen tubes first grow within the cell wall of the papilla cells, applying pressure to the cell. Mechanical forces are known to play a major role in plant cell shape by controlling the orientation of cortical microtubules (CMTs), which in turn mediate deposition of cellulose microfibrils (CMFs). Here, by combining cell imaging and genetic approaches, we show that isotropic reorientation of CMTs and CMFs in aged and katanin1-5 (ktn1-5) papilla cells is accompanied by a tendency of pollen tubes to coil around the papillae. Furthermore, we uncover that aged and ktn1-5 papilla cells have a softer cell wall and provide less resistance to pollen tube growth. Our results reveal an unexpected role for KTN1 in pollen tube guidance by ensuring mechanical anisotropy of the papilla cell wall.

Cold stress affects cell wall deposition and growth pattern in tobacco pollen tubes

Plant Science ◽  
2019 ◽  
Vol 283 ◽  
pp. 329-342 ◽  
Author(s):  
Luigi Parrotta ◽  
Claudia Faleri ◽  
Gea Guerriero ◽  
Giampiero Cai
Keyword(s):  
Cell Wall ◽  
Cold Stress ◽  
Growth Pattern ◽  
Pollen Tubes ◽  
Wall Deposition ◽  
Tobacco Pollen ◽  
Cell Wall Deposition

scholarly journals Plant AP180 N-Terminal Homolog Proteins Are Involved in Clathrin-Dependent Endocytosis during Pollen Tube Growth in Arabidopsis thaliana

2019 ◽  
Vol 60 (6) ◽  
pp. 1316-1330 ◽  
Author(s):  
Minako Kaneda ◽  
Chlo� van Oostende-Triplet ◽  
Youssef Chebli ◽  
Christa Testerink ◽  
Sebastian Y Bednarek ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Membrane Trafficking ◽  
Pollen Tubes ◽  
Wall Material ◽  
Knockout Mutant ◽  
Cell Wall Assembly ◽  
Morphological Defects ◽  
Fluorescent Tagging

Abstract Polarized cell growth in plants is maintained under the strict control and exquisitely choreographed balance of exocytic and endocytic membrane trafficking. The pollen tube has become a model system for rapid polar growth in which delivery of cell wall material and membrane recycling are controlled by membrane trafficking. Endocytosis plays an important role that is poorly understood. The plant AP180 N-Terminal Homolog (ANTH) proteins are putative homologs of Epsin 1 that recruits clathrin to phosphatidylinositol 4, 5-bisphosphate (PIP2) containing membranes to facilitate vesicle budding during endocytosis. Two Arabidopsis ANTH encoded by the genes AtAP180 and AtECA2 are highly expressed in pollen tubes. Pollen tubes from T-DNA inserted knockout mutant lines display significant morphological defects and unique pectin deposition. Fluorescent tagging reveals organization into dynamic foci located at the lateral flanks of the pollen tube. This precisely defined subapical domain coincides which clathrin-mediated endocytosis (CME) and PIP2 localization. Using a liposome-protein binding test, we showed that AtECA2 protein and ANTH domain recombinant proteins have strong affinity to PIP2 and phosphatidic acid containing liposomes in vitro. Taken together these data suggest that Arabidopsis ANTH proteins may play an important role in CME, proper cell wall assembly and morphogenesis.

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