Immunological hystochemical characteristics of one of the organ-specific antigens of mouse liver

1963 ◽  
Vol 56 (5) ◽  
pp. 1265-1268
Author(s):  
N. V. Engel'gardt
Keyword(s):  
Mouse Liver ◽  
Organ Specific ◽  
Specific Antigens

Studies of tolerance to organ antigens. The immune response to A/Jax strain mouse organs in normal and A/Jax strain mouse liver tolerant rabbits

1971 ◽  
Vol 17 (4) ◽  
pp. 467-476
Author(s):  
M. G. Baines ◽  
D. Eidinger
Keyword(s):  
Mouse Liver ◽  
Fluorescent Antibody ◽  
Antigenic Determinants ◽  
Specific Antibodies ◽  
Strain Mouse ◽  
Cell Structures ◽  
Organ Specific ◽  
Specific Antigens ◽  
Antibody Tests ◽  
Guinea Pig Liver

Neonatal rabbits were injected with large amounts of pooled normal A/Jax strain mouse liver and serum to induce tolerance to the organ and species antigens present in this mixture. At 3 months of age, the "tolerant" and uninjected control rabbits were all challenged with either A/Jax strain heart and (or) liver or kidney and their responses were compared by precipitation, hemagglutination (HA), and indirect fluorescent antibody tests (IFab). The normal sera showed one to four precipitation arcs on two-dimensional immunodiffusion analysis against their respective organ antigens and those demonstrable on immunoelectrophoresis migrated predominantly in the β and γ globulin regions. Organ-specific antibodies reacting with the organ antigens of the immunizing species and heterologous species were easily quantified by HA titrations and localized by IFab tests. It was noted that antigens common to the tissues of the immunizing species were found principally in the supportive cell structures whereas organ-specific antigens were found in the cytoplasmic region of the immunizing tissue and in similar tissues of different species.Antisera derived from the "tolerant" rabbits immunized with heart and (or) liver showed virtually normal heart specific responses while the response to liver was significantly suppressed. The complete absence of the cross reaction to guinea pig liver in the "tolerant" sera was taken as evidence of the tolerance mediated suppression of the reactivity to some of the mouse liver antigenic determinants.

Differentiation of allantoic endoderm implanted into the presumptive digestive area in avian embryos. A study with organ-specific antigens

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 137-153
Author(s):  
Sadao Yasugi
Keyword(s):  
Digestive Tract ◽  
Intestinal Epithelium ◽  
Digestive Organ ◽  
Chick Embryos ◽  
Avian Embryos ◽  
Digestive Organs ◽  
Organ Specific ◽  
Specific Antigens

Quail allantoic endoderm was implanted into the presumptive digestive-tract area of chick embryos, and the differentiation of the endoderm was examined morphologically and immunocytochemically with antisera against pepsinogens and sucrase. The allantoic endoderm was incorporated into the host digestive organs. It often became continuous with the host endoderm and formed a chimaeric digestive-tract epithelium. It differentiated morphologically into the epithelium of the digestive organ into which it was incorporated, showing the morphological inductive ability in situ of the digestive-tract mesenchyme against the allantoic endoderm. However, the allantoic endoderm did not produce pepsinogens even when it was incorporated into the host proventricular mesenchyme and formed well-developed proventricular glands. This result indicates that the heterotypic morphogenesis of the allantoic endoderm is not necessarily accompanied by the heterotypic cytodifferentiation. In contrast, the anti-sucrase antiserum-reactive cells often differentiated in the allantoic endoderm incorporated into not only the intestine but also other organs. This confirmed our previous observation that the allantoic endoderm has a tendency to differentiate into the intestinal epithelium in the heterologous environment.

Organ-specific gene masking in mammalian chromosomes

1970 ◽  
Vol 176 (1044) ◽  
pp. 277-285 ◽  
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Dna Hybridization ◽  
Mouse Liver ◽  
Early Event ◽  
Specific Gene ◽  
Histone Proteins ◽  
Organ Specific ◽  
Liver And Kidney

Chromatin (chromosomal nucleoprotein) from mammalian colls is used as a template for the synthesis of RNA which is characterized and compared with other RNA by RNA-DNA hybridization. It is found to be transcribed from a restricted set of sequences and cannot be distinguished from natural RNA from the same organ as the chromatin. In contrast, it is different from RNA from other organs. Hence, DNA is masked in an organ-specific way in vivo and the masking is preserved on isolation. When cell division is induced in mouse liver and kidney a very early event is a change in masking in chromatin. This precedes changes in RNA populations; both precede DNA synthesis. Chromatin can be accurately reconstructed from DNA, histones and non-histone proteins. Experiments using this system indicate that histones non-specifically mask DNA; non-histone proteins are essential to reverse masking in a specific way.

Species-and organ-specific antigens in tissue cultures of considerable age

1963 ◽  
Vol 54 (3) ◽  
pp. 1010-1013
Author(s):  
A. T. Kravchenko ◽  
N. A. Kolesnikova ◽  
G. T. Patrikeev
Keyword(s):  
Tissue Cultures ◽  
Organ Specific ◽  
Specific Antigens

Comparison of Glycoprotein Expression Between Ovarian and Colon Adenocarcinomas

1999 ◽  
Vol 123 (10) ◽  
pp. 909-916
Author(s):  
Hinke A. B. Multhaupt ◽  
Carmen P. Arenas-Elliott ◽  
Michael J. Warhol
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Malignant Transformation ◽  
Qualitative And Quantitative ◽  
Metastatic Lesions ◽  
Organ Specific ◽  
Specific Antigens ◽  
Keratin Proteins ◽  
Colonic Adenocarcinomas ◽  
Ovarian Adenocarcinomas

Abstract Objective.—Tumor-associated antigens may be expressed as surface glycoproteins. These molecules undergo qualitative and quantitative modifications during cell differentiation and malignant transformation. During malignant transformation, incomplete glycosylation is common, and certain glycosylation pathways are preferred. These antigens might help distinguish between ovarian and colonic adenocarcinomas in the primary and metastatic lesions. Different cytokeratins have been proposed as relatively organ-specific antigens. Design.—We used monoclonal antibodies against T1, Tn, sialosyl-Tn, B72.3, CA125, carcinoembryonic antigen, and cytokeratins 7 and 20 to detect tumor-associated glycoproteins and keratin proteins in ovarian and colonic carcinomas. Results.—CA125, carcinoembryonic antigen, and cytokeratins 7 and 20 can distinguish between colonic and serous or endometrioid adenocarcinomas of the ovary in both primary and metastatic lesions. Mucinous ovarian adenocarcinomas differed in that they express carcinoembryonic antigen and cytokeratins 7 and 20 and weakly express CA125. The other glycoprotein antigens were equally expressed by ovarian and colonic adenocarcinomas and therefore were of no use in distinguishing between these 2 entities. Conclusion.—A panel of monoclonal antibodies against cytokeratins 7 and 20 antigens, CA125, and carcinoembryonic antigen is useful in differentiating serous and endometrioid adenocarcinomas of the ovary from colonic adenocarcinomas. Mucinous ovarian adenocarcinomas cannot be distinguished from colonic adenocarcinomas using immunohistochemistry.

Localization of organ-specific antigens in the nervous system of the rat

1977 ◽  
Vol 39 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Halina Weinrauder ◽  
Boles?aw Lach
Keyword(s):  
Nervous System ◽  
Organ Specific ◽  
Specific Antigens
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