Effect of riboflavin deficiency on free amino acid nitrogen concentrations of liver, muscle and plasma

1968 ◽  
Vol 24 (8) ◽  
pp. 786-787 ◽  
Author(s):  
A. K. Chatterjee ◽  
B. B. Ghosh
1986 ◽  
Vol 16 (5) ◽  
pp. 897-902 ◽  
Author(s):  
H. A. Margolis ◽  
R. H. Waring

Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings at a western Oregon nursery were fertilized in October 1983 with ammonium nitrate and harvested for biochemical analyses on four dates over autumn and winter 1983–1984. Free amino acid and total nitrogen concentrations in the needles of fertilized seedlings showed a pronounced increase 1 month after fertilization. Free amino acid concentrations of fertilized seedlings decreased in needles during winter but remained stable in stems and fine roots. Just before budbreak in mid-March, free amino acid concentrations increased significantly in stems and fine roots. Total nitrogen concentrations increased 1 month after fertilization, remained stable throughout winter, and tended to decrease or remain stable just before budbreak. Starch and total nonstructural carbohydrate concentrations of needles and stems of fertilized seedlings were lower just before budbreak and sugar concentrations of fine roots of fertilized seedlings were lower when data from all harvests were combined. The reduction in carbohydrate reserves following fertilization probably reflects increased respiration associated with the synthesis and maintenance of higher levels of enzymes.


1980 ◽  
Vol 4 (6) ◽  
pp. 548-553 ◽  
Author(s):  
David B. A. Silk ◽  
Peter D. Fairclough ◽  
Michael L. Clark ◽  
John E. Hegarty ◽  
Jill M. Addison ◽  
...  

1984 ◽  
Vol 247 (1) ◽  
pp. E75-E83 ◽  
Author(s):  
F. Muhlbacher ◽  
C. R. Kapadia ◽  
M. F. Colpoys ◽  
R. J. Smith ◽  
D. W. Wilmore

The effects of dexamethasone on nitrogen and amino acid metabolism in the dog were studied in order to gain insight into the role of glucocorticoids in accelerated proteolysis and altered metabolism of glutamine in catabolic illnesses. After dexamethasone administration at a dose of 0.44 mg X day-1 X kg-1, nitrogen balance shifted from slightly positive (+0.126 g N X day-1 X kg-1) to markedly negative (-0.278 g N X day-1 X kg-1). This was associated with a 23% fall in total free amino acid nitrogen in skeletal muscle, with 80% of the decline accounted for by a decrease in glutamine. Plasma glutamine concentration decreased by 26%, although total plasma free amino acid nitrogen was unchanged because of a 49% increase in alanine. The alterations in intracellular and circulating levels of glutamine were not accompanied by measurable changes in glutamine synthetase or glutaminase activities in skeletal muscle. Hindquarter amino acid flux measurements demonstrated that the decline in intracellular glutamine concentration was associated with a marked increase in glutamine efflux from skeletal muscle. This occurred in spite of minimal changes in the intracellular/extracellular glutamine gradient. It is concluded that accelerated muscle glutamine release caused by glucocorticoids is a major contributor to the decreased glutamine levels in muscle that occur during critical illnesses.


Author(s):  
Pritha Das ◽  
Sudipta Pal

Objective: The study was conducted to evaluate the dose-dependent effects of sub-acute lead exposure on certain aspects of carbohydrate metabolism.Methods: Swiss albino male mice (weighing 30-35 g) were selected for the present study and divided into five groups; one control group and others lead-treated groups i.e. Group A (5 mg/kg body weight), Group B (10 mg/kg body weight), Group C (15 mg/kg body weight) and Group D (20 mg/kg body weight). Parameters like blood and liver glucose, glycogen and pyruvic acid contents were determined in liver tissue. The enzyme activities like pyruvate dehydrogenase, malate dehydrogenase and glucose 6-phosphatase were recorded in that tissue. Additionally, free amino acid nitrogen content and transaminase enzyme activities were also evaluated in liver tissue of mice.Results: The study reveals that lead caused a significant diminution of blood and hepatic glucose levels and fall in liver glycogen content in a dose-dependent manner, the highest effect was observed in animals treated with lead at a dose of 20 mg/kg body weight. Glucose 6-phosphatase activity was decreased significantly in all the treated groups. There was a dose-dependent increase in pyruvic acid content whereas pyruvate dehydrogenase, malate dehydrogenase and transaminase enzyme activities were significantly depressed in a dose-dependent fashion in all the treated animals. Additionally, lead treatment significantly (p<0.001) enhanced free amino acid nitrogen in the liver to provide a substrate for gluconeogenesis.Conclusion: It is suggested that an adaptive mechanism is initiated by stimulating and retarding glycogenolytic and glycolytic activity and also by rising in the content of free amino acid nitrogen to recover from the lead stressed toxic manifestation


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
EJ Cho ◽  
XL Piao ◽  
MH Jang ◽  
SY Park ◽  
SW Kwon ◽  
...  

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
BM Silva ◽  
AP Oliveira ◽  
DM Pereira ◽  
C Sousa ◽  
RM Seabra ◽  
...  

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