Potato composition: III. Tissue selection and its effects on total nitrogen, free amino acid nitrogen and enzyme activity (polyphenolase, monophenolase, peroxidase and catalase)

1978 ◽  
Vol 55 (6) ◽  
pp. 319-331 ◽  
Author(s):  
M. L. Weaver ◽  
H. Timm ◽  
M. Nonaka ◽  
R. N. Sayre ◽  
K. C. Ng ◽  
...  
Author(s):  
C. B. Cowey ◽  
E. D. S. Corner

Amino acids, both free and combined as protein, and some other nitrogenous constituents of Calanus finmarchicus have been examined. Seventy-six per cent of the total nitrogen in C. finmarchicus was present in protein amino acids, 14% in the free amino acid fraction, 6% in trimethylamine oxide, and 1·5% in betaine. These findings are discussed in relation to previous work on nitrogenous constituents of Calanus.The free amino acid fraction of Calanus is compared with that of higher Crustacea and it is suggested that this fraction may be important in the adaptation of the animal to dilute sea water.Amino acid nitrogen made up 40% of the total nitrogen of particulate material from Clyde sea water. This finding is compared with other recent analyses of the phytoplanktonic nitrogen and total particulate nitrogen of inshore waters.


1979 ◽  
Vol 51 (1) ◽  
pp. 506-513
Author(s):  
Terttu Ettala ◽  
Matti Kreula

The nitrogen compounds of the faeces of dairy cows on purified, protein-free feed with urea and small amounts of ammonium salts as the sole source of nitrogen (0-feed). on low-protein feed in which part of the protein was replaced with urea (ULP-feed) and on normal protein-containing feed (NorP-feed) were studied. The total nitrogen contents of the dry matter of the faeces were 2.5 ± 0.7 % on 0-feed, 2.4 ± 0.4 % on ULP-feed and 2.3 ± 0.6 % on NorP-feed. The proportion of urea- and ammonium nitrogen of the total nitrogen was 3.2 ± 2.4, 4.5 ± 2.4 and 3.7 ± 1.8 % respectively, and the proportion of nitrogen soluble in 70 % ethanol 10.9 ± 5.7, 9.9 ± 2.4 and 10.4 ± 3.8 % of the total nitrogen; the proportion of nitrogen in the free amino acid fraction was 4.1 ±2.9 on 0-feed, 1.6 ± 1.4 (P < 0.05) on ULP-feed and 3.2 ± 2.9 % on NorP-feed. The nitrogen insoluble in 70 % ethanol as a proportion of the total nitrogen was 82.3 ± 7.2 on 0-feed, 85.4 ± 5.0 on ULP-feed and 82.3 ± 7.2 % on NorP-feed. Of the total nitrogen of this insoluble fraction, the following proportions were released as amino acids by acid hydrolysis: 62.15 ± 8.5 % on 0-feed, 61.6 ± 10.7 % on ULP-feed and 59.0 ± 13.9% on NorP-feed. (α, Ɛ-diaminopimelic acid was 1.7 ± 0.6, 0.7 ± 0.2 (P < 0.05) and 1.2 ± 0,4 mol % respectively of the total amino acids of the hydrolyzate. The soluble nitrogen fraction was separated into free amino acid and »peptide» fractions, and the amino acid composition of both was determined, the latter after acid hydrolysis


1986 ◽  
Vol 16 (5) ◽  
pp. 897-902 ◽  
Author(s):  
H. A. Margolis ◽  
R. H. Waring

Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings at a western Oregon nursery were fertilized in October 1983 with ammonium nitrate and harvested for biochemical analyses on four dates over autumn and winter 1983–1984. Free amino acid and total nitrogen concentrations in the needles of fertilized seedlings showed a pronounced increase 1 month after fertilization. Free amino acid concentrations of fertilized seedlings decreased in needles during winter but remained stable in stems and fine roots. Just before budbreak in mid-March, free amino acid concentrations increased significantly in stems and fine roots. Total nitrogen concentrations increased 1 month after fertilization, remained stable throughout winter, and tended to decrease or remain stable just before budbreak. Starch and total nonstructural carbohydrate concentrations of needles and stems of fertilized seedlings were lower just before budbreak and sugar concentrations of fine roots of fertilized seedlings were lower when data from all harvests were combined. The reduction in carbohydrate reserves following fertilization probably reflects increased respiration associated with the synthesis and maintenance of higher levels of enzymes.


1980 ◽  
Vol 4 (6) ◽  
pp. 548-553 ◽  
Author(s):  
David B. A. Silk ◽  
Peter D. Fairclough ◽  
Michael L. Clark ◽  
John E. Hegarty ◽  
Jill M. Addison ◽  
...  

1991 ◽  
Vol 41 (3) ◽  
pp. 301-306
Author(s):  
Hiroshi TERAI ◽  
Daisuke HINODE ◽  
Tomie MAN-YOSHI ◽  
Atsushi NAGATA ◽  
Makoto SATO ◽  
...  

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