Comparison of polymerase chain reaction with standard methods in the diagnosis ofMycobacterium tuberculosis infection

1994 ◽  
Vol 13 (12) ◽  
pp. 1079-1083 ◽  
Author(s):  
J. Pietrzak ◽  
R. Frei ◽  
H. P. Senn ◽  
C. Moroni
Keyword(s):  
Polymerase Chain Reaction ◽  
Tuberculosis Infection ◽  
Chain Reaction ◽  
Standard Methods ◽  
Ofmycobacterium Tuberculosis ◽  
Polymerase Chain

Detection of Mycobacterium tuberculosis in Clinical Specimens From Children Using a Polymerase Chain Reaction

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Kim Connelly Smith ◽  
Jeffrey R. Starke ◽  
Kathleen Eisenach ◽  
Lydia T. Ong ◽  
Melissa Denby
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Clinical Diagnosis ◽  
Tuberculosis Infection ◽  
Chain Reaction ◽  
Clinical Specimens ◽  
Mycobacterial Culture ◽  
Polymerase Chain ◽  
Tuberculosis In Children ◽  
Culture Negative

Objective. We evaluated the usefulness of the polymerase chain reaction (PCR) using the insertion sequence IS6110 as the target for DNA to detect Mycobacterium tuberculosis in clinical specimens from children. Study Design. This was a prospective, controlled, blinded study comparing PCR on clinical specimens, mycobacterial culture, and clinical diagnosis. Patients. Sixty-five hospitalized children were evaluated, 35 with tuberculosis disease and 30 controls. Cases were defined by culture and/or specific clinical criteria. Controls included patients with tuberculosis infection but no detectable disease as well as patients free of tuberculosis infection and disease. Results. Polymerase chain reaction had a sensitivity of 40% and a specificity of 80% compared with clinical diagnosis. Mycobacterial culture had a sensitivity of 37%. The combination of culture and PCR identified 19 of 35 children (54%) with clinically diagnosed tuberculosis. There were six children with false-positive PCR results: One had tuberculosis infection without disease, two had Mycobacterium avium lymphadenitis, and three had diagnoses unrelated to tuberculosis. Conclusions. The sensitivity of PCR is comparable to that of culture for detecting M tuberculosis in children, and may strengthen and hasten the clinical diagnosis in culture-negative patients. However, because of the limitations in specificity, the results of PCR alone are insufficient to diagnose tuberculosis in children. Although ongoing refinements in PCR techniques should improve the specificity of this test, epidemiologic and clinical information continue to be the most important consideration in the diagnosis of tuberculosis in culture-negative children.

scholarly journals Direct diagnosis ofMycobacterium tuberculosis in blood samples of HIV infected patients by polymerase chain reaction

2000 ◽  
Vol 15 (2) ◽  
pp. 76-82 ◽  
Author(s):  
Senthilkumar Kamatchiammal ◽  
Dhashinamoorthy Saravanakumar ◽  
Nagalingeswaran Kumarasamy ◽  
Sunithi Solomon ◽  
Manjula Sritharan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Blood Samples ◽  
Ofmycobacterium Tuberculosis ◽  
Polymerase Chain

Polymorphism of the gene pol of the bovine leukemia virus

2014 ◽  
Vol 1 (3) ◽  
pp. 22-25
Author(s):  
A. Gerilovych ◽  
B. Stegniy ◽  
O. Limanska ◽  
S. Gorbatenko
Keyword(s):  
Polymerase Chain Reaction ◽  
Sequence Analysis ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
European Part ◽  
Chain Reaction ◽  
Standard Methods ◽  
The Russian Federation ◽  
Polymerase Chain ◽  
Reaction Sequencing

As a representative of RNA-containing viruses, the causative agent of bovine leukemia (BLV) is characterized by the signifi cant level of genetic variability which results in considerable information content of phylogenic studies of its populations. Aim. This study is aimed at exploring the sequence of 440 bp long fragment of the pol gene of BLV in three farms in the Eastern Ukraine. Methods. The polymerase chain reaction, sequencing and standard methods of isolating nucleic acids have been used. Results. The sequencing and the sequence analysis of the pol gene demonstrated the presence of three variants of sequences, corresponding to the isolates, identifi ed in Kharkiv and Sumy regions and the sample of the proviral DNA from the FLK-BLV culture. Con- clusions. The genetic homology of the sequences of the characterized isolates to the populations of viruses, circulating in the territory of Europe and the European part of the Russian Federation, has been established.

Sign in / Sign up

Export Citation Format

Share Document