Automated HPLC-amino acid determination of protein hydrolysates by precolumn derivatization with OPA and FMOC and comparison with classical ion exchange chromatography

1991 ◽  
Vol 31 (9-10) ◽  
pp. 441-447 ◽  
Author(s):  
U. Bütikofer ◽  
D. Fuchs ◽  
J. O. Bosset ◽  
W. Gmür
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Protein Hydrolysates ◽  
Precolumn Derivatization ◽  
Amino Acid Determination ◽  
Automated Hplc ◽  
Acid Determination

Isolation and structure determination of the peptides from the chymotryptic hydrolysate of the alkaline protease from Aspergillus flavus

1980 ◽  
Vol 45 (7) ◽  
pp. 1996-2028 ◽  
Author(s):  
Otakar Mikeš ◽  
Jaroslava Turková ◽  
Geoffrey Allen ◽  
Nguyen bao Toan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Aspergillus Flavus ◽  
Alkaline Protease ◽  
Paper Electrophoresis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Trichloracetic Acid

From the chymotryptic hydrolysate of 1.08 g of the precipitate obtained on treatment of alkaline protease from Aspergillus flavus with trichloracetic acid 134 peptides were isolated by means of ion exchange chromatography, paper electrophoresis and paper chromatography. Among these 38 peptides containing 311 amino acids were isolated in amounts exceeding 0.50μmol. The peptides were characterized by amino acid analysis, electric charge and also mostly by the terminal groups determination. In the case of peptides isolated in larger amounts the complete or at least the partial sequence of amino acids has been determined. In all peptides the total isolated amount in μmol was determined. The peptides containing basic amino acids were subfractionated with trypsin.

scholarly journals Determination of free amino acids in plants by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

2015 ◽  
Vol 7 (18) ◽  
pp. 7574-7581 ◽  
Author(s):  
Magdalena M. Dziągwa-Becker ◽  
Jose M. Marin Ramos ◽  
Jakub K. Topolski ◽  
Wiesław A. Oleszek
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Tandem Mass Spectrometry ◽  
Free Amino ◽  
Tandem Mass ◽  
Amino Acid Determination ◽  
Acid Determination

Free amino acid determination in plants by LC-MS/MS.

Determination of homocysteine in plasma by ion-exchange chromatography

1989 ◽  
Vol 49 (5) ◽  
pp. 445-449 ◽  
Author(s):  
A. Andersson ◽  
L. Brattström ◽  
A. Isaksson ◽  
B. Israelsson ◽  
B. Hultberg
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography
Sign in / Sign up

Export Citation Format

Share Document