Endogenous splenic colonies and megakaryopoiesis in methylcellulose treated irradiated mice

1985 ◽  
Vol 41 (1) ◽  
pp. 97-99 ◽  
Author(s):  
P. A. Bernabei ◽  
S. Di Lollo ◽  
R. Saccardi ◽  
L. Arganini ◽  
A. Pecoraro ◽  
...  
Keyword(s):  
Splenic Colonies ◽  
Endogenous Splenic Colonies

Failure of mice infected with Rauscher leukemia virus (RLV-A) to develop splenic colonies

1977 ◽  
Vol 9 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Alan Bergson ◽  
Joseph Lobue ◽  
Torgny N. Fredrickson ◽  
Albert S. Gordon
Keyword(s):  
Leukemia Virus ◽  
Rauscher Leukemia ◽  
Splenic Colonies ◽  
Rauscher Leukemia Virus

The Role of the Lymphocyte in Haemopoiesis

1979 ◽  
Vol 24 (4) ◽  
pp. 267-272 ◽  
Author(s):  
Ronald D. Barr
Keyword(s):  
Surface Membrane ◽  
Culture Conditions ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Colony Forming Units ◽  
The Family ◽  
Functional Control ◽  
Splenic Colonies ◽  
Exhaustive Study ◽  
Self Replication

According to current dogma, circulating blood cells are all derived from the same progenitor, which therefore must be both pluripotent and capable of prolific self-replication. In the irradiated mouse, such haemopoietic stem cells (HSC) give rise to splenic colonies, and thus are designated as CFU-S (colony forming units-spleen). Definitive identification of a similar entity in man so far has proved elusive. However, primitive unipotent cells, committed to development along a single pathway, can be detected in human blood-forming tissues under appropriate culture conditions. Operationally defined as CFU-E (erythrocyte), CFU-GM (granulocyte/macrophage) etc., the ontogenetic relationships of these cells to each other and to the HSC have been the objects of exhaustive study. A population of lymphocytes, classed as ‘null’ cells, do not exhibit the surface membrane markers which characterize commitment to differentiation in the thymusdependent (T-cell) or bursa- equivalent (B cell) lineages. Accumulating evidence points to the null lymphocyte as a potential precursor of haemopoietic tissue. In bone marrow, the activity of terminal deoxynucleotidyl transferase (TdT) is concentrated in null cells, but the relevance of this unique enzyme to lymphocytic differentiation remains uncertain. Nevertheless, it appears that the functional heterogeneity, expressed within the family of lymphocytes, extends to haemopoiesis. The roles of various lymphocyte populations, in the generation and functional control of blood-forming tissue, are examined in this review.

Effect of regeneration of hematopoietic organs on the number and type of splenic colonies

1978 ◽  
Vol 85 (2) ◽  
pp. 223-225
Author(s):  
G. K. Sukhova ◽  
T. R. Podrabinek ◽  
G. V. Kharlova
Keyword(s):  
Hematopoietic Organs ◽  
Splenic Colonies

scholarly journals T- and B-lymphocyte differentiation potentials of spleen colony-forming cells

Blood ◽  
1993 ◽  
Vol 81 (4) ◽  
pp. 950-955 ◽  
Author(s):  
F Lepault ◽  
S Ezine ◽  
MC Gagnerault
Keyword(s):  
B Lymphocytes ◽  
B Lymphocyte ◽  
Marrow Cell ◽  
Cell Transfer ◽  
Lymphocyte Differentiation ◽  
Colony Forming Units ◽  
Donor Type ◽  
Splenic Colonies ◽  
Intrathymic Injection ◽  
Open Question

Abstract Cells that generate splenic colonies within 8 days (day-8 colony- forming units-spleen [CFU-s]) are generally thought to differentiate only into erythroid/myeloid cells. The T and B lymphocyte differentiation potentials of day-8 CFU-s were evaluated and compared with those of day-12 and 5-fluorouracil (5-FU) CFU-s. This was achieved by analyzing, after intravenous and intrathymic injection, the lymphocyte progeny of cells contained within individual splenic colonies collected at day 8 and day 12 post-bone marrow cell transfer into irradiated congenic recipients. A large majority of day-8 spleen colonies generated T cells when transferred intrathymically. After intravenous (IV) injection of day-8 colonies, donor-type thymocytes emerged in 33% of the animals reconstituted with only 1 day-8 colony, but in 83% of those inoculated with a pool of 5 colonies. All post-5-FU and 75% of day-12 colonies gave rise to thymocytes after IV injection. B cells were generated by a high proportion of day-8 colonies, and by all day-12 and post 5-FU colonies. These results demonstrate that progenitors of T and B lymphocytes are generated within spleen colonies produced by at least some day-8 CFU-s and virtually all day-12 CFU-s. Whether these progenitors are CFU-s themselves or committed precursors remains an open question.

Precursors of transient splenic colonies in the mouse embryonic liver

1984 ◽  
Vol 97 (6) ◽  
pp. 806-808
Author(s):  
O. I. Gan ◽  
N. I. Drize ◽  
T. V. Todriya ◽  
I. L. Chertkov
Keyword(s):  
Embryonic Liver ◽  
Splenic Colonies

scholarly journals Stem cell quality following irradiation: Comparison of 125IUdR uptake in the spleen and number and size of splenic colonies in bone marrow transfused, fatally irradiated mice.

1984 ◽  
Vol 25 (4) ◽  
pp. 261-273 ◽  
Author(s):  
T. INOUE ◽  
E. P. CRONKITE ◽  
G. E. HVBNER ◽  
K.-H. V. WANGENHEIM ◽  
L. E. FEINENDEGEN
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Splenic Colonies

scholarly journals The Effect of Chloramphenicol and Thiamphenicol on the Ultrastructure of the Erythroblasts in Mouse Splenic Colonies

1980 ◽  
Vol 130 (4) ◽  
pp. 335-340 ◽  
Author(s):  
AKIRA B. MIURA ◽  
KOSAKU YOSHIDA ◽  
AKIHIKO YAMAGUCHI ◽  
MITSUYUKI FUKUDA
Keyword(s):  
Splenic Colonies

scholarly journals Annulate Lamellae in Erythroblasts in the Mice Splenic Colonies

1978 ◽  
Vol 124 (4) ◽  
pp. 387-390 ◽  
Author(s):  
AKIRA B. MIURA ◽  
KOSAKU YOSHIDA ◽  
AKIHIKO YAMAGUCHI ◽  
MITSUYUKI FUKUDA
Keyword(s):  
Annulate Lamellae ◽  
Splenic Colonies

Clones of antigen-binding cells in hematopoietic splenic colonies

1976 ◽  
Vol 81 (5) ◽  
pp. 720-723
Author(s):  
I. V. Khazanova ◽  
L. V. Van'ko ◽  
V. V. Malaitsev ◽  
N. S. Khamitova ◽  
G. M. Zhuravel' ◽  
...  
Keyword(s):  
Antigen Binding ◽  
Splenic Colonies
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