Species-and organ-specific antigens in tissue cultures of considerable age

1963 ◽  
Vol 54 (3) ◽  
pp. 1010-1013
Author(s):  
A. T. Kravchenko ◽  
N. A. Kolesnikova ◽  
G. T. Patrikeev
Keyword(s):  
Tissue Cultures ◽  
Organ Specific ◽  
Specific Antigens

Differentiation of allantoic endoderm implanted into the presumptive digestive area in avian embryos. A study with organ-specific antigens

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 137-153
Author(s):  
Sadao Yasugi
Keyword(s):  
Digestive Tract ◽  
Intestinal Epithelium ◽  
Digestive Organ ◽  
Chick Embryos ◽  
Avian Embryos ◽  
Digestive Organs ◽  
Organ Specific ◽  
Specific Antigens

Quail allantoic endoderm was implanted into the presumptive digestive-tract area of chick embryos, and the differentiation of the endoderm was examined morphologically and immunocytochemically with antisera against pepsinogens and sucrase. The allantoic endoderm was incorporated into the host digestive organs. It often became continuous with the host endoderm and formed a chimaeric digestive-tract epithelium. It differentiated morphologically into the epithelium of the digestive organ into which it was incorporated, showing the morphological inductive ability in situ of the digestive-tract mesenchyme against the allantoic endoderm. However, the allantoic endoderm did not produce pepsinogens even when it was incorporated into the host proventricular mesenchyme and formed well-developed proventricular glands. This result indicates that the heterotypic morphogenesis of the allantoic endoderm is not necessarily accompanied by the heterotypic cytodifferentiation. In contrast, the anti-sucrase antiserum-reactive cells often differentiated in the allantoic endoderm incorporated into not only the intestine but also other organs. This confirmed our previous observation that the allantoic endoderm has a tendency to differentiate into the intestinal epithelium in the heterologous environment.

Comparison of Glycoprotein Expression Between Ovarian and Colon Adenocarcinomas

1999 ◽  
Vol 123 (10) ◽  
pp. 909-916
Author(s):  
Hinke A. B. Multhaupt ◽  
Carmen P. Arenas-Elliott ◽  
Michael J. Warhol
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Malignant Transformation ◽  
Qualitative And Quantitative ◽  
Metastatic Lesions ◽  
Organ Specific ◽  
Specific Antigens ◽  
Keratin Proteins ◽  
Colonic Adenocarcinomas ◽  
Ovarian Adenocarcinomas

Abstract Objective.—Tumor-associated antigens may be expressed as surface glycoproteins. These molecules undergo qualitative and quantitative modifications during cell differentiation and malignant transformation. During malignant transformation, incomplete glycosylation is common, and certain glycosylation pathways are preferred. These antigens might help distinguish between ovarian and colonic adenocarcinomas in the primary and metastatic lesions. Different cytokeratins have been proposed as relatively organ-specific antigens. Design.—We used monoclonal antibodies against T1, Tn, sialosyl-Tn, B72.3, CA125, carcinoembryonic antigen, and cytokeratins 7 and 20 to detect tumor-associated glycoproteins and keratin proteins in ovarian and colonic carcinomas. Results.—CA125, carcinoembryonic antigen, and cytokeratins 7 and 20 can distinguish between colonic and serous or endometrioid adenocarcinomas of the ovary in both primary and metastatic lesions. Mucinous ovarian adenocarcinomas differed in that they express carcinoembryonic antigen and cytokeratins 7 and 20 and weakly express CA125. The other glycoprotein antigens were equally expressed by ovarian and colonic adenocarcinomas and therefore were of no use in distinguishing between these 2 entities. Conclusion.—A panel of monoclonal antibodies against cytokeratins 7 and 20 antigens, CA125, and carcinoembryonic antigen is useful in differentiating serous and endometrioid adenocarcinomas of the ovary from colonic adenocarcinomas. Mucinous ovarian adenocarcinomas cannot be distinguished from colonic adenocarcinomas using immunohistochemistry.

Localization of organ-specific antigens in the nervous system of the rat

1977 ◽  
Vol 39 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Halina Weinrauder ◽  
Boles?aw Lach
Keyword(s):  
Nervous System ◽  
Organ Specific ◽  
Specific Antigens

scholarly journals Self-reactive B Cells Are Not Eliminated or Inactivated by Autoantigen Expressed on Thyroid Epithelial Cells

1997 ◽  
Vol 186 (12) ◽  
pp. 2005-2012 ◽  
Author(s):  
Srinivas Akkaraju ◽  
Karen Canaan ◽  
Christopher C. Goodnow
Keyword(s):  
B Cells ◽  
Epithelial Cells ◽  
Thyroid Stimulating Hormone ◽  
Specific Cell ◽  
Cell Repertoire ◽  
Thyroid Cells ◽  
Organ Specific ◽  
Membrane Antigens ◽  
Specific Antigens ◽  
Humoral Tolerance

Graves' Disease results from the production of autoantibodies against receptors for thyroid stimulating hormone (TSH) on thyroid epithelial cells, and represents the prototype for numerous autoimmune diseases caused by autoantibodies that bind to organ-specific cell membrane antigens. To study how humoral tolerance is normally maintained to organ-specific membrane antigens, transgenic mice were generated selectively expressing membrane-bound hen egg lysozyme (mHEL) on the thyroid epithelium. In contrast to the deletion of autoreactive B cells triggered by systemic mHEL (Hartley, S.B., J. Crosbie, R. Brink, A.B. Kantor, A. Basten, and C.C. Goodnow. 1991. Nature. 353:765–769), selective expression of mHEL autoantigen on thyroid cells did not trigger elimination or inactivation of circulating HEL-reactive B cells. These results provide evidence that tolerance is not actively acquired to organ-specific antigens in the preimmune B cell repertoire, underscoring the importance of maintaining tolerance to such antigens by other mechanisms. The role of an intact endothelial barrier in sequestering organ-specific antigens from circulating preimmune B cells is discussed.

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